Advances in recombinant protease production: current state and perspectives

Liu, Xiufang; Lian, Mulin; Zhao, Mouming; Huang, Mingtao

doi:10.1007/s11274-024-03957-5

World J Microbiol Biotechnol

2024

DOI: 10.1007/s11274-024-03957-5

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Advances in recombinant protease production: current state and perspectives

Xiufang Liu,

Mulin Lian,

Mouming Zhao

et al.

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2024

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Cited by 4 publications

References 161 publications

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Cloning and expression of recombinant arazyme with anti-inflammatory and anti-breast cancer potential

Yahyaee,

Shahpari,

Mousavi Ghahfarrokhi

et al. 2024

Arch Microbiol

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Cloning and expression of recombinant arazyme with anti-inflammatory and anti-breast cancer potential

Yahyaee,

Shahpari,

Mousavi Ghahfarrokhi

et al. 2024

Arch Microbiol

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Comprehensive Understanding of Laboratory Evolution for Food Enzymes: From Design to Screening Innovations

Wu,

Wang,

Zeng

et al. 2024

J. Agric. Food Chem.

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In the field of food processing, enzymes play a pivotal role in improving product quality and flavor, and extending shelf life. However, the exposure of traditional food enzymes to high temperatures during processing often leads to a decrease in activity or even inactivation, limiting the effectiveness of their application under high-temperature conditions. Therefore, the modification of thermostability and activity of enzymes to adapt to extreme conditions through protein engineering has become a key way to improve the efficiency and economic benefits of industrial production. Directed evolution and semirational design strategies in the laboratory have proven to be broadly applicable frameworks for biochemical researchers in the food field, including those who are beginners. In this review, we systematically summarize semirational design strategies and high-throughput screening strategies, and introduce some intuitive computer simulation software to improve the thermostability and enzyme activity of food enzymes. The application of these strategies and techniques provides a comprehensive guide for the optimization of food enzymes. In addition, the latest hot topics of genetically engineered food enzymes in the field of application are discussed.

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Selective Hydrolysis of Heterooligosaccharides by Poly(acrylate) Gel Catalysts

Striegler

2024

ACS Catal.

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Natural glycoside hydrolases are distinguished by their ability to hydrolyze glycosidic bonds with high efficiency and selectivity. This feature is achieved through specific interactions in the active site during catalytic turnover and is not just facilitated by two catalytically active amino acids. Intrigued by these features, a biomimetic α-galactosidase mimic was developed using an empirical catalyst design. Starting with a library of 704 gels of which 250 have a unique composition synthesized from TEGDMA cross-linker and 7 selected monomers, 238 monomodal gels are evaluated for their ability to hydrolyze the 1→6 αglycosidic bond in the disaccharide melibiose. Among those, 13 polyacrylate gels with the potential for high catalytic activity are identified using spectrophotometric screening assays based on Schiff bases formed with toluidine. The best-performing polyacrylate (gel A) was found to have a 1500-fold higher proficiency to hydrolyze the 1→6 αglycosidic bond in melibiose over the 1→2 α-glycosidic bond in sucrose, translating to selective hydrolysis of the glycosidic linkages in the trisaccharide raffinose. The matrix of gel A is composed of 25 mol % TEGDMA cross-linker and equimolar amounts of cyclohexyl, butyl, and benzyl acrylate accounting for CH-π and hydrophobic interaction in the surrounding of a hydrolytic binuclear Cu(II) complex. The combined observations underline a paramount influence of matrix-stabilizing effects on the transition state of the hydrolysis of glycosidic bonds and may pave the way for the rapid development of catalysts transforming biomass.

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Advances in recombinant protease production: current state and perspectives

Cited by 4 publications

References 161 publications

Cloning and expression of recombinant arazyme with anti-inflammatory and anti-breast cancer potential

Cloning and expression of recombinant arazyme with anti-inflammatory and anti-breast cancer potential

Comprehensive Understanding of Laboratory Evolution for Food Enzymes: From Design to Screening Innovations

Selective Hydrolysis of Heterooligosaccharides by Poly(acrylate) Gel Catalysts

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