Advantages of a miniature pig model in research on human hereditary hearing loss

Guo, Weiwei; Yang, Shiming

doi:10.1016/j.joto.2015.11.001

Cited by 10 publications

(9 citation statements)

References 10 publications

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“…Interestingly, the same microstructure, porous tubes with parallel alignment, is inherent for skeletal muscular tissue of pig Sus scrofa domesticus (see cryo-SEM images on Figs 6 and S11 ). Pigs are known to be a species closest to humans: the vital organs in pigs and humans share high level of similarity in structure 36 . That is why they have been largely used in medical research 37 .…”

Section: Resultsmentioning

confidence: 99%

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Ivashchenko

Przysiecka

Peplińska

et al. 2018

Sci Rep

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Combination therapy remains one of the most promising and intensively developed direction in cancer treatment. This study is aimed to combine and investigate the anticancer properties of silver nanoparticles (NPs) and Amanita muscaria mushroom in gel formulation. For this, hyaluronic acid was used as gel-forming agent, whereas Amanita muscaria extract was used as capping agent during silver and ultrasmall iron oxide (MAg) NPs synthesis. Amanita muscaria compounds formed NP’s surface layer and contributed anticancer properties, whereas silver NPs contributed anticancer, fluorescence and photoactive properties to the gel. Physicochemical characterization included X-ray diffraction (XRD), microscopies (SEM, cryo-SEM, TEM, confocal fluorescence), spectrofluorometric method, thermogravimetric analysis (TGA), dynamic light scattering (DLS) techniques, energy dispersive (EDS), Fourier transform infrared (FTIR) and ultraviolet–visible (UV-Vis) spectroscopies, zeta-potential and rheological measurements. Microstructure analysis of hyaluronic acid/MAg NPs gel was performed by cryo-SEM technique. We showed that hyaluronic acid is a perfect gel-forming agent from both biomedical and technological points of view. It is well-mixed with MAg NPs forming stable gel formulation; high homogeneity of hyaluronic acid/MAg NPs gel was shown by SEM EDS elemental mapping. Microstructure of the gel was found to be highly ordered and consisted of domains from perforated parallel tubular structures. This finding expanded our understanding of gels and broke the stereotype of gel structure as chaotic network of fibers. Cytotoxicity studies performed on 2D and 3D HeLa cell cultures pointed to a high potential of hyaluronic acid/MAg NPs gel for local treatment of cancer. Cell response was found to be significantly different for 2D and 3D cell cultures that was related to their different cytoarhitecture and gene expression. Thus, the results of the cellular spheroids viability showed that they were significantly more resistant to the cytotoxic action of MAg NPs and their gel formulation than 2D cell culture. Hyaluronic acid used as gelling agent in gel formulation was found to increase an effectiveness of active components (MAg NPs, Amanita muscaria extract) probably improving their transport inside HeLa spheroids.

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Section: Resultsmentioning

confidence: 99%

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Ivashchenko

Przysiecka

Peplińska

et al. 2018

Sci Rep

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“…In medical laboratory animals, the pig is the closest species to human in evolutionary terms, with the exception of primates. As an animal model, the pig is highly regarded by many scientific fields, including comparative biology, developmental biology and medical genetics (Guo and Shi-ming 2015).…”

Section: Discussionmentioning

confidence: 99%

An Observational Study of Pigs Exposed to Radiation

Piao

Iitsuka

et al. 2019

Agricultural Implications of the Fukushima Nuclear Accident (III)

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On June 28, 2011, 26 pigs were rescued from the alert area, 17 km northwest of the Fukushima Daiichi Nuclear Power Plant, where radiation levels were approximately 1.9-3.8 μSv/h. The pigs were transferred outside of the radiation alert area to the Animal Resource Science Center (ARSC), The University of Tokyo. It was confirmed by the farm owner that the pigs were never fed radiationcontaminated concentrate and they had access to uncontaminated groundwater (http://www.env.go.jp/press/press.php?serial=16323) while living inside the radiation alert area; however, radiocesium was detected in the rescued pigs' organs, testis/ovary, spleen, liver, kidney, psoas major, urine, and blood, within nine months after the nuclear disaster. Radiocesium levels in samples collected in early January 2012 were significantly lower than those collected in either early or late September 2011, indicating a continuing decrease in radiation levels over that duration. Radiocesium was not detected in organs collected in August 2012. In September 2011, the authors of the present study visited a local farm to collect samples from pigs who remained inside the radiation alert area. Radiocesium concentration in these pigs was nearly ten times higher than from the rescued pigs. 36 Seven of the 16 sows rescued were able to reproduce. The present study showed that the age of sow significantly affected their ability to reproduce. These 7 sows had 15 parturition events and birthed 166 piglets, including two malformed piglets. However, the present study confirmed that body weight did not affect reproductive performance. The average body weight of reproductive and non-reproductive sows was 226.3 versus 230.6 kg, respectively.Hematology analysis showed that red blood cells (RBC) were lower in rescued pigs than in the non-exposed pigs. The level of HGB, HCT, MCV, and MCH, which are all related to RBC counts, were consistent with the changes in RBC between the two groups. The plasma biochemical indexes that relate to liver and kidney functions also showed differences between the two groups of pigs.The present study was not scientifically designed and did not contain proper control groups for all tests. As a result, we are not able to conclude the exact effects of the radiation exposure to the pigs' health.

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“…Owing to their physiological and anatomical similarity to humans, pigs can serve as medical models for multiple human diseases. For example, there are reports on pig models for xenotransplantation (Blusch et al, 2002; Mariscal et al, 2018), wound healing (Sullivan et al, 2001), dental and orofacial research (Wang et al, 2007), gastrohelcoma (Tian et al, 2009), hearing loss (Guo & Yang, 2015) and neurodegenerative disorders (Holm et al, 2016). Research efforts in recent years have made tremendous strides toward the genome‐wide editing of pigs, including the inactivation of porcine endogenous retroviruses (Yang et al, 2015) and germline engineering (Yue et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The de novo assembly of a European wild boar genome revealed unique patterns of chromosomal structural variations and segmental duplications

Chen

Zhong

et al. 2022

Animal Genetics

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The rapid progress of sequencing technology has greatly facilitated the de novo genome assembly of pig breeds. However, the assembly of the wild boar genome is still lacking, hampering our understanding of chromosomal and genomic evolution during domestication from wild boars into domestic pigs. Here, we sequenced and de novo assembled a European wild boar genome (ASM2165605v1) using the long‐range information provided by 10× Linked‐Reads sequencing. We achieved a high‐quality assembly with contig N50 of 26.09 Mb. Additionally, 1.64% of the contigs (222) with lengths from 107.65 kb to 75.36 Mb covered 90.3% of the total genome size of ASM2165605v1 (~2.5 Gb). Mapping analysis revealed that the contigs can fill 24.73% (93/376) of the gaps present in the orthologous regions of the updated pig reference genome (Sscrofa11.1). We further improved the contigs into chromosome level with a reference‐assistant scaffolding method. Using the ‘assembly‐to‐assembly’ approach, we identified intra‐chromosomal large structural variations (SVs, length >1 kb) between ASM2165605v1 and Sscrofa11.1 assemblies. Interestingly, we found that the number of SV events on the X chromosome deviated significantly from the linear models fitting autosomes (R2 > 0.64, p < 0.001). Specifically, deletions and insertions were deficient on the X chromosome by 66.14 and 58.41% respectively, whereas duplications and inversions were excessive on the X chromosome by 71.96 and 107.61% respectively. We further used the large segmental duplications (SDs, >1 kb) events as a proxy to understand the large‐scale inter‐chromosomal evolution, by resolving parental‐derived relationships for SD pairs. We revealed a significant excess of SD movements from the X chromosome to autosomes (p < 0.001), consistent with the expectation of meiotic sex chromosome inactivation. Enrichment analyses indicated that the genes within derived SD copies on autosomes were significantly related to biological processes involving nervous system, lipid biosynthesis and sperm motility (p < 0.01). Together, our analyses of the de novo assembly of ASM2165605v1 provides insight into the SVs between European wild boar and domestic pig, in addition to the ongoing process of meiotic sex chromosome inactivation in driving inter‐chromosomal interaction between the sex chromosome and autosomes.

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Advantages of a miniature pig model in research on human hereditary hearing loss

Cited by 10 publications

References 10 publications

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

Gel with silver and ultrasmall iron oxide nanoparticles produced with Amanita muscaria extract: physicochemical characterization, microstructure analysis and anticancer properties

An Observational Study of Pigs Exposed to Radiation

The de novo assembly of a European wild boar genome revealed unique patterns of chromosomal structural variations and segmental duplications

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