Advantages of aggregation-induced luminescence microspheres compared with fluorescent microspheres in immunochromatography assay with sandwich format

Wei, Xiaxia; Chen, Xirui; Liu, Zilong; Xu, Ge; Chen, Guoxin; Lin, Xiangkai; Zhan, Wenchao; Huang, Xiaolin; Xiong, Yonghua

doi:10.1016/j.aca.2023.340869

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…This approach held great potential to optimize detection performance of immunochromatography platforms, as depicted in Fig. 13 B to D [ 113 , 114 ]. Additionally, highly luminescent quantum dot beads (QBs) were prepared as an alternative to AuNPs.…”

Section: Immune Colloidal Gold Technique For Pct Detectionmentioning

confidence: 99%

Progress in Procalcitonin Detection Based on Immunoassay

Huang,

Zu,

Zhang

et al. 2024

Research

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Procalcitonin (PCT) serves as a crucial biomarker utilized in diverse clinical contexts, including sepsis diagnosis and emergency departments. Its applications extend to identifying pathogens, assessing infection severity, guiding drug administration, and implementing theranostic strategies. However, current clinical deployed methods cannot meet the needs for accurate or real-time quantitative monitoring of PCT. This review aims to introduce these emerging PCT immunoassay technologies, focusing on analyzing their advantages in improving detection performances, such as easy operation and high precision. The fundamental principles and characteristics of state-of-the-art methods are first introduced, including chemiluminescence, immunofluorescence, latex-enhanced turbidity, enzyme-linked immunosorbent, colloidal gold immunochromatography, and radioimmunoassay. Then, improved methods using new materials and new technologies are briefly described, for instance, the combination with responsive nanomaterials, Raman spectroscopy, and digital microfluidics. Finally, the detection performance parameters of these methods and the clinical importance of PCT detection are also discussed.

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Section: Immune Colloidal Gold Technique For Pct Detectionmentioning

confidence: 99%

Progress in Procalcitonin Detection Based on Immunoassay

Huang,

Zu,

Zhang

et al. 2024

Research

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“…However, for traditional fluorescent dyes, due to the aggregation-caused quenching (ACQ) effect, especially at high concentrations, the sensitivity and stability of fluorescent dyes can be affected seriously, causing a low signal-to-noise ratio and photobleaching [ 58 ]. To overcome these problems, Wei et al [ 59 ] utilized aggregation-induced emission luminogen (AIEgen)-embedded fluorescent microspheres (AIEFMs) instead of fluorescein dyes for the detection of procalcitonin (PCT), as shown in Figure 4 E. For the synthesis of AIEFM@mAbs, the AIEFM reacted with EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) firstly, and then the carboxyl groups of AIEFMS coupled with the amino groups of the detected antibody (anti-PCT mAbs) to serve as the signal reporter (see Figure 4 E i). The nitrocellulose (NC) membrane was modified by two kinds of antibodies, anti-PCT pAbs (capture antibody at T line) and goat anti-mouse IgG (anti-antibody at C line).…”

Section: Strategies For Nanoparticle-based Aptasensorsmentioning

confidence: 99%

Section: Strategies For Nanoparticle-based Aptasensorsmentioning

confidence: 99%

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Ouyang

Chen

et al. 2023

Biosensors

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The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has gained extensive attention for biomolecular detection due to its high selectivity, affinity, reproducibility, and robustness. Concurrently, biosensing with nanoparticles has been widely used for its high carrier capacity, stability and feasibility of incorporating optical and catalytic activity, and enhanced diffusivity. Biosensors based on aptamers and nanoparticles utilize the combination of their advantages and have become a promising technology for detecting of a wide variety of biomolecules with high sensitivity, reliability, specificity, and detection speed. Via various sensing mechanisms, target biomolecules have been quantified in terms of optical (e.g., colorimetric and fluorometric), magnetic, and electrical signals. In this review, we summarize the recent advances in and compare different aptamer–nanoparticle-based biosensors by nanoparticle types and detection mechanisms. We also share our views on the highlights and challenges of the different nanoparticle-aptamer-based biosensors.

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“…They emit fluorescence with high brightness by restricting intramolecular rotation, vibration, and motion in the aggregated state. [14] Due to the AIE effect, the luminescence signal increases the concentration of luminescent labels, which may increase the sensitivity of immunoassay. Therefore, this section will focus on the great potential of using AIE to enhance the sensitivity of current immunoassays, such as LFIA and fluorescence/Enzyme-linked immunosorbent assays (FLISA/ELISA).…”

Section: Aggregation Induced Emission Labelsmentioning

confidence: 99%

“…Nanoprobes are detection tools that use nanomaterials or nanostructures as carriers and bind to target substances through specific recognition molecules. [5] Commonly used nanoprobes include colloidal gold (CG), [6,7] quantum dots (QDs), [8,9] magnetic nanoparticles (MNPs), [10,11] nanoenzymes, [12] aggregationinduced emission (AIE) particles, [13,14] up-conversion nanoparticles (UCNPs). [15] Nanoprobes possess small size, extensive specific surface area, excellent biocompatibility, potent modifiability, and can combine with numerous signal conversion and amplification techniques.…”

Section: Introductionmentioning

confidence: 99%

Advances in nanoprobes‐based immunoassays

Zhang,

Huang,

Jin

et al. 2023

BMEMat

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Immunoassay is a powerful technique that uses highly specific antigen‐antibody interactions to detect biochemical targets such as proteins and toxins. As a diagnostic tool, immunoassay is employed in the screening, diagnosis, and prognosis of diseases, which are crucial for the grasp and control of patient conditions in clinical practice. With the rapid development of nanotechnology, immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity, specificity, stability, and versatility. These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays. In this paper, we review the recent advances in various types of nanoprobes for immunoassays, such as colloidal gold, quantum dots, magnetic nanoparticles, nanozymes, aggregation‐induced emission, and up‐conversion nanoparticles. The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth. We also compare their detection ranges and limits in different immunoassay methods, such as lateral flow immunoassays, fluorescent immunoassays, and surface‐enhanced Raman scattering immunoassays. Moreover, we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development. This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

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Advantages of aggregation-induced luminescence microspheres compared with fluorescent microspheres in immunochromatography assay with sandwich format

Cited by 13 publications

References 40 publications

Progress in Procalcitonin Detection Based on Immunoassay

Progress in Procalcitonin Detection Based on Immunoassay

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Advances in nanoprobes‐based immunoassays

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