Advances of optical miniscopes for in vivo imaging of neural activity in freely moving animals

Chen, Kunpeng; Tian, Zhaoshi; Kong, Lingjie

doi:10.3389/fnins.2022.994079

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…In addition, in recent years, multi-photon microscopes have gradually developed into small integrated imaging systems to achieve dynamic imaging of brain function during a conscious state. 32,33 In contrast, the BBB permeability index data obtained by the new method comes from a larger variety of tissues rather than being focused near a single blood vessel, allowing for a more macroscopic and holistic assessment of vascular function damage in various brain regions. The radiological imaging methods do not require surgery, 27 but the metabolic time after a single injection of gadolinium is more than 3 days, which limits its examination of ux and makes it more suitable for long-term monitoring of pathological models of the central nervous system.…”

Section: Discussionmentioning

confidence: 99%

Visualization of blood-brain barrier disruption in septic mice with the new method based on in vivo imaging technology

Zhang,

Ai,

Zhang

et al. 2024

Preprint

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Objectives Dynamic monitoring of the blood-brain barrier (BBB) functional status in septic mice can helps to explore the pathological mechanisms. Therefore, we proposed a new method for monitoring BBB permeability and apply it to the detection of sepsis models. Methods The new method involves the construction of an optical cranial window and in vivo imaging. We performed dynamic monitoring of BBB permeability and cerebral blood flow (CBF) in cecal ligation puncture (CLP) and endotoxemia (LPS) mice. Results The sensitivity and accuracy of this method were higher than those of Evans blue evaluation. The increase of BBB permeability in CLP group mice was relatively mild and correlated with overall survival (OS), and the damage was irreversible. Contrarily, BBB damage in the LPS group was more acute and severe, unrelated to OS, but recoverable. The CBF decreased significantly in both model mouse groups 24 hours after modeling, but only the CBF proportion decrease in the LPS group was significantly correlated with the increased value of BBB permeability. Within 24 hours after both models were established, the decrease in blood flow in the digestive organs occurred earlier than in the brain and kidneys, and the decrease in small intestine blood flow in the LPS group progressed faster. Conclusion We have successfully demonstrated the feasibility of our novel method to detect BBB permeability in mice. Our results revealed a significant difference in the BBB permeability change trend between the CLP and LPS model mice when survival curves were consistent. Notably, the CLP model mice demonstrated a closer resemblance to clinical patients. Our findings suggest that early-stage brain tissue hypoperfusion has a greater impact on BBB function damage in endotoxemia mice, which is related to the faster progression of blood flow redistribution.

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Section: Discussionmentioning

confidence: 99%

Visualization of blood-brain barrier disruption in septic mice with the new method based on in vivo imaging technology

Zhang,

Ai,

Zhang

et al. 2024

Preprint

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“…Au cours de la dernière décennie, plusieurs systèmes optiques ont été développés dans le but d'enregistrer l'activité neuronale d'animaux se déplaçant librement. Ces systèmes peuvent être regroupés en trois catégories : 1) des microscopes monophotoniques (1P) miniaturisés, 2) des microscopes biphotoniques (2P) ou à trois photons (3P) [10] Une fois qu'une image de référence des neurones a été acquise, il est possible de créer un hologramme ciblant simul-tanément plusieurs neurones que l'on souhaite stimuler à partir de la lumière laser. On peut utiliser un hologramme qui génère plusieurs points focaux qui vont être balayés sur les corps cellulaires [6], ou utiliser plusieurs disques d'illumination qui couvrent l'ensemble du corps cellulaire [7].…”

Section: Contrôle « Tout-optique » Des Circuits Neuronaux Chez Des An...unclassified

Un fibroscope biphotonique pour l’imagerie et la stimulation optogénétique des neurones chez des souris se déplaçant librement

Lorca-Cámara,

Blot,

Accanto

et al. 2023

Med Sci (Paris)

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“…However, targeting specific cell types in mice remains technically challenging and time-consuming. Emerging technologies such as the miniscope allow continuous monitoring of fluorescence-based readouts on a cellular level in live mouse and rat brains [10-12]. However, the type of readout from the miniscope has been restricted to neuronal activity from fluorescent calcium sensors and resolution along the z-axis remains a limiting factor in analyzing deeper cortical tissue.…”

Section: Introductionmentioning

confidence: 99%

Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven byPRKNknockout

Goldman,

Kareva,

Sarrafha

et al. 2024

Preprint

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Cellular processes including lysosomal and mitochondrial dysfunction are implicated in the development and progression of many diseases and disorders, including neurodegenerative diseases and cancer. However, there is limited ability to continuously monitor these sub-cellular processes in real-time, and standardized assays to quantify these differences are lacking. Quantitative visualization of mitochondrial and lysosomal networks is crucial to better understand how these organelles are dysregulated during disease pathogenesis. To address this gap, we developed GEM-SCOPe (Genetically Encoded and Modular SubCellular Organelle Probes), an expandable toolbox of genetically encoded fluorescent markers, introduced into cell cultures by lentiviral transduction, which can be used to interrogate changes in proliferation and lysosomal and mitochondrial function in cell models of disease pathogenesis and progression. Genetically encoded fluorescent markers are a powerful tool to consistently visualize subcellular components in live cells without disrupting cellular processes. The fluorophores in GEM-SCOPe are designed to specifically localize to the desired organelle and illuminate information on localization, distribution, organelle turnover, and intracellular oxidative stress. GEM-SCOPe is modular; it can be expanded to include existing or new fluorophores and targeted to other subcellular structures or cell types, increasing the potential for genetically encoded fluorescent markers to probe any cell-model system or subcellular network in live cell cultures. To validate and illustrate the translational functionality of GEM-SCOPe, we applied it to track and quantify sub-cellular pathophysiology changes associated with Parkinson's Disease mediated by loss of function knockout of the PRKN gene. We expressed GEM-SCOPe in a PRKN knockout induced pluripotent stem cell line, differentiated into astrocytes and neurons, enabling us to track and quantify in real-time disease-associated changes in cellular proliferation, lysosomal distribution, mitochondrial transport and turnover, and reactive oxygen species. Collectively, we demonstrate that GEM-SCOPe is a powerful panel of live-cell fluorescent probes that provide critical insight into the subcellular mechanisms underlying Parkinson's disease in human astrocytes and neurons. GEM-SCOPe can be applied to a diverse range of cellular models of disease to glean an understanding of the underlying mechanisms that promote disease onset and progression.

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Advances of optical miniscopes for in vivo imaging of neural activity in freely moving animals

Cited by 9 publications

References 36 publications

Visualization of blood-brain barrier disruption in septic mice with the new method based on in vivo imaging technology

Visualization of blood-brain barrier disruption in septic mice with the new method based on in vivo imaging technology

Un fibroscope biphotonique pour l’imagerie et la stimulation optogénétique des neurones chez des souris se déplaçant librement

Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven byPRKNknockout

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