Advances in three-dimensional reconstruction of the experimental spinal cord injury

Duerstock, Bradley S.; Bajaj, Chandrajit L.; Pascucci, Valerio; Schikore, Daniel R.; Lin, Kwun-Nan; Borgens, Richard B.

doi:10.1016/s0895-6111(00)00034-3

Cited by 16 publications

(32 citation statements)

References 27 publications

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“…5 Spaces appear round to ovoid in shape. One of them is very close to a lymphatic vessel (positive for D2-40) present in the perilobular stroma reconstruction of normal structures and of specific lesions from the analysis of multiple histological sections (static 3D) has proven to be a valuable technique in different areas of pathology such as normal distribution of microcirculation of retina [14], para-trabecular pattern of infiltration of nonHodgkin's lymphoma in bone marrow [15], angiogenesis in the bone marrow of children with leukemia [16], distribution of microvessels in prostate cancer [17], evaluation and reconstruction of spinal cord injury [18], relationship between functional and structural changes of glomerular capillary networks in normal kidney [19], distribution and organisation of microvascular structure of cardiac coronary vascular tree [20], distribution of the vessels in normal and neoplastic thyroid [10] and distributions of vessels in oligodendrogliomas [21]. In breast, Ohtake et al [22,23], in a 3D reconstruction of intraductal extension of invasive breast carcinoma, have highlighted the presence of occasional intralobar and extralobar anastomoses which allowed the spread of in situ duct carcinoma through different lobes.…”

Section: Discussionmentioning

confidence: 99%

The pre-lymphatic pathway, the rooths of the lymphatic system in breast tissue: a 3D study

et al. 2008

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Three-dimensional (3D) visualisation of microscopic structures provides useful information about their configuration and the spatial (hence functional) relationship between different components in tissues. This paper describes 3D dynamic reconstructions of the pre-lymphatic labyrinth in two cases of "normal" breast core biopsies and one case of pseudoangiomatous stromal hyperplasia. Direct anastomoses between pre-lymphatic channels and true lymphatics of the breast were demonstrated. It is concluded that pre-lymphatics are a way of communication between breast epithelial/stromal structures and the main lymphatic system. The present findings suggest that the existence of pre-lymphatics has to be taken in consideration in the intramammary spread of malignant tumours.

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Section: Discussionmentioning

confidence: 99%

The pre-lymphatic pathway, the rooths of the lymphatic system in breast tissue: a 3D study

et al. 2008

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“…Registration was performed by aligning each histological section to be captured to the previous histological image. Anatomical features served as fiducial markers (Duerstock et al, 2000). The set of histological images were managed and three-dimensionally reconstructed on a Silicon Graphics ® Indigo computer (Mountain View, CA).…”

Section: Video Capturing and Registration Of Sections For 3d Visualizmentioning

confidence: 99%

“…Throughout each spinal cord data set, consecutive overlapping groups of three adjacent sections were averaged together to diminish any artifacts or histological defects present in a section. Averaging allowed only biological features that were consistent in two or more sections to be three-dimensionally reconstructed, thus eliminating any outlier histological defects from the 3D image (Duerstock et al, 2000).…”

Section: Three-dimensional Reconstructionmentioning

confidence: 99%

“…In neuroscience this has included; obscured regions of the brain (Halliday et al, 1993;Roesch et al, 1996), inner ear (Chimento et al, 1994;Hashimoto and Kimura, 1988), and the developing nervous system (Hounnou et al, 2003). In the field of neurotrauma, 3D reconstruction has been used to image the occult damage occurring in the central grey and white matter after contusion and compression injuries to the mammalian spinal cord (Beattie et al, 1997;Bresnahan et al, 1991;Duerstock and Borgens, 2002;Duerstock et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…3D surfaces are automatically rendered when the threshold of an anatomical feature is selected. Thus, no matter how complicated the histological structure(s), it can be easily imaged and measured (Duerstock et al, 2000). In the past, 3D surface reconstructions have been successfully used to compare the 3D shape and size of injury sites between blinded experimental and control groups of injured spinal cord (Duerstock and Borgens, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Double labeling serial sections to enhance three-dimensional imaging of injured spinal cord

Duerstock¹

2004

Journal of Neuroscience Methods

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Subcutaneous tri‐block copolymer produces recovery from spinal cord injury

Borgens

Bohnert

Duerstock

et al. 2004

J of Neuroscience Research

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We have studied the ability of nonionic detergents and hydrophilic polymers to seal permeabilized membranes of damaged cells, rescuing them from progressive dissolution, degeneration, and death. We report that a single subcutaneous injection of the tri-block copolymer, Poloxamer 188 (P188) 6 hr after a severe compression of the adult guinea pig spinal cord is able to: (1). preserve the anatomic integrity of the cord; (2). produce a rapid recovery of nerve impulse conduction through the lesion; and (3). produce a behavioral recovery of a spinal cord dependent long tract spinal cord reflex. These observations stood out against a control group in blinded evaluation. Conduction through the lesion was monitored by stimulating the tibial nerve of the hind limb, and measuring the arrival of evoked potentials at the contralateral sensory cortex of the brain (somatosensory evoked potentials; SSEP). Behavioral recovery was determined by a return of sensitivity of formerly areflexic receptive fields of the cutaneous trunchi muscle (CTM) reflex. This contraction of back skin in response to tactile stimulation is totally dependent on the integrity of an identified bilateral column of ascending long tract axons. A statistically significant recovery of both SSEP conduction through the lesion and the CTM reflex occurred in P188-treated animals compared to vehicle-treated controls. Quantitative 3D computer reconstruction of the lesioned vertebral segment of spinal cord revealed a statistically significant sparing of spinal cord parenchyma and a significant reduction in cavitation of the spinal cord compared to control animals We determined that the proportion of P188-treated animals that recovered evoked potentials were nearly identical to that produced by a subcutaneous injection of polyethylene glycol (PEG). In contrast, P188 was not as effective as PEG in producing a recovery of CTM functioning. We discuss the likely differences in the mechanisms of action of these two polymers, and the possibilities inherent in a combined treatment.

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Advances in three-dimensional reconstruction of the experimental spinal cord injury

Cited by 16 publications

References 27 publications

The pre-lymphatic pathway, the rooths of the lymphatic system in breast tissue: a 3D study

The pre-lymphatic pathway, the rooths of the lymphatic system in breast tissue: a 3D study

Double labeling serial sections to enhance three-dimensional imaging of injured spinal cord

Subcutaneous tri‐block copolymer produces recovery from spinal cord injury

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