Advances on photodynamic therapy of melanoma through novel ring-fused 5,15-diphenylchlorins

Pereira, Neide; Laranjo, Mafalda; Pina, João; Oliveira, Andreia S. R.; Dias-Ferreira, João; Sánchez-Sánchez, Carles; Casalta-Lopes, João; Gonçalves, Ana Cristina; Sarmento‐Ribeiro, Ana Bela; Piñeiro, Marta; Melo, J. Sérgio Seixas de; Botelho, Maria Filomena; Melo, Teresa M. V. D. Pinho e

doi:10.1016/j.ejmech.2017.12.093

Cited by 22 publications

(40 citation statements)

References 48 publications

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“…The metabolic activity was determined by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; MTT, Sigma Aldrich ® , USA) [22,23,24]. Briefly, cell cultures were washed with PBS and incubated with 0.5 mg·mL −1 MTT, pH = 7.4, at 37 °C for 4 h. Formazan crystals were solubilized in 40 mM hydrochloric acid in isopropanol.…”

Section: Methodsmentioning

confidence: 99%

Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonate-Related Osteonecrosis of the Jaw

et al. 2019

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(1) Background: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is one of the most often seen side effects in patients treated with nitrogen-containing bisphosphonates (BPs), a post-surgical non-healing wound condition. Since calcium phosphate (CP) compounds are able to adsorb zoledronate (ZOL) when used as a drug delivery vehicle, we aimed to verify if these ceramics might have a potential protective effect for soft tissues surrounding surgical osseous wounds. (2) Methods: The chemical reaction between ZOL and CP compounds was evaluated through ultraviolet-visible spectroscopy and elemental analysis. A primary culture of human gingival fibroblasts (HGF) was established as a model to evaluate the cytotoxicity of the association of ZOL (5–500 μM) and of ZOL/biphasic calcium phosphates (BCP). Metabolic activity, cell viability, types of cell death, the cell cycle through, and the migration ability of human gingival fibroblasts were evaluated. (3) Results: ZOL was adsorbed by biphasic calcium phosphate compounds in an aqueous solution. The HGF were sensitive to ZOL toxicity; nevertheless, ZOL/BCP showed a significant protective effect regarding metabolic activity, cell viability, and cell migration. (4) Conclusions: BCP interaction with ZOL reduces or abolishes its toxicity in HGF. This finding represents a potential solution for BRONJ in the case of patients undergoing therapy with ZOL.

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Section: Methodsmentioning

confidence: 99%

Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonate-Related Osteonecrosis of the Jaw

et al. 2019

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“…The reduction of these diester chlorins ( 1 and 2 ) with lithium aluminum hydride led to the corresponding dihydroxymethyl chlorins ( 3 and 4 ). 37,40 Chlorins 1 – 4 present photophysical properties that are adequate to their use as photosensitizers with high absorption at the therapeutic window and moderate to high singlet oxygen quantum yields.…”

Section: Results and Discussionmentioning

confidence: 99%

“…These chlorins were even more photocytotoxic against A375 skin malignant melanoma cells than the corresponding tetraphenyl analogues, presenting IC 50 values as low as 2.9 nM. 40 Furthermore, for one of the derivatives (chlorin 3 ), the cell death outcome, apoptosis versus necrosis, was determined by its concentration. This can be explored to control the type of cell death in order to improve the effectiveness of PDT considering that an inflammatory response resulting from necrotic cell death after PDT can activate the antitumor immune response with implications also on vascular damage.…”

Section: Introductionmentioning

confidence: 99%

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Ring-Fused Diphenylchlorins as Potent Photosensitizers for Photodynamic Therapy Applications: In Vitro Tumor Cell Biology and in Vivo Chick Embryo Chorioallantoic Membrane Studies

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Laranjo²,

Pereira

et al. 2019

ACS Omega

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Ring-fused diphenylchlorins as potent low-dose photosensitizers for photodynamic therapy of bladder carcinoma and esophageal adenocarcinoma are described. All studied molecules were very active against HT1376 urinary bladder carcinoma and OE19 esophageal adenocarcinoma cell lines, showing IC50 values below 50 nM. The in vivo evaluation of the more promising photosensitizer, using an OE19 tumor/chick embryo chorioallantoic membrane model, showed a tumor weight regression of 33% with a single photodynamic therapy treatment with the photosensitizer dose as low as 37 ng/embryo.

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“…Porphyrins can be used in fluorescence imaging, magnetic resonance imaging (MRI), photodynamic therapy (PDT), sonodynamic therapy (SDT), boron neutron capture therapy (BNCT), and radiation therapy. In fact, porphyrins have been widely explored for theranostics [14,15,16,17,18,19,20,21,22,23,24,25].…”

Section: Introductionmentioning

confidence: 99%

A Review on (Hydro)Porphyrin-Loaded Polymer Micelles: Interesting and Valuable Platforms for Enhanced Cancer Nanotheranostics

et al. 2019

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Porphyrins are known therapeutic agents for photodynamic therapy of cancer and also imaging agents for NIR fluorescence imaging, MRI, or PET. A combination of interesting features makes tetrapyrrolic macrocycles suitable for use as theranostic agents whose full potential can be achieved using nanocarriers. This review provides an overview on nanotheranostic agents based on polymeric micelles and porphyrins developed so far.

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Advances on photodynamic therapy of melanoma through novel ring-fused 5,15-diphenylchlorins

Cited by 22 publications

References 48 publications

Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonate-Related Osteonecrosis of the Jaw

Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonate-Related Osteonecrosis of the Jaw

Ring-Fused Diphenylchlorins as Potent Photosensitizers for Photodynamic Therapy Applications: In Vitro Tumor Cell Biology and in Vivo Chick Embryo Chorioallantoic Membrane Studies

A Review on (Hydro)Porphyrin-Loaded Polymer Micelles: Interesting and Valuable Platforms for Enhanced Cancer Nanotheranostics

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