Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

He, Ziyu; Liu, Changsheng; Li, Zhongyu; Zhou, Chi; Chen, Xiang; Chen, Xupeng; Guo, Yuan

doi:10.3389/fbioe.2022.1020444

Cited by 3 publications

(2 citation statements)

References 113 publications

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“…Various preliminary findings collectively provide evidence that oral and endodontic bacteria can use mononuclear cells as Trojan horses to travel from their natural habitats to the systemic circulation and reach distant organs (Bordagaray et al., 2021; Garrido et al., 2019; Hazzah et al., 2015; Rodríguez‐Laiz et al., 2019). Poor intracellular permeability, degradation by an acidic, redox or multi‐enzymatic milieu, or discharge by efflux pumps result in poor intracellular antibacterial activity of antibiotics (Chen et al., 2023; Wright, 2005). AMPs represent a new potential alternative to be considered for fighting intracellular bacterial infections (Silva & Gomes, 2017).…”

Section: Amps In Endodontic Treatmentsmentioning

confidence: 99%

Harnessing antimicrobial peptides in endodontics

Kong,

Vishwanath,

Neelakantan

et al. 2024

Int Endodontic J

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Endodontic therapy includes various procedures such as vital pulp therapy, root canal treatment and retreatment, surgical endodontic treatment and regenerative endodontic procedures. Disinfection and tissue repair are crucial for the success of these therapies, necessitating the development of therapeutics that can effectively target microbiota, eliminate biofilms, modulate inflammation and promote tissue repair. However, no current endodontic agents can achieve these goals. Antimicrobial peptides (AMPs), which are sequences of amino acids, have gained attention due to their unique advantages, including reduced susceptibility to drug resistance, broad‐spectrum antibacterial properties and the ability to modulate the immune response of the organism effectively. This review systematically discusses the structure, mechanisms of action, novel designs and limitations of AMPs. Additionally, it highlights the efforts made by researchers to overcome peptide shortcomings and emphasizes the potential applications of AMPs in endodontic treatments.

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Section: Amps In Endodontic Treatmentsmentioning

confidence: 99%

Harnessing antimicrobial peptides in endodontics

Kong,

Vishwanath,

Neelakantan

et al. 2024

Int Endodontic J

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“…The analysis of genetic material can provide valuable information for identifying the pathogen type and providing targeted treatment. − Due to the high similarity between the different variants of the same pathogenic species, high specificity is required for bacterial subtyping techniques . Besides the widely used polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and DNA microarray assays, numerous novel nucleic acid detection methods have been developed using nanomaterials and enzymes. Examples include clustered regularly interspaced short palindromic repeats (CRISPR)-associated nucleases .…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Nanopore-Based Nucleic Acid Sensing and Bacterial Identification Using DNA Dumbbell Nanoswitches

et al. 2023

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Multiplexed nucleic acid sensing methods with high specificity are vital for clinical diagnostics and infectious disease control, especially in the postpandemic era. Nanopore sensing techniques have developed in the past two decades, offering versatile tools for biosensing while enabling highly sensitive analyte measurements at the single-molecule level. Here, we establish a nanopore sensor based on DNA dumbbell nanoswitches for multiplexed nucleic acid detection and bacterial identification. The DNA nanotechnology-based sensor switches from an "open" into a "closed" state when a target strand hybridizes to two sequencespecific sensing overhangs. The loop in the DNA pulls two groups of dumbbells together. The change in topology results in an easily recognized peak in the current trace. Simultaneous detection of four different sequences was achieved by assembling four DNA dumbbell nanoswitches on one carrier. The high specificity of the dumbbell nanoswitch was verified by distinguishing single base variants in DNA and RNA targets using four barcoded carriers in multiplexed measurements. By combining multiple dumbbell nanoswitches with barcoded DNA carriers, we identified different bacterial species even with high sequence similarity by detecting strain specific 16S ribosomal RNA (rRNA) fragments.

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Recent Developments in Electrochemical Sensors for the Detection of Antibiotic-Resistant Bacteria

Madhu¹,

Ramasamy²,

Choi³

2022

Pharmaceuticals

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The development of efficient point-of-care (POC) diagnostic tools for detecting infectious diseases caused by destructive pathogens plays an important role in clinical and environmental monitoring. Nevertheless, evolving complex and inconsistent antibiotic-resistant species mire their drug efficacy. In this regard, substantial effort has been expended to develop electrochemical sensors, which have gained significant interest for advancing POC testing with rapid and accurate detection of resistant bacteria at a low cost compared to conventional phenotype methods. This review concentrates on the recent developments in electrochemical sensing techniques that have been applied to assess the diverse latent antibiotic resistances of pathogenic bacteria. It deliberates the prominence of biorecognition probes and tailor-made nanomaterials used in electrochemical antibiotic susceptibility testing (AST). In addition, the bimodal functional efficacy of nanomaterials that can serve as potential transducer electrodes and the antimicrobial agent was investigated to meet the current requirements in designing sensor module development. In the final section, we discuss the challenges with contemporary AST sensor techniques and extend the key ideas to meet the demands of the next POC electrochemical sensors and antibiotic design modules in the healthcare sector.

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Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

Cited by 3 publications

References 113 publications

Harnessing antimicrobial peptides in endodontics

Harnessing antimicrobial peptides in endodontics

Multiplexed Nanopore-Based Nucleic Acid Sensing and Bacterial Identification Using DNA Dumbbell Nanoswitches

Recent Developments in Electrochemical Sensors for the Detection of Antibiotic-Resistant Bacteria

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