Advances in understanding of the oxysterol-binding protein homologous in yeast and filamentous fungi

Qiu, Shangkun; Zeng, Bin

doi:10.1007/s10123-019-00056-6

Cited by 3 publications

(3 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, OSBP and Osh proteins are being studied extensively worldwide, especially the functions of Osh proteins in S. cerevisiae, Candida albicans, and Aspergillus nestoris . However, studies on Osh proteins in A. oryzae are limited ( Qiu and Zeng, 2019 ). Our study showed that A. oryzae harbors five Osh family proteins, among which Osh1 has been reported previously ( Ma et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Aspergillus oryzae accelerates the conversion of ergosterol to ergosterol peroxide by efficiently utilizing cholesterol

et al. 2022

Self Cite

View full text Add to dashboard Cite

It is well-known that excessive cholesterol leads to hypercholesterolemia, arteriosclerosis, coronary heart disease, stroke, and other diseases, which seriously threatens human health. Lactobacillus, a prokaryote, is reported to utilize cholesterol in the environment. However, little research focuses on the cholesterol utilization by eukaryote. Hence, the objectives of the present study were to investigate the mechanism of cholesterol utilization by the eukaryote and determine the role of oxysterol binding protein in this process. Our results showed for the first time that Aspergillus oryzae, a food-safe filamentous fungus, can utilize cholesterol efficiently. Our results also demonstrated that cholesterol utilization by A. oryzae might promote the conversion of ergosterol to ergosterol peroxide. Osh3, an oxysterol binding protein, can bind sterols (e.g., cholesterol, ergosterol, and ergosterol peroxide) and plays an important role in sterols transportation. This research is of considerable significance for developing low-fat food and cholesterol-lowering probiotics.

show abstract

Section: Discussionmentioning

confidence: 99%

Aspergillus oryzae accelerates the conversion of ergosterol to ergosterol peroxide by efficiently utilizing cholesterol

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ankyrin repeats domain-containing protein (TINF00399) is localized on the cytoplasmic membrane during transient expression in onion epidermal cells (Zhang et al, 2010). The oxysterol binding protein (TINF02166) is an important non-vesicular trafficking protein involved in the transportation of lipids in eukaryotic cells, and may also participate in membrane formation (Qiu and Zeng, 2019). Another four DEPs were involved in the formation of membrane organelles, such as vacuoles and vesicles, as well as transportation processes.…”

Section: Figurementioning

confidence: 99%

Comparative proteomics reveals the mechanism of cyclosporine production and mycelial growth in Tolypocladium inflatum affected by different carbon sources

Wang,

Liu,

Mao

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Cyclosporine A (CsA) is a secondary cyclopeptide metabolite produced by Tolypocladium inflatum that is widely used clinically as an immunosuppressant. CsA production and mycelial growth differed when T. inflatum was cultured in different carbon source media. During early fermentation, CsA was preferred to be produced in fructose medium, while the mycelium preferred to accumulate in sucrose medium. On the sixth day, the difference was most pronounced. In this study, high-throughput comparative proteomics methods were applied to analyze differences in protein expression of mycelial samples on day 6, revealing the proteins and mechanisms that positively regulate CsA production related to carbon metabolism. The differences included small molecule acid metabolism, lipid metabolism, organic catabolism, exocrine secretion, CsA substrate Bmt synthesis, and transcriptional regulation processes. The proteins involved in the regulation of mycelial growth related to carbon metabolism were also revealed and were associated with waste reoxidation processes or coenzyme metabolism, small molecule synthesis or metabolism, the stress response, genetic information or epigenetic changes, cell component assembly, cell wall integrity, membrane metabolism, vesicle transport, intramembrane localization, and the regulation of filamentous growth. This study provides a reliable reference for CsA production from high-efficiency fermentation. This study provides key information for obtaining more CsA high-yielding strains through metabolic engineering strategies.

show abstract

“…ORPs are conserved in fungi (Qiu and Zeng 2019 ), and Y. lipolytica has four ORP genes, OSH1 , OSH3 , OSH4 , and OSH6 (Fig. 3 A).…”

Section: Introductionmentioning

confidence: 99%

Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica

Fukuda

2023

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Yarrowia lipolytica, a dimorphic yeast belonging to the Ascomycota, has potent abilities to utilize hydrophobic compounds, such as n-alkanes and fatty acids, as carbon and energy sources. Yarrowia lipolytica can synthesize and accumulate large amounts of lipids, making it a promising host to produce various lipids and convert n-alkanes to useful compounds. For advanced use of Y. lipolytica in these applications, it is necessary to understand the metabolism of these hydrophobic compounds in this yeast and the underlying molecular mechanisms. In this review, current knowledge on the n-alkane metabolism and how this is regulated in Y. lipolytica is summarized. Furthermore, recent studies revealed that lipid transfer proteins are involved in the utilization of n-alkanes and the regulation of cell morphology in response to n-alkanes. This review discusses the roles of membrane lipids in these processes in Y. lipolytica.

show abstract

Advances in understanding of the oxysterol-binding protein homologous in yeast and filamentous fungi

Cited by 3 publications

References 81 publications

Aspergillus oryzae accelerates the conversion of ergosterol to ergosterol peroxide by efficiently utilizing cholesterol

Aspergillus oryzae accelerates the conversion of ergosterol to ergosterol peroxide by efficiently utilizing cholesterol

Comparative proteomics reveals the mechanism of cyclosporine production and mycelial growth in Tolypocladium inflatum affected by different carbon sources

Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica

Contact Info

Product

Resources

About