Advances in nonlinear optical microscopy for biophotonics

Li, Rui; Wang, Xinxin; Zhou, Yi; Zong, Huan; Chen, Maodu; Sun, Mengtao

doi:10.1117/1.jnp.12.033007

Cited by 30 publications

(22 citation statements)

References 62 publications

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“…In nonlinear microscopy, high-energy pulses are emitted by a laser source with a low average energy provided to the sample, which reduces the chances of phototoxicity. In this process, the laser light excites the molecule, and its relaxation to ground state produces the fluorescence emission of light [25].…”

Section: Nonlinear Microscopymentioning

confidence: 99%

“…Similarly, two-photon microscopes are used for investigating the biological functions and disorders in tumor cells [28] and to image the neural tissues of the brain [29]. Some of the multiphoton-based microscopes presented in the literature are listed by Li et al in [25].…”

Section: Multiphoton Microscopymentioning

confidence: 99%

“…This kind of microscopy is mainly used to image collagen fibers, myosin in muscle fibers, and microtubules in axons because of their ordered tissue structures [25]. Due to the similarity of SHG and TPEF, these techniques are often used together.…”

Section: Higher Harmonic Generation Microscopymentioning

confidence: 99%

“…The molecule is unstable at that state and returns to its ground level with higher energy given by the sum of energies of the two beams. In CARS microscopy, this scattered photon is used for imaging the tissue structure [25].…”

Section: Raman Scattering Microscopymentioning

confidence: 99%

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Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art

2020

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The growth and development of optical components and, in particular, the miniaturization of micro-electro-mechanical systems (MEMSs), has motivated and enabled researchers to design smaller and smaller endoscopes. The overarching goal of this work has been to image smaller previously inaccessible luminal organs in real time, at high resolution, in a minimally invasive manner that does not compromise the comfort of the subject, nor introduce additional risk. Thus, an initial diagnosis can be made, or a small precancerous lesion may be detected, in a small-diameter luminal organ that would not have otherwise been possible. Continuous advancement in the field has enabled a wide range of optical scanners. Different scanning techniques, working principles, and the applications of endoscopic scanners are summarized in this review.

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Section: Nonlinear Microscopymentioning

confidence: 99%

Section: Multiphoton Microscopymentioning

confidence: 99%

Section: Higher Harmonic Generation Microscopymentioning

confidence: 99%

Section: Raman Scattering Microscopymentioning

confidence: 99%

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Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art

2020

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“… TPEF can be used to investigate elastic fibers and some cell metabolic compounds. Several nonlinear interactions of SHG, CARS and TPEF occur only in very specific intrinsic biological molecules. This allows for imaging without the need for exogenous stains, which is very convenient for in vivo applications.…”

Section: Introductionmentioning

confidence: 99%

Biological nascent evolution of snail bone and collagen revealed by nonlinear optical microscopy

Wang

et al. 2019

Journal of Biophotonics

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Using nonlinear optical microscopy of coherent antistokes Raman scattering (CARS), second harmonic generation (SHG) and two-photo excitation fluorescence, we in situ observed how the collagen and the bone grow synergistically and competitively during nascent biological evolution. The sdsCO 2− 3 and PO 2− 3 ions were first observed to be dispersed in the liquid environment, and the collagen was observed 2 days later. With the help of the collagen, the CO 2− 3 and PO 2− 3 ions gradually moved closer to the collagen, and then the bone was produced in the forms of CaCO 3 and CaPO 3 . When the bone was completed with the help of the collagen, the collagen gradually disappeared. The biological evolution of snail bone and collagen can be well revealed by CARS and SHG, and in addition, the biological evolution of structure and morphology can be clearly observed day by day. K E Y W O R D S biological nascent evolution, bone, CARS, collagen, nonlinear optical microscopy, SHG, snail, TPEF

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Types of spectroscopy and microscopy techniques for cancer diagnosis: a review

et al. 2022

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Cancer is a life-threatening disease that has claimed the lives of many people worldwide. With the current diagnostic methods, it is hard to determine cancer at an early stage, due to its versatile nature and lack of genomic biomarkers. The rapid development of biophotonics has emerged as a potential tool in cancer detection and diagnosis. Using the fluorescence, scattering, and absorption characteristics of cells and tissues, it is possible to detect cancer at an early stage. The diagnostic techniques addressed in this review are highly sensitive to the chemical and morphological changes in the cell and tissue during disease progression. These changes alter the fluorescence signal of the cell/tissue and are detected using spectroscopy and microscopy techniques including confocal and two-photon fluorescence (TPF). Further, second harmonic generation (SHG) microscopy reveals the morphological changes that occurred in non-centrosymmetric structures in the tissue, such as collagen. Again, Raman spectroscopy is a non-destructive method that provides a fingerprinting technique to differentiate benign and malignant tissue based on Raman signal. Photoacoustic microscopy and spectroscopy of tissue allow molecule-specific detection with high spatial resolution and penetration depth. In addition, terahertz spectroscopic studies reveal the variation of tissue water content during disease progression. In this review, we address the applications of spectroscopic and microscopic techniques for cancer detection based on the optical properties of the tissue. The discussed state-of-the-art techniques successfully determines malignancy to its rapid diagnosis.

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Advances in nonlinear optical microscopy for biophotonics

Cited by 30 publications

References 62 publications

Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art

Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art

Biological nascent evolution of snail bone and collagen revealed by nonlinear optical microscopy

Types of spectroscopy and microscopy techniques for cancer diagnosis: a review

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