Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Wang, Dianri; Wang, Yuhao; Tian, Weidong; Pan, Jian

doi:10.1111/cpr.12572

Cited by 49 publications

(43 citation statements)

References 111 publications

(349 reference statements)

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“…The application of combined DSCs with various scaffolds promotes the function of injured neural tissues and reduces the inflammatory responses. The most common scaffolds applied for neural tissue regeneration and repair include chitosan, heparin-poloxamer, silicone tubes, poly-ε-caprolactone/poly-lactide-co-glycolic acid, and electrospun neuro-supportive scaffolds[ 191 ]. Different scaffolds were used to enhance neural differentiation and promote their neuronal characteristics.…”

Section: Neurological Disordersmentioning

confidence: 99%

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migration, and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific purposes. An enormous variety of scaffolds have been used for tissue engineering with DSCs. Of these, the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis, cell-matrix interactions, degradation of extracellular matrix, organized matrix formation, and the mineralization abilities of DSCs in both in vitro and in vivo conditions. DSCs represent a promising cell source for tissue engineering, especially for tooth, bone, and neural tissue restoration. The purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

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Section: Neurological Disordersmentioning

confidence: 99%

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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“…DPSCs are positive for STRO-1, CD146, CD73, and CD90. Similar to other MSCs, DPSCs are capable of multilineage differentiation, including osteogenesis, chondrogenesis, adipogenesis, and myogenic lineages[ 9 - 15 ]. Regarding neural differentiation, DPSCs have a higher positive ratio for neural markers such as Nestin, GFAP, and s100-beta than other kinds of MSCs[ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Neurotrophic effects of dental pulp stem cells in repair of peripheral nerve after crush injury

Wang

Pan

et al. 2020

WJSC

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BACKGROUND Nerve diseases and injuries, which are usually accompanied by motor or sensory dysfunction and disorder, impose a heavy burden upon patients and greatly reduce their quality of life. Dental pulp stem cells (DPSCs), derived from the neural crest, have many characteristics that are similar to those of neural cells, indicating that they can be an ideal source for neural repair. AIM To explore the potential roles and molecular mechanisms of DPSCs in crushed nerve recovery. METHODS DPSCs were isolated, cultured, and identified by multilineage differentiation and flow cytometry. Western blot and immunofluorescent staining were applied to analyze the expression levels of neurotrophic proteins in DPSCs after neural induction. Then, we collected the secretions of DPSCs. We analyzed their effects on RSC96 cell proliferation and migration by CCK8 and transwell assays. Finally, we generated a sciatic nerve crush injury model in vivo and used the sciatic function index, walking track analysis, muscle weight, and hematoxylin & eosin (H&E) staining to further evaluate the nerve repair ability of DPSCs. RESULTS DPSCs highly expressed several specific neural markers, including GFAP, S100, Nestin, P75, and NF200, and were inclined toward neural differentiation. Furthermore, neural-induced DPSCs (N-DPSCs) could express neurotrophic factors, including NGF, BDNF, and GDNF. The secretions of N-DPSCs could enhance the proliferation and migration of Schwann cells. In vivo , both DPSC and N-DPSC implants alleviated gastrocnemius muscle atrophy. However, in terms of anatomy and motor function, as shown by H&E staining, immunofluorescent staining, and walking track analyses, the repair effects of N-DPSCs were more sustained, potent, and effective than those of DPSCs and the controls. CONCLUSION In summary, this study demonstrated that DPSCs are inclined to differentiate into neural cells. N-DPSCs express neurotrophic proteins that could enhance the proliferation and migration of SCs. Furthermore, our results suggested that N-DPSCs could help crushed nerves with functional recovery and anatomical repair in vivo . Thus, DPSCs or N-DPSCs could be a promising therapeutic cell source for peripheral nerve repair and regeneration.

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“…Dental MSCs have been suggested to promote taste bud regeneration and have promising potential applications in postsurgery tongue reconstruction of patients with tongue cancer [ 106 ]. Interestingly, recent work has also suggested that SHEDs exert a protective effect on the secretory function of the salivary gland and exhibit therapeutic potential for the improvement of hyposalivation in Sjogren's syndrome [ 107 ].…”

Section: Dental Msc-based Therapy For Nondental Diseasesmentioning

confidence: 99%

“…Other dental MSCs have also been applied in the treatment of extraoral diseases like neurodegenerative diseases and autoimmune and orthopedic disorders. Dental MSCs have a remarkable potential to treat neural diseases such as spinal cord injury (SCI) and peripheral nerve injury, like sciatic nerve and superior laryngeal nerve (SLN) injury, owing to their ability to differentiate into neural-like cells and regenerate neural tissue [ 107 ]. SCI is a severe traumatic central nervous system disease resulting in the damage of sensory and motor functions.…”

Section: Dental Msc-based Therapy For Nondental Diseasesmentioning

confidence: 99%

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

Gan

Liu

Cui

et al. 2020

Stem Cells International

100

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Human mesenchymal stem cells (hMSCs) are multipotent cells, which exhibit plastic adherence, express specific cell surface marker spectrum, and have multi-lineage differentiation potential. These cells can be obtained from multiple tissues. Dental tissue-derived hMSCs (dental MSCs) possess the ability to give rise to mesodermal lineage (osteocytes, adipocytes, and chondrocytes), ectodermal lineage (neurocytes), and endodermal lineages (hepatocytes). Dental MSCs were first isolated from dental pulp of the extracted third molar and till now they have been purified from various dental tissues, including pulp tissue of permanent teeth and exfoliated deciduous teeth, apical papilla, periodontal ligament, gingiva, dental follicle, tooth germ, and alveolar bone. Dental MSCs are not only easily accessible but are also expandable in vitro with relative genomic stability for a long period of time. Moreover, dental MSCs have exhibited immunomodulatory properties by secreting cytokines. Easy accessibility, multi-lineage differentiation potential, and immunomodulatory effects make dental MSCs distinct from the other hMSCs and an effective tool in stem cell-based therapy. Several preclinical studies and clinical trials have been performed using dental MSCs in the treatment of multiple ailments, ranging from dental diseases to nondental diseases. The present review has summarized dental MSC sources, multi-lineage differentiation capacities, immunomodulatory features, its potential in the treatment of diseases, and its application in both preclinical studies and clinical trials. The regenerative therapeutic strategies in dental medicine have also been discussed.

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Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Cited by 49 publications

References 111 publications

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Neurotrophic effects of dental pulp stem cells in repair of peripheral nerve after crush injury

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

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