Advances in Multiplexed Microfluidics for Infectious Disease Detection

Abstract

Microfluidics enables miniaturization, functionality, high throughput and reproducibility of multipathogen detection. Multiplexed microfluidic devices are electrochemical sensor-based, optical sensor-based, immunosensor-based and paper-based multiplexed microfluidics. However, the simultaneous detection of multiple pathogens is limited because of the complexity and diversity of infectious disease sources and mutual interference among analytes. This review provides an overview of recent advances in developing multiplex diagnostic microfluidic devices for detecting infectious diseases and discusses practical issues and perspectives. This review also covers microfluidic nucleic acid amplification strategies to improve detection sensitivity. Finally, we discuss the limitations and challenges in the design of multiplexed microfluidics.

Fate of Sniff-the-Diseases Through Nanomaterials-Supported Optical Biochip Sensors

“…Although optical sensors can identify infectious diseases in minutes using multiplex detection, they are only helpful when equipped with extensive data handling capabilities and particular fluorescent labels. 55 When combined with DNA, nanostructures made of fullerene and other carbon allotropes have great potential as new nanobiomaterials for use in biochips and sensitive biosensors. 56 Surface plasmon resonance sensors have been used for disease diagnosis, interaction mechanism studies, and safety detection applications.…”