Advances in the management of recurrent or metastatic squamous cell carcinoma of the head and neck

Shin, Dong M.; Khuri, Fadlo R.

doi:10.1002/hed.21910

Cited by 37 publications

(35 citation statements)

References 149 publications

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“…Studies that only included OSCC in their evaluation show a comparably smaller number of HPV+ SCC, similar to this study [36, 37]. However, studies focusing on OPSCC, especially tonsil SCC, have higher HPV positivity rates [38] because the virus interacts with lymphoid tissue. The HPV has a selective tropism for the epithelium lining the tonsillar crypts.…”

Section: Discussionsupporting

confidence: 76%

Impact of HPV infection on oral squamous cell carcinoma

et al. 2016

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BackgroundHead and neck squamous cell carcinomas (HNSCC) are often divided by their aetiology. Noxae associated collectives are compared with the human papilloma virus (HPV)-associated group, whereas different localisations of oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinomas are mostly discussed as one single group. Our aim was to show that classification by aetiology is not appropriate for OSCC.ResultsHPV DNA was detected by PCR in 7 (3.47%) patients, and we identified 12 (5.94%) positive (+) cases by p16INK4a immunostaining. Only 4 (1.98%) of the p16INK4a+ cases were + for HPV using PCR. Our homogenous collective of OSCC allowed us to compare HPV+ and HPV negative (−) patients without creating bias for tumour localisation, age, gender or tumour stage.Materials and methodsAfter testing OSCC samples for HPV positivity, we compared the results of two commonly used HPV detection methods, p16INK4a immunostaining and HPV DNA-related PCR, on 202 OSCC patients. HPV subtypes were determined with an HPV LCD Array Kit. Clinicopathological features of the patients were analysed, and the disease specific survival rates (DSS) for HPV+ and HPV− patients were obtained.Conclusionsp16INK4a immunostaining is a not a reliable HPV detection method for OSCC. Positive p16INK4a immunostaining did not agree with + results from PCR of HPV DNA. Furthermore, the influence of HPV-related oncogenic transformation in OSCC is overestimated. The significance of HPV infection remains clinically unclear, and its influence on survival rates is not relevant to OSCC cases.

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Section: Discussionsupporting

confidence: 76%

Impact of HPV infection on oral squamous cell carcinoma

et al. 2016

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“…26 Some oral cancers may originate from precancerous lesions with epithelial dysplasia. 27,28 Previous studies also reported that oral of incubation, were assayed by MTT and LDH.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol

Tsai

Hsu

et al. 2016

Journal of the Formosan Medical Association

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“…2 Surgical management is usually the primary therapy for this disease, although radiation and chemotherapy also have prominent roles. 3 …”

Section: Introductionmentioning

confidence: 99%

Matrix‐Metalloproteinases in Head and Neck Carcinoma–Cancer Genome Atlas Analysis and Fluorescence Imaging in Mice

Hauff

Raju

Orosco

et al. 2014

Otolaryngol.--head neck surg.

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Objective (1) Obtain matrix-metalloproteinase (MMP) expression profiles for head and neck squamous cell carcinoma (HNSCC) specimens from the Cancer Genomic Atlas (TCGA). (2) Demonstrate HNSCC imaging using MMP-cleavable, fluorescently labeled ratiometric activatable cell-penetrating peptide (RACPP). Study Design Retrospective human cohort study; prospective animal study. Setting Translational research laboratory. Subjects and Methods Patient clinical data and mRNA expression levels of MMP genes were downloaded from TCGA data portal. RACPP provides complementary ratiometric fluorescent contrast (increased Cy5 and decreased Cy7 intensities) when cleaved by MMP2/9. HNSCC–tumor bearing mice were imaged in vivo after RACPP injection. Histology was evaluated by a pathologist blinded to experimental conditions. Zymography confirmed MMP-2/9 activity in xenografts. RACPP was applied to homogenized human HNSCC specimens, and ratiometric fluorescent signal was measured on a microplate reader for ex vivo analysis. Results Expression of multiple MMPs including MMP2/9 is greater in patient HNSCC tumors than matched control tissue. In patients with human papilloma virus positive (HPV+) tumors, higher MMP2 and MMP14 expression correlates with worse 5-year survival. Orthotopic tongue HNSCC xenografts showed excellent ratiometric fluorescent labeling with MMP2/9-cleavable RACPP (sensitivity = 95.4%, specificity = 95.0%). Fluorescence ratios were greater in areas of higher tumor burden (P <.03), which is useful for intraoperative margin assessment. Ex vivo, human HNSCC specimens showed greater cleavage of RACPP when compared to control tissue (P = .009). Conclusions Human HNSCC tumors show increased mRNA expression of multiple MMPs including MMP2/9. We used RACPP, a ratiometric fluorescence assay of MMP2/9 activity, to show improved occult tumor identification and margin clearance. Ex vivo assays using RACPP in biopsy specimens may identify patients who will benefit from intraoperative RACPP use.

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Advances in the management of recurrent or metastatic squamous cell carcinoma of the head and neck

Cited by 37 publications

References 149 publications

Impact of HPV infection on oral squamous cell carcinoma

Impact of HPV infection on oral squamous cell carcinoma

Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol

Matrix‐Metalloproteinases in Head and Neck Carcinoma–Cancer Genome Atlas Analysis and Fluorescence Imaging in Mice

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