Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Fu, Weilai; Song, Guolong; Wang, Yunshuang; Wang, Qiang; Duan, Peifeng; Liu, Chao; Zhang, Xian; Rao, Zhiming

doi:10.3389/fenvs.2022.887093

Cited by 21 publications

(6 citation statements)

References 138 publications

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“…The NH4 + -N removals of K3 and S3 were promoted by 26.53% and 16.61% compared to CK, respectively. These results were consistent with the findings of a summer study [31], indicating that the addition of biochar to the ecological ditch at low temperature also had a significant improvement on NH4 + -N removal. It is widely recognized that DO is the key factor affecting NH4 + -N removal; studies have shown that aeration increased DO to improve the NH4 + -N removal in constructed wetlands [32,33].…”

Section: N and P Removal By Each Groupsupporting

confidence: 91%

“…Except for the first day, the concentrations in all groups of mixed additions were smaller than the concentrations in the treatment groups corresponding to the CK and rhizosphere addition groups (S1, S2, and S3). The accumulation of NO 2 − -N was mainly due to the lack of an organic carbon source, but FU et al proposed that the nitrification rate was greater than the denitrification rate in a high NH 4 + -N concentration, which was due to the DO limitation [31]. Due to the photosynthesis of V. natans, the treatment groups in this experiment maintained a satisfactory DO level.…”

Section: N and P Removal By Each Groupmentioning

confidence: 63%

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Added Biochars Promoted Nitrogen and Phosphorus Removal from Ecological Ditches at Low Temperature

Bai,

Pang,

et al. 2024

Water

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The global issue of ecological ditches being poor in removing nitrogen and phosphorus under cold winter temperatures has been identified. This study introduced three types of biochar (reed, rice, and corn) into ecological ditch sediments via two application methods: rhizosphere and mixed addition. The purpose was to explore how these methods affect the removal of nitrogen and phosphorus, as well as their influences on microbial communities in sediments. The results indicated that the addition of biochar to ecological ditches significantly enhanced the removal of nitrogen and phosphorus. Among the three types of biochar, the mixed addition of corn biochar yielded the greatest results, achieving removal rates of 77.1% for total nitrogen (TN), 93.3% for NH4+-N, and 90.3% for total phosphorus (TP). The growth of Vallisneria natans was greatly improved by the mixed addition method, resulting in an average increase of 154%. This improvement was superior to the rhizosphere addition group, which led to a growth increase of 125%. In comparison, the control group (CK) showed a decrease of 4.8% in growth. Different methods of biochar addition resulted in changes in the physicochemical properties and stoichiometry of the plants. Microbial analyses showed that the addition of biochar reduced the diversity and abundance of the substrate microbial community.

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Section: N and P Removal By Each Groupsupporting

confidence: 91%

Section: N and P Removal By Each Groupmentioning

confidence: 63%

Added Biochars Promoted Nitrogen and Phosphorus Removal from Ecological Ditches at Low Temperature

Bai,

Pang,

et al. 2024

Water

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“…Nitri cation and denitri cation are the two most well-understood processes for microbiological nitrogen processing. Many novel heterotrophic aerobic denitri ers can perform nitri cation too in the mesophilic condition and within a neutral pH (Rajta et al, 2020;Fu et al, 2022). Heterotrophic denitri ers degrade organic compounds for cell growth and release inorganic molecules.…”

Section: Genome-wide Denitri Cation Nitrate Dissimilation and Ammoniu...mentioning

confidence: 99%

“…The nasTS and nasABGHC gene clusters that encode for the NAS were annotated in P. DMF. However, the absence of a genetic module for the ammonium nitri cation pathway separates P. DMF from well-understood heterotrophic nitrifying and aerobic denitrifying microorganisms (HNADs)(Fu et al, 2022). The conventional ammonium nitri cation pathway also contains ammonia monooxygenase (AMO; DMF, the ammonium assimilation pathway to x ammonia in the form of glutamate occurs via two pathways(Goss et al, 2001).…”

mentioning

confidence: 99%

Insight into the metabolic pathways of Paracoccus sp. strain DMF: a non-marine halotolerant methylotroph capable of degrading aliphatic amines/amides

Arya,

Maurya,

Ramanathan

2023

Environ Sci Pollut Res

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Paracoccus sp. strain DMF (P. DMF from henceforth) is a gram-negative heterotroph known to tolerate and utilize high concentrations of N, N-dimethylformamide (DMF). The work presented here elaborates on the metabolic pathways involved in the degradation of C1 compounds, many of which are well-known pollutants and toxic to the environment. Investigations on microbial growth and detection of metabolic intermediates corroborate the outcome of the functional genome analysis. Several classes of C1 compounds, such as methanol, methylated amines, aliphatic amides, and naturally occurring quaternary amines like glycine betaine, were tested as growth substrates. The detailed growth and kinetic parameter analyses reveal that P. DMF can e ciently aerobically degrade trimethylamine (TMA) and grow on quaternary amines such as glycine betaine. The results show that the mechanism for halotolerant adaptation in the presence of glycine betaine is dissimilar from those observed for conventional trehalose-mediated halotolerance in heterotrophic bacteria. In addition, a close genomic survey revealed the presence of a Co(I)-based substrate-speci c corrinoid methyltransferase operon, referred to as mtgBC. This demethylation system has been associated with glycine betaine catabolism in anaerobic methanogens and is unknown in denitrifying aerobic heterotrophs. This report on an anoxic-speci c demethylation system in an aerobic heterotroph is unique. Our nding exposes the metabolic potential for the degradation of a variety of C1 compounds by P. DMF, making it a novel organism of choice for remediating a wide range of possible environmental contaminants.

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“…Bacillus (Rout et al, 2017), and Klebsiella (Li et al, 2019), were sequentially isolated from activated sludge, industrial wastewater, rice soils, etc. With the widespread attention on aerobic denitrifying bacteria, their denitrification characteristics including influence by nutritional and physical factors, metabolic pathways, and applications in practical engineering have been investigated (Fu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Isolation and nitrogen removal characteristics of a novel aerobic denitrifying strainAchromobacter xylosoxidansGR7397

Yao

et al. 2023

Preprint

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Aerobic denitrifying bacteria have the potential for engineering applications due to the efficient nitrate removal capacity from wastewater. In this study, a novel aerobic denitrifying strain was isolated and identified as Achromobacter xylosoxidans GR7397 from the activated sludge of a wastewater treatment plant, which possessed efficient nitrate removal capacity. Moreover, the denitrification capacity and properties of the strain were investigated in the presence of nitrate as the only nitrogen source. Five denitrification reductases encoding genes were harbored by strain GR7397 determined by electrophoretic analysis of PCR amplification products, consisting of periplasmic nitrate reductase (NAP), nitrate reductase (NAR), nitrite reductase (NIR), nitrous oxide reductase (NOS), and nitric oxide reductase (NOR), demonstrating that the strain has a complete denitrification metabolic pathway. The optimum denitrifying condition of strain GR7397 included sodium acetate adopted as the electron donor, COD/TN ratio at 4, pH at 8, temperature at 30 degrees C, under which condition, the nitrate removal rate reached 14.86 mg(L-1h-1) that the NO- 3-N concentration decreased from 93.90 mg/L to 4.73 mg/L within 6 h with no accumulation of nitrite. In addition, the bioaugmentation performance of strain GR7397 to enhance nitrate removal was evaluated to be effective and stabilized in a sequential batch reactor (SBR). The removal rate of NO- 3-N was the highest during each cycle with a range of 15.48-28.56 mg(L-1h-1) in the SBR with inoculating 30% of the strain concentrate. The current research demonstrated that strain GR7397 has significant potential for application in enhancing nitrogen removal in wastewater treatment.

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Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Cited by 21 publications

References 138 publications

Added Biochars Promoted Nitrogen and Phosphorus Removal from Ecological Ditches at Low Temperature

Added Biochars Promoted Nitrogen and Phosphorus Removal from Ecological Ditches at Low Temperature

Insight into the metabolic pathways of Paracoccus sp. strain DMF: a non-marine halotolerant methylotroph capable of degrading aliphatic amines/amides

Isolation and nitrogen removal characteristics of a novel aerobic denitrifying strainAchromobacter xylosoxidansGR7397

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