Advances in Plant Based Biologics

Gowtham, Iyappan; Ramalingam, Sathishkumar

doi:10.1021/bk-2019-1329.ch004

Cited by 1 publication

(1 citation statement)

References 184 publications

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“…Since more than two decades, several plant viruses attract increasing attention also from a different point of view: Their precise and robust nanostructures with repetitively organized, multivalent protein surfaces lend these viruses and derivatives thereof to uses in medical and technical environments, as carrier particles for the delivery and/or display of functional units enclosed and/or exposed at high densities (Bittner et al, 2013;Lin and Ratna, 2014;Culver et al, 2015;Khudyakov and Pumpens, 2016;Koch et al, 2016;Wen and Steinmetz, 2016;Dragnea, 2017;Steele et al, 2017;Lomonossoff and Wege, 2018;Wege and Lomonossoff, 2018;Balke and Zeltins, 2019;Chen et al, 2019;Eiben et al, 2019;Roeder et al, 2019;Chung et al, 2020;Wege and Koch, 2020;Wen et al, 2020). The respective plant viruses and VLPs are richly and sustainably available by farming (Marsian and Lomonossoff, 2016;Gowtham and Sathishkumar, 2019;Rybicki, 2020), and despite a remarkable durability biodegradable after use. Certain plant viral CPs are amenable to modifications facilitating the selective coupling of functional molecules, and to in vivo or in vitro assembly into VLPs even in the absence of viral nucleic acids (Wege and Koch, 2020).…”

Section: Plant Virus-based Building Blocks Enhancing Biosensor Performentioning

confidence: 99%

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

Poghossian¹,

Jablonski

Molinnus

et al. 2020

Front. Plant Sci.

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Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.

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