Advances in molecular methods to alter chromosomes and genome in the yeast Saccharomyces cerevisiae

Abstract

A fundamental issue in biotechnology is how to breed useful strains of microorganisms for efficient production of valuable biomaterials. On-going and more recent developments in gene manipulation technologies and chromosomal and genomic modifications in particular have facilitated important contributions in this area. "Chromosome manipulation technology" as an outgrowth of "gene manipulation technology" may provide opportunities for creating novel strains of organisms with a variety of genomic constitutions. A simple and rapid chromosome splitting technology called "PCR-mediated chromosome splitting" (PCS) that we recently developed has made it possible to manipulate chromosomes and genomes on a large scale in an industrially important microorganism, Saccharomyces cerevisiae. This paper focuses on recent advances in molecular methods for altering chromosomes and genome in S. cerevisiae featuring chromosome splitting technology. These advances in introducing large-scale genomic modifications are expected to accelerate the breeding of novel strains for biotechnological purposes, and to reveal functions of presently uncharacterized chromosomal regions in S. cerevisiae and other organisms.

Yeasts for Global Happiness: report of the 14th International Congress on Yeasts (ICY14) held in Awaji Island

“…C) regarding the frontier of genome engineering technologies for bioscience and biotechnology (Sugiyama et al . ; Natesuntorn et al . ) (day 1); (ii) ‘A multi‐faceted cellular response against aneuploidy stress’ by Dr. Davis Ng (National University of Singapore, Singapore; Fig.…”

“…The plenary lectures of ICY14 consisted of: (i) 'Yeast genome engineering -A new challenge to deciphering genome function and breeding' by Dr. Satoshi Harashima (Sojo University, Japan; Fig. 1C) regarding the frontier of genome engineering technologies for bioscience and biotechnology (Sugiyama et al 2009;Natesuntorn et al 2015) (day 1); (ii) 'A multifaceted cellular response against aneuploidy stress' by Dr. Davis Ng (National University of Singapore, Singapore; Fig. 1D) regarding biochemical and genomewide analyses of cellular responses induced by genomic imbalance from the perspective of proteotoxic stress (Thibault & Ng 2012) (day 2); (iii) 'Yeast as a model organism in ecology and evolution' by Dr. Graham Bell (McGill University, Fig.…”

A levedura como modelo para estudar as bases moleculares da doença de Parkinson

“…É também fácil de manipular geneticamente por ter uma elevada eficiência de transformação e uma eficiente via de recombinação homóloga que permitem eliminar ou mutar o seu genoma ou mesmo inserir ADN exógeno com elevada eficiência (SUGIYAMA et al, 2009). Foi devido a estas características que a levedura S. cerevisiae foi o primeiro organismo eucariótico a ter o seu genoma completamente sequenciado em 1996 (GOFFEAU et al, 1996).…”

Simple is good: yeast models of neurodegeneration

“…With a cell cycle that involves haploid and diploid forms, the study of lethal mutations in heterozygous diploids and recessive mutations in haploids is extremely simplified in yeast. Furthermore, classical genetic manipulations are facilitated by mating the haploid strains and sporulating diploid strains, while molecular genetics is aided by the high transformation efficiency of these cells and by the presence of a very efficient homologous recombination pathway, which renders it relatively easy to insert, delete or mutate any genomic sequence up to the chromosome level (Sugiyama et al , 2009).…”