Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Zhu, Xiaoping; Xu, Tianyi; Chen, Peng; Wu, Shihua

doi:10.3389/fchem.2021.782432

Cited by 67 publications

(57 citation statements)

References 217 publications

(245 reference statements)

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“…These modern multi-omics atlases build upon the extensive knowledge generated from large-scale descriptive studies that for instance mapped the 3D ultrastructure of a whole fly brain (61), or the early human development imaged by light-sheet microscopy (62), by adding a layer of functional data – a combination of structure and function. Still, despite rapid improvements on the technological side in terms of imaging speeds, spatial resolution, data processing and cost (16), there are still major challenges making large-scale correlative studies difficult. Besides harmonization of sample processing workflows for both 3D imaging techniques, the correlative analysis between modalities remains the most critical step.…”

Section: Resultsmentioning

confidence: 99%

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Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

Geier

Mansilla

Liutkevičiūtė

et al. 2022

Preprint

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Neuropeptides are important regulators of animal physiology and behavior. Hitherto large-scale localization of neuropeptides mainly relied on immunohistochemical methods requiring the availability of antibody panels, while another limiting factor has been the brain's opacity for subsequent light or fluorescence microscopy. To address these limitations, we integrated high-resolution mass spectrometry imaging (MSI) with microtomography for a multiplexed mapping of neuropeptides in two evolutionary distant ant species, Atta sexdens and Lasius niger. For analyzing the spatial distribution of chemically diverse peptide molecules across the brain in each species, the acquisition of serial mass spectrometry images was essential. As a result, we have comparatively mapped the 3D distributions of eight conserved neuropeptides throughout the brain micro-anatomy. We demonstrate that integrating the 3D MSI data into high-resolution anatomy models can be critical for studying organs with high plasticity such as brains of social insects. Several peptides, like the tachykinin-related peptides TK1 and TK4, were widely distributed in many brain areas of both ant species, whereas others, for instance myosuppressin was restricted to specific regions only. Also, we detected differences at the species level; many peptides were identified in the optic lobe of L. niger, but only one peptide (ITG-like) was found in this region in A. sexdens. Our approach provides the basis for investigating fundamental neurobiological processes by visualizing the unbiased 3D neurochemistry in its complex anatomic environment. Significance statement Until recently, the inability to detect entire molecules such as neuropeptides within their spatial biological context and simultaneously link their occurrence to anatomically and physiologically relevant areas has limited our understanding of complex neurochemical processes. This situation has now changed dramatically with the optimization of a new multiplexed imaging method based on mass spectrometry, which enables us to study previously invisible processes at the microscopic scale. With the marriage of mass spectrometry imaging and microtomography, we show that it has become possible to build high-resolution maps of neuropeptides in complex anatomical structures as small as ant brains. These maps, embedded in the 3D neuroanatomy, expand the understanding of the spatial organization of brain chemistry and provide a baseline for neurobiological and neurochemical studies.

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Section: Resultsmentioning

confidence: 99%

“…Mass-spectrometry imaging (MSI) in contrast is a multiplexed and label-free technique, which can map the spatial distribution and relative abundance of hundreds to thousands of molecules from the same tissue section (16). Over the last decade, matrix-assisted laser desorption/ionization (MALDI)-MSI has revolutionized spatial biology (17).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

Geier

Mansilla

Liutkevičiūtė

et al. 2022

Preprint

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“…Herein, we highlight two MSI approaches: Matrix-Assisted Laser Desorption/ionization and secondary ion mas spectrometry (SIMS). For technical details, we refer readers to recent reviews ( Watrous and Dorrestein, 2011 ; Dunham et al, 2017 ; Mayali, 2020 ; Zhu et al, 2021 ).…”

Section: Imaging and Measuring Microbial Activity And Interactions By...mentioning

confidence: 99%

One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology

2022

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Studying microbes from a single-cell perspective has become a major theme and interest within the field of aquatic microbiology. One emerging trend is the unfailing observation of heterogeneity in activity levels within microbial populations. Wherever researchers have looked, intra-population variability in biochemical composition, growth rates, and responses to varying environmental conditions has been evident and probably reflect coexisting genetically distinct strains of the same species. Such observations of heterogeneity require a shift away from bulk analytical approaches and development of new methods or adaptation of existing techniques, many of which were first pioneered in other, unrelated fields, e.g., material, physical, and biomedical sciences. Many co-opted approaches were initially optimized using model organisms. In a field with so few cultivable models, method development has been challenging but has also contributed tremendous insights, breakthroughs, and stimulated curiosity. In this perspective, we present a subset of methods that have been effectively applied to study aquatic microbes at the single-cell level. Opportunities and challenges for innovation are also discussed. We suggest future directions for aquatic microbiological research that will benefit from open access to sophisticated instruments and highly interdisciplinary collaborations.

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“…Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful label-free approach that allows imaging of many different molecular classes, including metabolites/pharmaceuticals, lipids, − peptides/proteins, , and N-glycans, throughout biological samples such as thin tissue sections, plants, and single cells. , In recent years, the spatial resolution of commercial MALDI-MSI systems has improved dramatically to 5–10 μm, with bespoke systems capable of reaching ∼1 μm spatial resolution. ,− Lipids are one of the most widely interrogated biomolecules by MALDI-MSI, along with other common MSI methods, such as secondary ion mass spectrometry (SIMS) − and desorption electrospray ionization (DESI). , This is due to both the relative ease of ionization of several common lipid classes as well as the central role of lipid metabolism in countless biological processes and diseases. ,, …”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Imaging of Lipids Using MALDI Coupled with Plasma-Based Post-Ionization on a Trapped Ion Mobility Mass Spectrometer

et al. 2022

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Here we report the development and optimization of a mass spectrometry imaging (MSI) platform that combines an atmospheric-pressure matrix-assisted laser desorption/ionization platform with plasma postionization (AP-MALDI-PPI) and trapped ion mobility spectrometry (TIMS). We discuss optimal parameters for operating the source, characterize the behavior of a variety of lipid classes in positive- and negative-ion modes, and explore the capabilities for lipid imaging using murine brain tissue. The instrument generates high signal-to-noise for numerous lipid species, with mass spectra sharing many similarities to those obtained using laser postionization (MALDI-2). The system is especially well suited for detecting lipids such as phosphatidylethanolamine (PE), as well as numerous sphingolipid classes and glycerolipids. For the first time, the coupling of plasma-based postionization with ion mobility is presented, and we show the value of ion mobility for the resolution and identification of species within rich spectra that contain numerous isobaric/isomeric signals that are not resolved in the m/z dimension alone, including isomeric PE and demethylated phosphatidylcholine lipids produced by in-source fragmentation. The reported instrument provides a powerful and user-friendly approach for MSI of lipids.

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Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Cited by 67 publications

References 217 publications

Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology

Mass Spectrometry Imaging of Lipids Using MALDI Coupled with Plasma-Based Post-Ionization on a Trapped Ion Mobility Mass Spectrometer

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