Advantages of fluorescent microspheres compared with colloidal gold as a label in immunochromatographic lateral flow assays

Xie, Quan-Yuan; Wu, Yanhua; Xiong, Qirong; Xu, Hengyi; Xiong, Yonghua; Liu, Kun; Jin, Yong; Lai, Weihua

doi:10.1016/j.bios.2013.11.002

Cited by 170 publications

(82 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Edwards and Baeumner (2006) and Khreich et al (2008) used liposomes to encapsulate fluorescent dyes, getting higher sensitivity when using larger particles, with limits of detection close to 20 pg/mL. Commercial fluorescent microspheres which can be used on LFSs also are available (Xie et al, 2014;Wang et al, 2014b;Zhou et al, 2014). Interestingly, Xie et al (2014) evaluated the optimal quantity of antibodies required to be conjugated with their microspheres and compared it with AuNPs, resulting that when using microspheres this quantity can be reduced four times; moreover, even using less quantity of antibodies in the labels, the sensitivity, the working range and the limit of detection are improved in comparison to AuNPs assays.…”

Section: Other Fluorescent Materialsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

View full text Add to dashboard Cite

Lateral flow biosensors (LFBs) are paper-based devices which permit the performance of low-cost and fast diagnostics with good robustness, specificity, sensitivity and low limits of detection. The use of nanoparticles (NPs) as labels play an important role in the design and fabrication of a lateral flow strip (LFS). The choice of NPs and the corresponding detection method directly affect the performance of these devices. This review discusses aspects related to the application of different nanomaterials (e.g. gold nanoparticles, carbon nanotubes, quantum dots, up-converting phosphor technologies, and latex beads, between others) in LFBs. Moreover, different detection methods (colorimetric, fluorescent, electrochemical, magnetic, etc.) and signal enhancement strategies (affording secondary reactions or modifying the architecture of the LFS) as well as the use of devices such as smartphones to mediate the response of LFSs will be analyzed.

show abstract

Section: Other Fluorescent Materialsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

View full text Add to dashboard Cite

show abstract

“…The most widely used format for such assays uses gold nanoparticles for colorimetric detection (Meng et al, 2014;Nakayama et al, 2014). The antibodies bind tightly to the surfaces of the gold nanoparticles when correctly coupled, providing long-term stability in liquid and solid forms (Xie et al, 2014). Many studies have reported the successful detection of pathogens using this assay (Pengsuk et al, 2013;Sun et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A novel dynamic flow immunochromatographic test (DFICT) using gold nanoparticles for the serological detection of Toxoplasma gondii infection in dogs and cats

Jiang

Liu

Chen

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

A novel dynamic flow immunochromatographic test (DFICT) is proposed for rapid assay utilizing Toxoplasma gondii as a model. The test is based on a proprietary technology that combines the principles of immunochromatography and fluid dynamics. Gold nanoparticles conjugated to staphylococcal protein A (SPA) were prepared in liquid form and used as signal vehicles. T. gondii-specific recombinant antigens and SPA were sprayed onto a nitrocellulose membrane in strips at positions designated as T and C, respectively. The DFICT is performed by applying a 100 µL aliquot of liquid gold-SPA conjugate to the reagent hole and a 5 μL aliquot of serum sample to the sample hole. The results were observable within 5 min by the naked eye. The lowest detectable limit of the assay was determined as the highest dilution (1:320) of positive serum. No cross-reaction of the antibodies with other related canine or feline pathogens was observed. The DFICT can be stored for 12 months at 4°C or 6 months with no loss of sensitivity or specificity. A high degree of consistency was observed between the DFICT and the standard ELISA kit, supporting the reliability of the novel test strip. The introduction of a liquid gold nanoparticle conjugate reagent provides this method with several attractive characteristics, such as ease of manufacture, low sample volume requirements, high selectivity and high efficiency. This method opens a novel pathway for rapid diagnostic screening and field analysis.

show abstract

“…With the development of biotechnology and biosensor technology [3,4] and biochip technology [5][6][7], POCT becomes more convenient and fast, which makes it more suitable for family medical care and community hospitals. Fluorescence immunochromatographic chip has the advantages of high sensitivity, strong specificity and fast detection speed [8,9], which is widely used in POCT, such as detection of alpha fetoprotein [10] and chloramphenicol [11] and so on.…”

Section: Introductionmentioning

confidence: 99%

Smartphone-Based Fluorescent Diagnostic System for Immunochromatographic Chip

Hou¹,

Wang²,

Yang³

et al. 2017

Nano BioMed ENG

View full text Add to dashboard Cite

In order to achieve fast and quantitative detection of fluorescence immunochromatographic chip, a rapid detection system based on smartphone has been developed. In this system, fluorescent signal from quantum dots (QDs) on lateral flow test strips (LFTSs) can be accurately extracted, and the system also can calculate the concentration of the analyte. The method of extraction and recognition of fluorescence signal intensity can be applied to different fluorescent chip detection systems. Based on the fluorescence tomography chip image, a specific program is used for image acquisition, processing and data handling. The Sobel operator algorithm was used in the software, which improved greatly the ability of distinguishing between the test area and the background boundary information. Extracting the components from the red format of the fluorescent strips, the high-signal intensity and sensitivity were achieved. The simulation results show that the proposed method can be applied to the detection system of fluorescence immunochromatographic chip. The experimental results show that the signal intensity has a good correlation with the concentration of immunoassay, which indicates the detection system can extract the intensity of fluorescence signal of the chip.

show abstract

Advantages of fluorescent microspheres compared with colloidal gold as a label in immunochromatographic lateral flow assays

Cited by 170 publications

References 17 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

A novel dynamic flow immunochromatographic test (DFICT) using gold nanoparticles for the serological detection of Toxoplasma gondii infection in dogs and cats

Smartphone-Based Fluorescent Diagnostic System for Immunochromatographic Chip

Contact Info

Product

Resources

About