Advances in Site-specific and Linkage-specific Ligation

Tam, James P.; Nguyen, Giang Kien Truc; Kam, Antony; Loo, Shining

doi:10.17952/35eps.2018.003

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Peptide Ligases and Enzyme Engineering To Enhance Catalytic ...1

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Cited by 2 publications

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“…For example. Tam et al 86 carried out labelling of a HER2-specific DARPin (designed ankyrin repeat protein), having a C-terminal NHV motif, with a fluorescent peptide containing an N-terminal RIGK motif for bioimaging as well as to ligate cytolytic peptides to generate candidate therapeutic drug conjugates.…”

Section: Peptide Ligases and Enzyme Engineering To Enhance Catalytic ...mentioning

confidence: 99%

Challenges in the use of sortase and other peptide ligases for site-specific protein modification

2022

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Section: Peptide Ligases and Enzyme Engineering To Enhance Catalytic ...mentioning

confidence: 99%

Challenges in the use of sortase and other peptide ligases for site-specific protein modification

2022

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Peptide asparaginyl ligases—renegade peptide bond makers

et al. 2020

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Making peptide bonds is tightly controlled by genetic code and machinery which includes cofactors, ATP, and RNAs. In this regard, the stand-alone and genetic-code-independent peptide ligases constitute a new family of renegade peptide-bond makers. A prime example is butelase-1, an Asn/Asp(Asx)-specific ligase that structurally belongs to the asparaginyl endopeptidase family. Butelase-1 specifically recognizes a C-terminal Asx-containing tripeptide motif, Asn/Asp-Xaa-Yaa (Xaa and Yaa are any amino acids), to form a site-specific Asn-Xaa peptide bond either intramolecularly as cyclic proteins or intermolecularly as modified proteins. Our work in the past five years has validated that butelase-1 is a potent and versatile ligase. Here we review the advances in ligases, with a focus on butelase-1, and their applications in engineering bioactive peptides and precision protein modifications, antibody-drug conjugates, and live-cell labeling.

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Advances in Site-specific and Linkage-specific Ligation

Cited by 2 publications

References 10 publications

Challenges in the use of sortase and other peptide ligases for site-specific protein modification

Challenges in the use of sortase and other peptide ligases for site-specific protein modification

Peptide asparaginyl ligases—renegade peptide bond makers

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