Advances in the treatment of pediatric solid tumors: A 50‐year perspective

LaQuaglia, Michael P.; Gerstle, Justin T.

doi:10.1002/jso.27038

Cited by 5 publications

(3 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rhabdomyosarcoma (RMS), occurring in striated muscles throughout the body, is the most frequent soft tissue tumor in children [ 1 , 2 ]. RMS is classified into embryonal, alveolar, pleomorphic, and spindle-cell/sclerosing subtypes.…”

Section: Introductionmentioning

confidence: 99%

“…These mutations in muscular lineages, such as mesenchymal stem cells, skeletal muscle stem cells (satellite cells), or myogenic precursor cells (myoblasts), cause ERMS to present impaired myogenic differentiation and hyper-activated proliferation [ 6 , 7 , 8 ]. Alveolar RMS has been known to associate with the chromosomal translocations on PAX3 and PAX7 genes encoding Pax3/7 myogenic transcription factors [ 1 , 2 ]. Spindle-cell/sclerosing RMS has RAB3IP-HMGA2 fusion transcript and FGFR4 mutation [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells

et al. 2022

View full text Add to dashboard Cite

Embryonal rhabdomyosarcoma (ERMS) is the muscle-derived tumor retaining myogenic ability. iSN04 and AS1411, which are myogenetic oligodeoxynucleotides (myoDNs) serving as anti-nucleolin aptamers, have been reported to inhibit the proliferation and induce the differentiation of myoblasts. The present study investigated the effects of iSN04 and AS1411 in vitro on the growth of multiple patient-derived ERMS cell lines, ERMS1, KYM1, and RD. RT-PCR and immunostaining revealed that nucleolin was abundantly expressed and localized in nucleoplasm and nucleoli in all ERMS cell lines, similar to myoblasts. Both iSN04 and AS1411 at final concentrations of 10–30 μM significantly decreased the number of all ERMS cells; however, their optimal conditions were different among the cell lines. In all ERMS cell lines, iSN04 at a final concentration of 10 μM markedly reduced the ratio of EdU+ cells, indicating the inhibition of cell proliferation. Quantitative RT-PCR or immunostaining of phosphorylated histone H3 and myosin heavy chain demonstrated that iSN04 suppressed the cell cycle and partially promoted myogenesis but did not induce apoptosis in ERMS cells. Finally, both iSN04 and AS1411 at final concentrations of 10–30 μM disrupted the formation and outgrowth of RD tumorspheres in three-dimensional culture mimicking in vivo tumorigenesis. In conclusion, ERMS cells expressed nucleolin, and their growth was inhibited by the anti-nucleolin aptamers, iSN04 and AS1411, which modulates several cell cycle-related and myogenic gene expression. The present study provides evidence that anti-nucleolin aptamers can be used as nucleic acid drugs for chemotherapy against ERMS.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Chemerin was reported to inhibit differentiation and to enhance cell proliferation of C2C12 myoblasts, which is mediated through the extracellular-signal-regulated kinase (ERK)-1/2 and mammalian target of rapamycin (mTOR) signaling pathways [ 12 ]. Impaired muscle cell differentiation and accelerated cell proliferation are characteristics of rhabdomyosarcoma (RMS), a malignancy of the muscle which most commonly develops in children [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Loss of Chemerin in Rhabdomyosarcoma Cells Polarizes Adjacent Monocytes to an Immunosuppressive Phenotype

et al. 2022

View full text Add to dashboard Cite

Chemerin is a multifunctional adipokine that regulates adipogenesis, insulin signaling and blood pressure and has thus a central function in metabolism. Mounting evidence confirmed a function of chemerin in various cancers. In this study, we investigated the role of chemerin in rhabdomyosarcoma (RMS), an aggressive soft tissue cancer that affects mainly children and young adults. We found chemerin expression in 93.8% (90 of 96) of RMS cases, with a range of 86.7–96.7% for the four RMS subgroups. While chemerin is uniformly expressed in normal skeletal muscle, its expression in RMS is patchy with interspersed areas that are devoid of chemerin. This variable chemerin expression is reflected by RMS cell lines as two of them (Rh41 and Rd18) were found to secrete chemerin while the two other ones (JR1 and RD) were negative. Deletion of chemerin in Rh41 and Rd18 cells did not alter their growth rate or morphology. We investigated the potential influence of chemerin on immune surveillance by coculturing parental and chemerin-deficient RMS cells with resting- or lipopolysaccharide (LPS)-activated human peripheral monocytes. The absence of chemerin in the RMS cells led to increased expression levels of the coinhibitory molecules PD-L1 and PD-L2 while levels of the costimulatory molecule CD86 were not changed. Further, the absence of chemerin enhanced the secretion of cytokines (IL-1β, IL-6, IL-10 and TNF) that have been shown to support RMS pathogenesis. These data indicate that the loss of chemerin expression by RMS cells repolarizes monocytes in the tumor microenvironment to supporting tumor progression.

show abstract

Digital Pathology and Artificial Intelligence for Early Diagnosis of Pediatric Solid Tumors: Implication for Improved Healthcare Strategies

Shaterian,

Jandaghian-Bidgoli,

Shaterian

et al. 2024

Interdisciplinary Cancer Research

View full text Add to dashboard Cite

Advances in the treatment of pediatric solid tumors: A 50‐year perspective

Cited by 5 publications

References 101 publications

Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells

Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells

Loss of Chemerin in Rhabdomyosarcoma Cells Polarizes Adjacent Monocytes to an Immunosuppressive Phenotype

Digital Pathology and Artificial Intelligence for Early Diagnosis of Pediatric Solid Tumors: Implication for Improved Healthcare Strategies

Contact Info

Product

Resources

About