2024
DOI: 10.1101/2024.05.08.593163
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Advancing chloroplast synthetic biology through high-throughput plastome engineering ofChlamydomonas reinhardtii

René Inckemann,
Tanguy Chotel,
Cedric K. Brinkmann
et al.

Abstract: Chloroplast synthetic biology holds promise for developing improved crops through improving the function of plastids. However, chloroplast engineering efforts face limitations due to the scarcity of genetic tools and the low throughput of plant-based systems. To address these challenges, we here establishedChlamydomonas reinhardtiias a prototyping chassis for chloroplast synthetic biology. We developed an automation workflow that enables the generation, handling, and analysis of thousands of transplastomic str… Show more

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