Advances in Microfluidics‐Based Technologies for Single Cell Culture

A review on microfluidic technology for antibacterial resistance study and antibiotic susceptibility testing (AST) is presented here. Antibiotic resistance has become a global health crisis in recent decades, severely threatening public health, patient care, economic growth, and even national security. It is extremely urgent that antibiotic resistance be well looked into and aggressively combated in order for us to survive this crisis. AST has been routinely utilized in determining bacterial susceptibility to antibiotics and identifying potential resistance. Yet conventional methods for AST are increasingly incompetent due to unsatisfactory test speed, high cost, and deficient reliability. Microfluidics has emerged as a powerful and very promising platform technology that has proven capable of addressing the limitation of conventional methods and advancing AST to a new level. Besides, potential technical challenges that are likely to hinder the development of microfluidic technology aimed at AST are observed and discussed. To conclude, it is noted that (1) the translation of microfluidic innovations from laboratories to be ready AST platforms remains a lengthy journey and (2) ensuring all relevant parties engaged in a collaborative and unified mode is foundational to the successful incubation of commercial microfluidic platforms for AST.

show abstract

“…for cell growth. A few commonly used microfluidic methods for single-cell isolation and culture are summarized very recently …”

Section: Significance Of Microfluidics To Antibacterial Resistance St...mentioning

confidence: 99%

Microfluidic Technology for Antibacterial Resistance Study and Antibiotic Susceptibility Testing: Review and Perspective

Ning

Zhao

et al. 2020

ACS Sens.

View full text Add to dashboard Cite

show abstract

“…Microfluidic technology has the ability to incorporate onto a single and fully automated chip multiple steps and control systems of benchtop laboratory protocols from sample mixing, separation, capture and detection, control and readout components through reliable temperature control sensors, valves, pumps, and among others. [ 214b ] Further, the possible assembly of an array of individual or connected controllable cell culture microchambers in a single platform enables an improved parallelization and reproducibility of assays in a high throughput manner for individual or multi cells/organs‐on‐a‐chip [ 217e,221,222 ] models, as well as a reduction in cost and waste produced because of the small amount required, in the nL‐range, pL‐range, and even fL‐range, of expensive samples and reagents. Furthermore, the low power consumption along with the cost effective manufacture of integrated systems using relatively low cost polymer materials provides opportunities for disposable and portable handheld devices that could potentially revolutionize the biomedical industry through unprecedented scientific research.…”

Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

“…Thus, this section will focused on some microfluidic bioreactors that study the effect of dynamic stimuli as indicative examples of so many others applications that can be found in a number of excellent papers, already mentioned through this review. [ 221,233a,242,274 ]…”

Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

Physically Active Bioreactors for Tissue Engineering Applications

et al. 2020

View full text Add to dashboard Cite

Tissue engineering (TE) is a dynamic and growing scientific field that merges the knowledge of different areas such as biology, physics, medicine, and engineering. [1] The TE discipline was first coined at a National Science Foundation sponsored meeting in 1987, thus originating a field that employs life sciences and engineering with the purpose of developing biologic or synthetic supports to restore, keep, or enhance tissue functions or damaged organs. [2] The classical TE approach has been mainly focusing on associating cells with a supporting matrix, also called biomaterials or scaffolds, that essentially acts as a passive template for tissue formation in vitro by allowing cells to adhere, migrate, differentiate, and produce tissue. Usually, the cells are seeded onto the scaffolds and, occasionally, growth factors are also added. The combination of cells, growth factors, and scaffold is often referred as the TE triad. [3] Nevertheless, novel approaches in TE that include the application of a biophysical stimuli to the scaffolds through the use of a bioreactor are emerging. [4] Tissue engineering (TE) is a strongly expanding research area. TE approaches require biocompatible scaffolds, cells, and different applied stimuli, which altogether mimic the natural tissue microenvironment. Also, the extracellular matrix serves as a structural base for cells and as a source of growth factors and biophysical cues. The 3D characteristics of the microenvironment is one of the most recognized key factors for obtaining specific cell responses in vivo, being the physical cues increasingly investigated. Supporting those advances is the progress of smart and multifunctional materials design, whose properties improve the cell behavior control through the possibility of providing specific chemical and physical stimuli to the cellular environment. In this sense, a varying set of bioreactors that properly stimulate those materials and cells in vitro, creating an appropriate biomimetic microenvironment, is developed to obtain active bioreactors. This review provides a comprehensive overview on the important microenvironments of different cells and tissues, the smart materials type used for providing such microenvironments and the specific bioreactor technologies that allow subjecting the cells/ tissues to the required biomimetic biochemical and biophysical cues. Further, it is shown that microfluidic bioreactors represent a growing and interesting field that hold great promise for achieving suitable TE strategies.

show abstract

“…Firstly, microfluidic chip is flexible in designing structures and functions to meet the demands of single cell analysis [ 14 ]. Secondly, typical microfluidic channels have dimension of tens to hundreds of microns that work from picoliter to nanoliter volumes of solution, enabling reduction of sample loss and high sensitivity, and making high-throughput single cell analysis possible [ 15 ]. In addition, the integration of multifunctional units and microfluidic chips can achieve automation, preventing measurement errors generated from human operations [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidics applications for high-throughput single cell sequencing

et al. 2021

View full text Add to dashboard Cite

The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individual cells. Single cell sequencing can provide data to characterize the inherent heterogeneity of individual cells, and reveal complex and rare cell populations. Different microfluidic technologies have emerged for single cell researches and become the frontiers and hot topics over the past decade. In this review article, we introduce the processes of single cell sequencing, and review the principles of microfluidics for single cell analysis. Also, we discuss the common high-throughput single cell sequencing technologies along with their advantages and disadvantages. Lastly, microfluidics applications in single cell sequencing technology for the diagnosis of cancers and immune system diseases are briefly illustrated.

show abstract

Advances in Microfluidics‐Based Technologies for Single Cell Culture

Cited by 21 publications

References 90 publications

Microfluidic Technology for Antibacterial Resistance Study and Antibiotic Susceptibility Testing: Review and Perspective

Microfluidic Technology for Antibacterial Resistance Study and Antibiotic Susceptibility Testing: Review and Perspective

Physically Active Bioreactors for Tissue Engineering Applications

Microfluidics applications for high-throughput single cell sequencing

Contact Info

Product

Resources

About