Advances in laboratory detection methods and technology application of SARS‐CoV‐2

Abstract

At present, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is raging worldwide, and the coronavirus disease 2019 outbreak caused by SARS-CoV-2 seriously threatens the life and health of all humankind. There is no specific medicine for novel coronavirus yet. So, laboratory diagnoses of novel coronavirus as soon as possible and isolation of the source of infection play a vital role in preventing and controlling the epidemic. Therefore, selecting appropriate detection techniques and methods is particularly important to improve the efficiency of disease diagnosis and treatment and to curb the outbreak of infectious diseases. In this paper, virus nucleic acid, protein, and serum immunology were reviewed to provide a reference for further developing virus detection technology to provide better prevention and treatment strategies and research ideas for clinicians and researchers.

Booster vaccination strategy: Necessity, immunization objectives, immunization strategy, and safety

“…47 Of course, if conditions permit, the detection and evaluation of antibody titers before vaccination will undoubtedly make this study more targeted. 48 The timing of the booster may also need to be shorter than 6 months.…”

“…The United Kingdom Joint Committee on Vaccination and Immunization suggested that people with immunodeficiency (including patients with advanced HIV, leukemia, organ transplantation, or immunosuppressive drugs) should be taken as the targets of a booster vaccination 47 . Of course, if conditions permit, the detection and evaluation of antibody titers before vaccination will undoubtedly make this study more targeted 48 . The timing of the booster may also need to be shorter than 6 months.…”

Performance and application evaluation of SARS‐CoV‐2 antigen assay

“…At present, the gold standard of COVID‐19 diagnosis is nucleic acid detection of SARS‐CoV‐2, which needs to be performed by specialized personnel in a polymerase chain reaction (PCR) laboratory with a second‐class biosafety level. 16 , 17 , 18 , 19 Therefore, it cannot be widely promoted due to the high requirements of staff skills and site requirements, especially in some grassroots medical institutions. In addition, it takes 4–6 h to complete the whole detection process of SARS‐CoV‐2 nucleic acid detection, and it takes a longer time when the number of specimens is large, leading to delays in the identification of COVID‐19 patients in some cases.…”

SARS-CoV-2 Spreads Globally Through the Object-to-Human Transmission of Cross-Border Logistics

“…COVID-19 is a public health emergency with the fastest transmission speed, the broadest range of infection, and the most challenging prevention and control in the past 100 years ( Ye et al, 2020a , 2021 ; Chen et al, 2021 ; Tian et al, 2021a , b ; Han and Ye, 2022 ; Zhang et al, 2022 ), and it can cause multiple organ damage or even death ( Tian and Ye, 2020 ; Ye et al, 2020a , b , c ; Han and Ye, 2021 ; Han et al, 2021 ; Khodavirdipour et al, 2021a , b ; Tian et al, 2021b ). Vaccines and drugs are being developed ( Khodavirdipour et al, 2020 , 2021c , 2022 ; Khodavirdipour, 2021 ).…”