Advances in Silver Ion Chromatography for the Analysis of Fatty Acids and Triacylglycerols—2001 to 2011

Chromatography with silver nitrate: part 2

“…14, 15 Momchilova et al 16,17 have been prolific in the area of fatty acid, lipid and triacylglycerol separation. Utilising SNIA Charkraborty 18 was able to purify eicosapentaenoic acid (10) derived from chemically hydrolyzed sardine oil (Figure 4).…”

“…For example, exposing all cis 13 to the thiyl radical and obtaining 14 after SNIS purification (Scheme 1). [20][21][22][23][24][25][26][27] M A N U S C R I P T Braun, 29 in a continuing study of Western Australian sandalwood oil, required SNIS to resolve three isomers of acoren-11-ol (16)(17)(18). In the same year Braun et al 30 described the use of SNIS to separately purify (Z)-γ-curcumen-l2-ol (19) and (Z)-β-curcumen-l2-ol (20), with the former requiring the SNIS to be stabilized with BHT to prevent oxidation of the conjugated diene.…”

Isolation of α-linolenic acid biohydrogenation products by combined silver ion solid phase extraction and semi-preparative high performance liquid chromatography

“…This is the first report of semipreparative collection of purified t12-t13-, t14, t15-and t16-18:1, allowing for future investigations into their bioactivity in cell culture. Previously, Ag + -HPLC or RF-HPLC has been mostly employed to collect fractions enriched with t-18:1 prior to GC analyses, but fractions contained multiple isomers [18,[20][21]27]. One RF-HPLC method has been reported allowing collection of individual c-17:1 isomers, however, limited resolution prevented similar collection of individual t-17:1 isomers [28].…”

“…Comprehensive analysis of BHP can also be complex, requiring multiple gas chromatographic (GC) analyses combined with silver ion high performance liquid chromatography (Ag + -HPLC) [16][17][18]. In addition, when analyzing ruminant products with complex fatty acid (FA) profiles, Ag + -thin layer chromatography (Ag + -TLC) or Ag + -solid phase extraction (Ag + -SPE)is sometimes employed to pre-fractionation 1-2 mg of fatty acid methyl esters (FAME) prior to GC analyses [16][17].…”

Separation of Enantiomeric Triacylglycerols by Chiral‐Phase HPLC

“…Separation of regioisomeric TAG has been described many times, beginning with the pioneering study [6] and including the review [7] describing their separations from tens of different oils and fats by non-aqueous reversed phaseliquid chromatography (NARP-LC) [8] and silver-LC [9]. The separation of regioisomeric TAG does therefore present no problems for chemists versed in this field.…”