Advancing biofilm management through nanoformulation strategies: a review of dosage forms and administration routes

Simsekli, Oyku; Bilinmis, Irfan; Celik, Sumeyye; Arık, Gizem; Baba, Abdullah Yucel; Karakucuk, Alptug

doi:10.1080/1061186x.2023.2270619

Cited by 4 publications

(2 citation statements)

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“…However, this frequently required concentrations higher than the FICI value. This might be associated with the greater resilience of bacteria within biofilms and the penetration capacity of the drugs …”

Section: Resultsmentioning

confidence: 99%

“…This might be associated with the greater resilience of bacteria within biofilms and the penetration capacity of the drugs. 13 Scanning Electron Microscopy Shows Inhibition of Biofilm Formation. To visually assess the impact of azomycin−colistin on the bacterial population, morphology, and structure of the ECC biofilm, scanning electron microscopy (SEM) was employed.…”

Section: Checkerboard Assay Shows Synergy Of Azomycin−mentioning

confidence: 99%

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Azomycin Orchestrate Colistin-Resistant Enterobacter cloacae Complex’s Colistin Resistance Reversal In Vitro and In Vivo

Hu,

Chen,

Zhao

et al. 2024

ACS Infect. Dis.

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The Enterobacter cloacae complex (ECC) is a group of nosocomial pathogens that pose a challenge in clinical treatment due to its intrinsic resistance and the ability to rapidly acquire resistance. Colistin was reconsidered as a last-resort antibiotic for combating multidrug-resistant ECC. However, the persistent emergence of colistin-resistant (COL-R) pathogens impedes its clinical efficacy, and novel treatment options are urgently needed. We propose that azomycin, in combination with colistin, restores the susceptibility of COL-R ECC to colistin in vivo and in vitro. Results from the checkerboard susceptibility, time-killing, and live/dead bacterial cell viability tests showed strong synergistic antibacterial activity in vitro. Animal infection models suggested that azomycin−colistin enhanced the survival rate of infected Galleria mellonella and reduced the bacterial load in the thighs of infected mice, highlighting its superior in vivo synergistic antibacterial activity. Crystal violet staining and scanning electron microscopy unveiled the in vitro synergistic antibiofilm effects of azomycin−colistin. The safety of azomycin and azomycin−colistin at experimental concentrations was confirmed through cytotoxicity tests and an erythrocyte hemolysis test. Azomycin−colistin stimulated the production of reactive oxygen species in COL-R ECC and inhibited the PhoPQ two-component system to combat bacterial growth. Thus, azomycin is feasible as a colistin adjuvant against COL-R ECC infection.

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Section: Resultsmentioning

confidence: 99%

Section: Checkerboard Assay Shows Synergy Of Azomycin−mentioning

confidence: 99%