Advancing mechanobiology by performing whole-cell patch clamp recording on mechanosensitive cells subjected simultaneously to dynamic stretch events

Coulon, Etienne de; Dellenbach, Christian; Rohr, Stephan

doi:10.1016/j.isci.2021.102041

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Recently, de Coulon et al. (2021) reported large stretch‐activated inward currents in NIH 3T3 cells, a cell line derived from fibroblasts. Thus, it is possible that the responses to deformation that we observed might have been caused by myofibroblasts or other cell types.…”

Section: Discussionmentioning

confidence: 99%

“…Further studies with dedicated systems, such as the platform developed by de Coulon et al. (2021), might provide an appropriate answer in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Given that the time course of caveolae incorporation and possible capacitance changes is unknown, we can, nevertheless, speculate that an increase in capacitance could have contributed to the slow return of beat rate towards baseline after a few seconds. Further studies with dedicated systems, such as the platform developed by de Coulon et al (2021), might provide an appropriate answer in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Although we minimized the number of myofibroblasts by preplating the dissociated cells and limited their proliferation with bromodeoxyuridine, we cannot exclude the presence of a residual number of such cells, which would influence the automaticity in our cultures in control conditions and especially upon stretch. Recently, de Coulon et al (2021) reported large stretch-activated inward currents in NIH 3T3 cells, a cell line derived from fibroblasts. Thus, it is possible that the responses to deformation that we observed might have been caused by myofibroblasts or other cell types.…”

Section: Is It Possible That Myofibroblasts Caused the Observed Effects?mentioning

confidence: 99%

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Active force generation contributes to the complexity of spontaneous activity and to the response to stretch of murine cardiomyocyte cultures

Nayir

Lacour²,

Kučera

2022

The Journal of Physiology

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Cardiomyocyte cultures exhibit spontaneous electrical and contractile activity, as in a natural cardiac pacemaker. In such preparations, beat rate variability exhibits features similar to those of heart rate variability in vivo. Mechanical deformations and forces feed back on the electrical properties of cardiomyocytes, but it is not fully elucidated how this mechano‐electrical interplay affects beating variability in such preparations. Using stretchable microelectrode arrays, we assessed the effects of the myosin inhibitor blebbistatin and the non‐selective stretch‐activated channel blocker streptomycin on beating variability and on the response of neonatal or fetal murine ventricular cell cultures against deformation. Spontaneous electrical activity was recorded without stretch and upon predefined deformation protocols (5% uniaxial and 2% equibiaxial strain, applied repeatedly for 1 min every 3 min). Without stretch, spontaneous activity originated from the edge of the preparations, and its site of origin switched frequently in a complex manner across the cultures. Blebbistatin did not change mean beat rate, but it decreased the spatial complexity of spontaneous activity. In contrast, streptomycin did not exert any manifest effects. During the deformation protocols, beat rate increased transiently upon stretch but, paradoxically, also upon release. Blebbistatin attenuated the response to stretch, whereas this response was not affected by streptomycin. Therefore, our data support the notion that in a spontaneously firing network of cardiomyocytes, active force generation, rather than stretch‐activated channels, is involved mechanistically in the complexity of the spatiotemporal patterns of spontaneous activity and in the stretch‐induced acceleration of beating. Key points Monolayer cultures of cardiac cells exhibit spontaneous electrical and contractile activity, as in a natural cardiac pacemaker. Beating variability in these preparations recapitulates the power‐law behaviour of heart rate variability in vivo. However, the effects of mechano‐electrical feedback on beating variability are not yet fully understood. Using stretchable microelectrode arrays, we examined the effects of the contraction uncoupler blebbistatin and the non‐specific stretch‐activated channel blocker streptomycin on beating variability and on stretch‐induced changes of beat rate. Without stretch, blebbistatin decreased the spatial complexity of beating variability, whereas streptomycin had no effects. Both stretch and release increased beat rate transiently; blebbistatin attenuated the increase of beat rate upon stretch, whereas streptomycin had no effects. Active force generation contributes to the complexity of spatiotemporal patterns of beating variability and to the increase of beat rate upon mechanical deformation. Our study contributes to the understanding of how mechano‐electrical feedback influences heart rate variability.

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Section: Discussionmentioning

confidence: 99%

“…Further studies with dedicated systems, such as the platform developed by de Coulon et al. (2021), might provide an appropriate answer in the future.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Is It Possible That Myofibroblasts Caused the Observed Effects?mentioning

confidence: 99%

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Active force generation contributes to the complexity of spontaneous activity and to the response to stretch of murine cardiomyocyte cultures

Nayir

Lacour²,

Kučera

2022

The Journal of Physiology

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Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs

Roeterink,

Casadevall i Solvas,

Collins

et al. 2024

Adv Healthcare Materials

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Mechanotransduction is the process whereby cells convert mechanical signals into electrochemical responses, where mechanosensitive proteins mediate this interaction. To characterize these critical proteins, numerous techniques have been developed that apply forces and measure the subsequent cellular responses. While these approaches have given insight into specific aspects of many such proteins, subsequent validation and cross‐comparison between techniques remain difficult given significant variations in reported activation thresholds and responses for the same protein across different studies. Accurately determining mechanosensitivity responses for various proteins, however, is essential for understanding mechanotransduction and potential physiological implications, including therapeutics. This critical review provides an assessment of current and emerging approaches used for mechanosensitive ion channel and G‐Coupled Receptors (GPCRs) stimulation and measurement, with a specific focus on the ability to quantitatively measure mechanosensitive responses.

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Ion channel mediated mechanotransduction in immune cells

Atcha

Jairaman

Evans

et al. 2021

Current Opinion in Solid State and Materials Science

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Advancing mechanobiology by performing whole-cell patch clamp recording on mechanosensitive cells subjected simultaneously to dynamic stretch events

Cited by 5 publications

References 34 publications

Active force generation contributes to the complexity of spontaneous activity and to the response to stretch of murine cardiomyocyte cultures

Active force generation contributes to the complexity of spontaneous activity and to the response to stretch of murine cardiomyocyte cultures

Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs

Ion channel mediated mechanotransduction in immune cells

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