Advances in Molecular Diagnosis of Tuberculosis

Katoch, V. M.

doi:10.1016/s0377-1237(03)80001-1

Cited by 6 publications

(20 citation statements)

References 31 publications

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“…The genus mycobacterium consists of almost 100 different species, which all appear similar on AFB staining and culture [7,10,12]. Many of these can be isolated from humans, although many also can be found in the environment including in animals.…”

Section: Direct Detection Methodsmentioning

confidence: 99%

“…Each mycobacterium isolate must be evaluated individually regarding its potential to cause a disease; therefore identification of mycobactera is a lengthy and tedious effort. Since the introduction of nucleic acid amplification assays as diagnostic tool for mycobacteria identification, several probes/gene amplification systems for tuberculosis have been developed for rapid and specific identification of M. tuberculosis and other mycobacteria [12,13]. These techniques allow for the confirmation of identity of isolates, direct detection of gene sequences from the clinical specimens and also for molecular detection of drug resistance [12].…”

Section: Direct Detection Methodsmentioning

confidence: 99%

“…Since the introduction of nucleic acid amplification assays as diagnostic tool for mycobacteria identification, several probes/gene amplification systems for tuberculosis have been developed for rapid and specific identification of M. tuberculosis and other mycobacteria [12,13]. These techniques allow for the confirmation of identity of isolates, direct detection of gene sequences from the clinical specimens and also for molecular detection of drug resistance [12]. Many previous publications have shown the sensitivity and specificity of several molecular detection assays such as BDProbeTec ET, (Becton Dickinson), COBAS AMPLICOR (Roche), Amplified M. tuberculosis Direct Test AMTDT (Gen Probe, USA) for identification of mycobacteria [9].…”

Section: Direct Detection Methodsmentioning

confidence: 99%

“…The use of nucleic-acid probe identification systems was a one step ahead in the rapid identification of mycobacterium species of M. tuberculosis complex, M. avium complex, M. avium, M. intracellulare, M. kansasii, and M. gordonae and also other nontuberculous mycobacteria (NTM) in culture because the result can be obtained after 2 hours [10,12]. But the sensitivity and specificity of this probe technology will only approximate 100% if there are more than 100 mycobacteria present in the sample, except for M. kansasii (87%) [12].…”

Section: Direct Detection Methodsmentioning

confidence: 99%

“…But the sensitivity and specificity of this probe technology will only approximate 100% if there are more than 100 mycobacteria present in the sample, except for M. kansasii (87%) [12]. Thus, these probes are not sensitive enough to be used directly in clinical specimens like sputum.…”

Section: Direct Detection Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Tuberculosis - Current Issues in Diagnosis and Management

Mahboub¹,

Vats²

2013

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Section: Direct Detection Methodsmentioning

confidence: 99%

Section: Direct Detection Methodsmentioning

confidence: 99%

Section: Direct Detection Methodsmentioning

confidence: 99%

Section: Direct Detection Methodsmentioning

confidence: 99%

Section: Direct Detection Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Tuberculosis - Current Issues in Diagnosis and Management

Mahboub¹,

Vats²

2013

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Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis

et al. 2023

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Accurate and appropriate extrapulmonary tuberculosis (EPTB) diagnosis remains challenging due to its paucibacillary nature, requirement of invasive collection procedures, and lack of sensitive tests. This study investigated the diagnostic performance of different methods for the diagnosis of EPTB. A total of 1340 EPTB specimens were collected from presumptive EPTB patients from four different hospitals between November 2015 and March 2017. The collected specimens were tested with AFB microscopy, culture, Xpert MTB/RIF assay (Xpert), and MTBDRplus assay. Among the 1340 EPTB specimens, 49 (3.66%), 141 (10.52%), 166 (12.39%), and 154 (11.49%) were positive in AFB microscopy, culture, Xpert MTB/RIF, and MTBDRplus assay, respectively. A total of 194 (14.9%) cases were found positive in at least one of these methods. Using culture as a reference standard, the sensitivity and specificity of AFB microscopy, Xpert MTB/RIF, and MTBDRplus assay were: 27.0%/99.1%, 83.7%/96.0%, and 79.4%/96.5%, respectively. Compared to the composite reference standard, the sensitivity of culture, AFB microscopy, Xpert MTB/RIF, and MTBDRplus assay was 72.7%, 25.3%, 85.6%, and 79.4%, respectively, with a specificity of 100% for all the methods. The Xpert MTB/RIF assay showed the highest sensitivity compared to other methods. Considering the short turnaround time and promising findings, Xpert MTB/RIF assay should be integrated into national TB guidelines as a routine diagnostic test.

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Diagnóstico de tuberculosis: desde lo tradicional hasta el desarrollo actual

et al. 2015

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La tuberculosis es un problema de salud pública que afecta a millones de personas, siendo la cuarta causa de muerte por enfermedad infecciosa en el mundo. En su más reciente reporte, la Organización Mundial de la Salud (OMS) menciona que la infección tuberculosa es curable con un tratamiento adecuado; sin embargo, es una necesidad la detección precoz de casos y la mejora del diagnóstico como medida de control. En esta revisión se presenta una recopilación de los métodos de detección de tuberculosis desde los tradicionales hasta las nuevas alternativas en desarrollo. Como métodos convencionales de diagnóstico para la detección de la tuberculosis se han usado el examen directo con coloración de esputo y el cultivo para determinar la presencia de la micobacteria; estos métodos presentan desventajas importantes que afectan la sensibilidad y tiempo para el diagnóstico. Recientes avances en pruebas rápidas incluyen el desarrollo de técnicas moleculares e inmunoensayos que pueden detectar micobacterias resistentes a antibióticos y anticuerpos de la respuesta inmune del hospedero. Sin embargo, esta enfermedad no cuenta con una prueba de diagnóstico precisa que permita la detección y el diagnóstico rápido con la mínima pérdida de pacientes. Frente a estos retos, en la actualidad se encuentran en desarrollo nuevas pruebas basadas en biosensores mediante el uso de biomarcadores adecuados de la enfermedad.

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Advances in Molecular Diagnosis of Tuberculosis

Cited by 6 publications

References 31 publications

Tuberculosis - Current Issues in Diagnosis and Management

Tuberculosis - Current Issues in Diagnosis and Management

Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis

Diagnóstico de tuberculosis: desde lo tradicional hasta el desarrollo actual

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