Advancing Array Tomography to Study the Fine Ultrastructure of Identified Neurons in Zebrafish (Danio rerio)

Strobel, Marlene; Helmprobst, Frederik; Pauli, Martin; Heckmann, Manfred; Lillesaar, Christina; Stigloher, Christian

doi:10.1007/978-1-0716-0691-9_4

Cited by 1 publication

(2 citation statements)

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“…Speaking of which, 39 years later Strobel and colleagues presented a modern CLEM protocol to study identified neurons and used the Mauthner neuron as their example (Strobel et al, 2020). They labeled the Mauthner neuron specifically by retrograde tracing with fluorescent rhodamine dextran.…”

Section: Brain and Spinal Cordmentioning

confidence: 99%

“…They used TEM to confirm the presence of myelin sheaths in their purification fractions and they used immunogold labeling on cryosections to localize proteins. Speaking of which, 39 years later Strobel and colleagues presented a modern CLEM protocol to study identified neurons and used the Mauthner neuron as their example (Strobel et al, 2020). They labeled the Mauthner neuron specifically by retrograde tracing with fluorescent rhodamine dextran.…”

Section: Myelinmentioning

confidence: 99%

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Studying zebrafish nervous system structure and function in health and disease with electron microscopy

Markert

2023

Dev Growth Differ

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Zebrafish (Danio rerio) is a well‐established model for studying the nervous system. Findings in zebrafish often inform studies on human diseases of the nervous system and provide crucial insight into disease mechanisms. The functions of the nervous system often rely on communication between neurons. Signal transduction is achieved via release of signaling molecules in the form of neuropeptides or neurotransmitters at synapses. Snapshots of membrane dynamics of these processes are imaged by electron microscopy. Electron microscopy can reveal ultrastructure and thus synaptic processes. This is crucial both for mapping synaptic connections and for investigating synaptic functions. In addition, via volumetric electron microscopy, the overall architecture of the nervous system becomes accessible, where structure can inform function. Electron microscopy is thus of particular value for studying the nervous system. However, today a plethora of electron microscopy techniques and protocols exist. Which technique is most suitable highly depends on the research question and scope as well as on the type of tissue that is examined. This review gives an overview of the electron microcopy techniques used on the zebrafish nervous system. It aims to give researchers a guide on which techniques are suitable for their specific questions and capabilities as well as an overview of the capabilities of electron microscopy in neurobiological research in the zebrafish model.This article is protected by copyright. All rights reserved.

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Section: Brain and Spinal Cordmentioning

confidence: 99%

Section: Myelinmentioning

confidence: 99%