Advances in the Researches of the Middle and Upper Atmosphere in China in 2020–2022

Zey, Chen

doi:10.11728/cjss2022.04.yg20

Cited by 1 publication

(1 citation statement)

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“…Moreover, several new CSC marker genes we identified in LSCC, including DMBT1 and SOX4. These new CSC marker genes have been previously reported to play significant roles in SCs development [36,37]. Utilizing public datasets and established CSC marker genes, we confirmed the presence of CSCs in LSCC.…”

Section: Discussionsupporting

confidence: 68%

Characterization of Cancer Stem Cells in Laryngeal Squamous Cell Carcinoma by Single-Cell RNA Sequencing

Li,

Lin,

Chu

et al. 2024

Preprint

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Cancer stem cells (CSCs) constitute a pivotal element within the tumor microenvironment (TME), driving the initiation and progression of cancer. However, the identification of CSCs and their underlying molecular mechanisms in laryngeal squamous cell carcinoma (LSCC) remains a formidable challenge. We employed single-cell RNA sequencing of matched primary tumor tissues, paracancerous tissues, and local lymph nodes from three LSCC patients. Two distinct clusters of stem cells originating from epithelial populations were delineated and verified as CSCs and normal stem cells (NSCs) respectively. CSCs were abundant in the paracancerous tissues compared to the tumor tissues. CSCs showed high expression of stem cell marker genes such as PROM1, ALDH1A1, and SOX4, and increased activity of tumor-related hypoxia, Wnt/β-catenin, and notch signaling pathways. We then explored the intricate crosstalk between CSCs and the TME cells and identified targets within the TME that related with CSCs. We also find eight marker genes of CSCs that correlated significantly with the prognosis of LSCC patients. Furthermore, bioinformatics analyses showed that drugs such as erlotinib, OSI-027, and ibrutinib selectively targeted the CSC-specifically expressed genes. In conclusion, our results represent the first comprehensive characterization of CSCs properties in LSCC at the single-cell level.

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Section: Discussionsupporting

confidence: 68%