Advances in the Functional Nucleic Acid Biosensors for Detection of Lead Ions

Zhang, Buyue; Shi, Lei; Ma, Xiaoying; Liu, Lu; Fu, Yao; Zhang, Xiufeng

doi:10.1080/10408347.2021.1951648

Cited by 6 publications

(4 citation statements)

References 104 publications

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“…Deoxyribozyme (DNAzyme), recognized as an artificial catalytic oligonucleotide, is a short functional nucleic acid containing an active domain responsible for metal ion recognition. 10,11 Although DNAzyme-based assays have advantages in terms of high programmability and specificity, 12,13 their direct detection of Pb 2+ suffers from insufficient sensitivity, primarily due to the absence of adequate signal amplification. Therefore, developing effective signal amplification strategies is essential for advancing DNAzyme-based methods for detecting Pb 2+ .…”

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confidence: 99%

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Deng,

Bai,

Liu

et al. 2024

Chem. Commun.

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mentioning

confidence: 99%

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Deng,

Bai,

Liu

et al. 2024

Chem. Commun.

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“…High blood levels of Pb 2+ can seriously harm a human's health, especially in young kids. Children's blood serum contains abnormally high levels of Pb 2+ in a certain region which exceeds the recommended value of 1.00 µg/L [ 64 , 65 ]. Consequently, accurate Pb 2+ determination in biological and environmental materials is crucial.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals

Ibrahim,

Taha,

Hagaggi

et al. 2024

BMC Chemistry

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This study marks a pioneering effort in utilizing Vachellia tortilis subsp. raddiana (Savi) Kyal. & Boatwr., (commonly known as acacia raddiana) leaves as both a reducing and stabilizing agent in the green “eco-friendly” synthesis of silver nanoparticles (AgNPs). The research aimed to optimize the AgNPs synthesis process by investigating the influence of pH, temperature, extract volume, and contact time on both the reaction rate and the resulting AgNPs' morphology as well as discuss the potential of AgNPs in detecting some heavy metals. Various characterization methods, such as UV–vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), infrared spectroscopy (IR), Zeta sizer, EDAX, and transmitting electron microscopy (TEM), were used to thoroughly analyze the properties of the synthesized AgNPs. The XRD results verified the successful production of AgNPs with a crystallite size between 20 to 30 nm. SEM and TEM analyses revealed that the AgNPs are primarily spherical and rod-shaped, with sizes ranging from 8 to 41 nm. Significantly, the synthesis rate of AgNPs was notably higher in basic conditions (pH 10) at 70 °C. These results underscore the effectiveness of acacia raddiana as a source for sustainable AgNPs synthesis. The study also examined the AgNPs' ability to detect various heavy metal ions colorimetrically, including Hg2+, Cu2+, Pb2+, and Co2+. UV–Vis spectroscopy proved useful for this purpose. The color of AgNPs shifts from brownish-yellow to pale yellow, colorless, pale red, and reddish yellow when detecting Cu2+, Hg2+, Co2+, and Pb2+ ions, respectively. This change results in an alteration of the AgNPs' absorbance band, vanishing with Hg2+ and shifting from 423 to 352 nm, 438 nm, and 429 nm for Cu2+, Co2+, and Pb2+ ions, respectively. The AgNPs showed high sensitivity, with detection limits of 1.322 × 10–5 M, 1.37 × 10–7 M, 1.63 × 10–5 M, and 1.34 × 10–4 M for Hg2+, Cu2+, Pb2+, and Co2+, respectively. This study highlights the potential of using acacia raddiana for the eco-friendly synthesis of AgNPs and their effectiveness as environmental sensors for heavy metals, showcasing strong capabilities in colorimetric detection.

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“…Nanomaterials are also often combined with DNAzymes; among the most common scenarios is the use of nanomaterials as optical quenchers [ 40 ], as enhancers for the optical properties of respective sensing systems (e.g., the utilization of localized surface plasmon resonance (LSPR) characteristics of Au nanoparticles (NPs) [ 41 ], the use of quantum dots so as to achieve chemiluminescence resonance energy transfer (CRET) signals [ 42 ], or as appropriate platforms for DNA immobilization [ 43 ]. There are many articles and reviews [ 21 , 44 ] dedicated to the use of DNAzymes for Pb 2+ detection; the detection principle is again either colorimetric, fluorimetric, or electrochemical, while typical limits of detection (LoD) range between 0.5–400 nM, 100 pM–500 nM, and 6.4–910 pM for colorimetric, fluorescence, and electrochemical sensors, respectively. Typical LoDs in the case of Cd 2+ DNAzyme-based biosensors fall in the range of 1–11.3 nM [ 32 , 45 ]; in the case of a non-specific DNAzyme-based biosensor fabricated using a field-effect transistor (FET) and single-walled carbon nanotubes (SWNTs), the use of a mathematical model and data analysis results in a Cd 2+ LoD of 34 pM [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr3+

Skotadis,

Aslanidis,

Tsekenis

et al. 2023

Sensors

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A hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the detection of Pb2+, Cd2+, and Cr3+. The sensor takes advantage of a well-studied material that is known for its selective interaction with heavy metal ions (i.e., DNAzymes), which is combined with metallic nanoparticles. The double-helix structure of DNAzymes is known to dissociate into smaller fragments in the presence of specific heavy metal ions; this results in a measurable change in device resistance due to the collapse of conductive inter-nanoparticle DNAzyme bridging. The paper discusses the effect of DNAzyme anchoring groups (i.e., thiol and amino functionalization groups) on device performance and reports on the successful detection of all three target ions in concentrations that are well below their maximum permitted levels in tap water. While the use of DNAzymes for the detection of lead in particular and, to some extent, cadmium has been studied extensively, this is one of the few reports on the successful detection of chromium (III) via a sensor incorporating DNAzymes. The sensor showed great potential for its future integration in autonomous and remote sensing systems due to its low power characteristics, simple and cost-effective fabrication, and easy automation and measurement.

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Advances in the Functional Nucleic Acid Biosensors for Detection of Lead Ions

Cited by 6 publications

References 104 publications

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination

Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals

Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr3+

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