Advances in musculoskeletal tissue engineering

Rossi, Claudia; Pozzobon, Michela; Coppi, Paolo De

doi:10.4161/org.6.3.12419

Cited by 97 publications

(83 citation statements)

References 64 publications

(57 reference statements)

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“…Engineered muscle constructs, composed of an adequate scaffold and autologous muscle cells represent a promising treatment for patients with either irreversible skeletal muscle damage or insufficient intrinsic muscle repair capacity (Koning et al 2009, Rossi et al 2010. The design of a tissue-specific scaffold, however, faces numerous challenges related to material choice and processing.…”

Section: Introductionmentioning

confidence: 99%

Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

et al. 2013

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Engineered muscle constructs provide a promising perspective on the regeneration or substitution of irreversibly damaged skeletal muscle. However, the highly ordered structure of native muscle tissue necessitates special consideration during scaffold development. Multiple approaches to the design of anisotropically structured substrates with grooved micropatterns or parallel-aligned fibres have previously been undertaken. In this study we report the guidance effect of a scaffold that combines both approaches, oriented fibres and a grooved topography. By electrospinning onto a topographically structured collector, matrices of parallel-oriented poly(ε-caprolactone) fibres with an imprinted wavy topography of 90 μm periodicity were produced. Matrices of randomly oriented fibres or parallel-oriented fibres without micropatterns served as controls. As previously shown, un-patterned, parallel-oriented substrates induced myotube orientation that is parallel to fibre direction. Interestingly, pattern addition induced an orientation of myotubes at an angle of 24• (statistical median) relative to fibre orientation. Myotube length was significantly increased on aligned micropatterned substrates in comparison to that on aligned substrates without pattern (436 ± 245 μm versus 365 ± 212 μm; p < 0.05). We report an innovative, yet simple, design to produce micropatterned electrospun scaffolds that induce an unexpected myotube orientation and an increase in myotube length.

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Section: Introductionmentioning

confidence: 99%

Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

et al. 2013

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“…However, the muscle tissue is not able to self-repair after significant tissue loss due to tumor ablation, injury, congenital defects, or prolonged denervation. 1 In vitro tissue engineering represents an alternative for the replacement of damaged tissue, which is a current challenge in regenerative and translational medicine. In order to mimic the physiological architecture of skeletal muscle cells, the development of muscle tissue in vitro should be precisely controlled to distribute the critical signals throughout myogenesis.…”

Section: Introductionmentioning

confidence: 99%

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Monge

Ren

Berton

et al. 2012

Tissue Engineering Part A

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“…The ability to create engineered muscle tissues that mimic the structural, functional, and regenerative properties of native muscle would enable design of accurate in vitro models for studies of muscle physiology and development (3,4) and promote cell-based therapies for muscle injury and disease (5,6). Pioneering studies of Vandenburgh and coworkers (7) and Dennis and Kosnik (8) were the first to demonstrate in vitro engineering of functional mammalian muscle constructs, followed by other studies reporting that differentiated engineered muscle can survive and vascularize upon implantation in vivo (9)(10)(11)(12)(13).…”

mentioning

confidence: 99%

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Juhas

Engelmayr

Fontanella

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

216

316

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Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.tissue engineering | contractile force | self-repair | angiogenesis | window chamber

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Advances in musculoskeletal tissue engineering

Cited by 97 publications

References 64 publications

Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

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