Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae

Xiao, Chufan; Pan, Yong; Huang, Mingtao

doi:10.1016/j.engmic.2023.100103

Cited by 10 publications

(5 citation statements)

References 106 publications

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“…Moreover, S. pastorianus exhibits significant upregulation in genes associated with the one-carbon metabolic process, transsulfuration, the ubiquinone biosynthetic process, protein folding and rRNA processing, endoplasmic reticulum membrane, glycolytic process, ATPase activity, protein folding, and unfolded protein binding. These genetic responses under the EsF system reflect the ability of the yeast to fine-tune its cellular machinery and biochemical pathways, resulting in changes in fermentation efficiency and metabolite production [26][27][28]. The coordinated regulation of these genes highlights the adaptive nature of the Saflager S-23 yeast in response to the EsF conditions.…”

Section: Discussionmentioning

confidence: 99%

“…While hundreds of aroma-active compounds have been found in beer, reviews tend to focus mainly on alcohols and esters produced by brewing [13], as in this work. Furthermore, the increase in NADH availability resulting from the enhanced expression of oxidoreductase activity genes in S. pastorianus leads to different patterns of redox homeostasis [28,34]. Thus, the metabolic networks, as observed in the different patterns of growth, substrate consumption, ethanol production, and volatile compounds using the EsF system.…”

Section: Discussionmentioning

confidence: 99%

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Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production

Amaro-Reyes,

Marcial-Ramírez,

Vázquez-Landaverde

et al. 2024

Foods

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Electrostatic fermentation avoids the cellular redox imbalance of traditional fermentation, but knowledge gaps exist. This study explores the impact of electrostatic fermentation on the growth, volatile profile, and genetic response of Saccharomyces pastorianus Saflager S-23. The applied voltage (15 and 30 V) in the electrostatic fermentation system increased the growth and substrate utilization of S. pastorianus while decreasing ethanol production. The aromas typically associated with traditional fermentation, such as alcoholic, grape, apple, and sweet notes, were diminished, while aromas like roses, fruits, flowers, and bananas were augmented in electrostatic fermentation. RNA-seq analysis revealed upregulation of genes involved in cell wall structure, oxidoreductase activity, and iron ion binding, while genes associated with protein synthesis, growth control, homeostasis, and membrane function were downregulated under the influence of applied voltage. The electrostatic fermentation system modulates genetic responses and metabolic pathways in yeast, rendering it a promising method for tailored beer production. Demonstrating feasibility under industrial-scale and realistic conditions is crucial for advancing towards commercialization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production

Amaro-Reyes,

Marcial-Ramírez,

Vázquez-Landaverde

et al. 2024

Foods

View full text Add to dashboard Cite

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“…Therefore, the full co-utilization of glucose and xylose represents a cost-effective approach for converting biomass feedstock into bioethanol [ 6 ]. Saccharomyces cerevisiae is an optimal cell factory due to its robust metabolic capacity and high tolerance to inhibitors [ [7] , [8] , [9] ]. However, it lacks the initial xylose metabolic pathway, and glucose use inhibits xylose utilization [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway

Qiu,

Liu,

et al. 2024

Synthetic and Systems Biotechnology

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“…Additionally, enhanced green fluorescent protein (EGFP) is used as a quantitative marker to measure expression intensity. The communication and response mediated by pheromones can be finely tuned based on the variety and amount of aromatic amino acids [20]. This study aims to improve Cordyceps' polysaccharide production efficiency, address limited natural resources, and gain insights into biosynthesis mechanisms, offering new avenues for the Cordyceps industry.…”

Section: Introductionmentioning

confidence: 99%

Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris

Qiao,

Chen,

Yang

2024

J Basic Microbiol

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This study aimed to enhance extracellular polysaccharide (EPS) production in Cordyceps militaris by constructing a quorum sensing (QS) system to regulate the expression of biosynthetic enzyme genes, including phosphoglucomutase, hexokinase, phosphomannomutase, polysaccharide synthase, and UDP‐glucose 4‐epimerase genes. The study found higher EPS concentrations in seven recombinant strains compared to the wild‐type C. militaris, indicating that the overexpression of key enzyme genes increased EPS production. Among them, the CM‐pgm‐2 strain exhibited the highest EPS production, reaching a concentration of 3.82 ± 0.26 g/L, which was 1.52 times higher than the amount produced by the wild C. militaris strain. Additionally, the regulatory effects of aromatic amino acids on the QS system of the CM‐pgm‐2 strain were investigated. Under the influence of 45 mg/L tryptophan, the EPS production in CM‐pgm‐2 reached 4.75 ± 0.20 g/L, representing a 1.90‐fold increase compared to wild C. militaris strains. This study provided an effective method for the large‐scale production of EPSs in C. militaris, and opened up new avenues for research into fungal QS mechanisms.

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Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae

Cited by 10 publications

References 106 publications

Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production

Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production

Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway

Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris

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