Advances of anthocyanin synthesis regulated by plant growth regulators in fruit trees

Wang, Lu; Yang, Shicai; Ni, Junbei; Teng, Yuanwen; Bai, Songling

doi:10.1016/j.scienta.2022.111476

Cited by 22 publications

(13 citation statements)

References 146 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytokinins have been reported to enhance the biosynthesis of anthocyanin. 20 , 21 In our study, we exposed Malus spectabilis leaves to a 20 μM concentration of the cytokinins analog 6-benzylaminopurine (6-BA) for a period of 3 days. Subsequently, we measured the content of anthocyanin in the leaves, and extracted total RNA from the leaves to perform RT-qPCR experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Further investigation revealed that MsMYB62-like could directly bind to the promoters of the anthocyanin structural genes MsDFR and MsANS , resulting in a decrease in their expression levels. Intriguingly, cytokinins, known enhancers of anthocyanin levels, 20 , 21 exhibited a dual effect by enhancing the expression of MsDFR and MsANS , while simultaneously downregulating the expression of MsMYB62-like . These findings propose a potential mechanism wherein cytokinins suppress the expression of MsMYB62-like , disrupting the MsMYB62-like- MsDFR / MsANS module and ultimately leading to an increase in anthocyanin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MsMYB62-like as a negative regulator of anthocyanin biosynthesis in Malus spectabilis

Tan,

Yang,

Xue

et al. 2024

Plant Signaling & Behavior

View full text Add to dashboard Cite

Crabapple is a valuable tree species in gardens due to its captivating array of flower and leaf colors, rendering it a favored choice in landscaping. The economic and ornamental values of Malus crabapple are closely associated with the biosynthesis of anthocyanin, a pigment responsible for its vibrant hues. The intricate regulation of anthocyanin biosynthesis involves the concerted activity of various genes. However, the specific mechanism governing this process in crabapple warrants in-depth exploration. In this study, we explored the inhibitory role of MsMYB62-like in anthocyanin biosynthesis. We identified MsDFR and MsANS as two downstream target genes of MsMYB62-like. These genes encode enzymes integral to the anthocyanin biosynthetic pathway. The findings demonstrate that MsMYB62-like directly binds to the promoters of MsDFR and MsANS , resulting in the downregulation of their expression levels. Additionally, our observations indicate that the plant hormone cytokinins exert a suppressive effect on the expression levels of MsMYB62-like , while concurrently upregulating MsDFR and MsANS . This study reveals that the MsMYB62-like- MsDFR / MsANS module plays an important role in governing anthocyanin levels in Malus crabapple. Notably, the regulatory interplay is modulated by the plant hormone cytokinins.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MsMYB62-like as a negative regulator of anthocyanin biosynthesis in Malus spectabilis

Tan,

Yang,

Xue

et al. 2024

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…It is a commonly used plant auxin analogue in production. In sweet cherry, it was found that NAA treatment can increase the content of acetyl-CoA carboxylase complex (ACC) and ABA-glucose ester (ABA-GE), and ACC overexpression can increase the content of malonyl-CoA, providing sufficient substrate for the synthesis of chalcones and flavonoids [ 59 ]. While ABA-glucose ester is the main storage form of ABA [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…In sweet cherry, it was found that NAA treatment can increase the content of acetyl-CoA carboxylase complex (ACC) and ABA-glucose ester (ABA-GE), and ACC overexpression can increase the content of malonyl-CoA, providing sufficient substrate for the synthesis of chalcones and flavonoids [ 59 ]. While ABA-glucose ester is the main storage form of ABA [ 59 ]. The results of Luo et al showed that auxin treatment could increase the expression of CHS protein and the accumulation of total flavonoids in grape fruits [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy

et al. 2023

View full text Add to dashboard Cite

To explore the accumulation of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy under treatment with different plant growth regulators, 100 mg/L, 200 mg/L and 300 mg/L cycocel, 100 mg/L, 200 mg/L and 300 mg/L mepiquat chloride and 1 mg/L, 2 mg/L and 3 mg/L naphthalene acetic acid were foliage sprayed on Hypericum attenuatum Choisy plants at the early growth stage. We sampled and determined the important flavonoid contents at the flowering stage. The results showed that the three plant growth regulators had different effects on the accumulation of rutin, hyperoside and quercetin in the leaves, stems and flowers of Hypericum attenuatum Choisy at the flowering stage. After spraying 1 mg/L naphthalene acetic acid at the early growth stage, the rutin contents in the leaves, stems and flowers increased by approximately 60.33%, 223.85% and 192.02%, respectively (P < 0.05). Spraying 100 mg/L mepiquat chloride increased the hyperoside contents in the leaves and flowers by approximately 7.77% and 12.87%, respectively (P < 0.05). Spraying 2 mg/L naphthalene acetic acid significantly increased the quercetin contents in the flowers and leaves by approximately 95.62% and 47.85%, respectively (P < 0.05). Therefore, at the early growth stage, spraying 1 mg/L naphthalene acetic acid significantly increased rutin content, spraying 100 mg/L mepiquat chloride significantly increased hyperoside content, and spraying 2 mg/L naphthalene acetic acid significantly increased quercetin content in Hypericum attenuatum Choisy. In conclusion, the accumulation of flavonoids in Hypericum attenuatum Choisy was regulated by plant growth regulators.

show abstract

“…In angiosperms, anthocyanins play an important role in attracting animals to pollinate and disperse seeds (Landi et al, 2015). The anthocyanin biosynthetic pathway and transcriptional regulatory mechanism have been extensively studied in many species (Jaakola, 2013; Wang et al, 2023). Anthocyanins are biosynthesized by phenylalanine metabolism in the cytoplasm and transferred to the vacuole for storage after a series of modifications.…”

Section: Introductionmentioning

confidence: 99%

E3 ubiquitin ligases SINA4 and SINA11 regulate anthocyanin biosynthesis by targeting the IAA29‐ARF5‐1‐ERF3 module in apple

Li,

Liu,

Wang

et al. 2023

Plant Cell & Environment

View full text Add to dashboard Cite

Auxin/indole‐3‐acetic acid (AUX/IAA) and auxin response factor (ARF) proteins are important components of the auxin signalling pathway, but their ubiquitination modification and the mechanism of auxin‐mediated anthocyanin biosynthesis remain elusive. Here, the ARF MdARF5‐1 was identified as a negative regulator of anthocyanin biosynthesis in apple, and it integrates auxin and ethylene signals by inhibiting the expression of the ethylene response factor MdERF3. The auxin repressor MdIAA29 decreased the inhibitory effect of MdARF5‐1 on anthocyanin biosynthesis by attenuating the transcriptional inhibition of MdERF3 by MdARF5‐1. In addition, the E3 ubiquitin ligases MdSINA4 and MdSINA11 played negative and positive regulatory roles in anthocyanin biosynthesis by targeting MdIAA29 and MdARF5‐1 for ubiquitination degradation, respectively. MdSINA4 destabilized MdSINA11 to regulate anthocyanin accumulation in response to auxin signalling. In sum, our data revealed the crosstalk between auxin and ethylene signals mediated by the IAA29‐ARF5‐1‐ERF3 module and provide new insights into the ubiquitination modification of the auxin signalling pathway.

show abstract

Advances of anthocyanin synthesis regulated by plant growth regulators in fruit trees

Cited by 22 publications

References 146 publications

MsMYB62-like as a negative regulator of anthocyanin biosynthesis in Malus spectabilis

MsMYB62-like as a negative regulator of anthocyanin biosynthesis in Malus spectabilis

Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy

E3 ubiquitin ligases SINA4 and SINA11 regulate anthocyanin biosynthesis by targeting the IAA29‐ARF5‐1‐ERF3 module in apple

Contact Info

Product

Resources

About