Advantages of full spectrum flow cytometry

Sanders, Claire K.; Mourant, Judith R.

doi:10.1117/1.jbo.18.3.037004

Cited by 26 publications

(19 citation statements)

References 10 publications

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“…Various methods for spectral unmixing have already been suggested, for example alternating least squares or generalized linear models with Poisson noise . Sony provides unmixing software with its instrument .…”

Section: Discussionmentioning

confidence: 99%

Multispectral flow cytometry: The consequences of increased light collection

Feher¹,

Volkmann²,

Kirsch³

et al. 2016

Cytometry Pt A

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In recent years, multispectral flow cytometry systems have come to attention. They differ from conventional flow cytometers in two key ways: a multispectral flow cytometer collects the full spectral information at the single cell level and the detector configuration is fixed and not explicitly tuned to a particular staining panel. This brings about clear hardware advantages, as a closed system should be highly stable, and ease-of-use should be improved if used in conjunction with custom unmixing software. An open question remains: what are the benefits of multispectral over conventional flow cytometry in terms of sensitivity and resolution? To probe this, we use Q (detection efficiency) and B (background) values and develop a novel "multivariate population overlap factor" to characterize the cytometer performance. To verify the usefulness of our factor, we perform representative experiments and compare our overlap factor to Q and B. Finally, we conclude that the increased light collection of multispectral flow cytometry does indeed lead to increased sensitivity, an improved detection limit, and a higher resolution.

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Section: Discussionmentioning

confidence: 99%

Multispectral flow cytometry: The consequences of increased light collection

Feher¹,

Volkmann²,

Kirsch³

et al. 2016

Cytometry Pt A

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“…In view of this, the determination of antitesticular antibodies could be a promising tool, as the characteristic antibodies are likely to be produced during chronic inflammation [142]. Recently, the advancement of new technology, such as full-spectrum cytometry and single-cell sequencing, allows us to analyze samples more comprehensively and precisely [143,144]. The multidimensional application of these techniques might give us unique characteristic information for chronic orchitis, like fingerprints, enabling precise and personal therapy strategies.…”

Section: Therapeutic Options For Chronic Orchitismentioning

confidence: 99%

T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes

Gong

Zeng

et al. 2020

IJMS

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The immune privilege of the testes is necessary to prevent immune attacks to gamete-specific antigens and paternal major histocompatibility complex (MHC) antigens, allowing for normal spermatogenesis. However, infection and inflammation of the male genital tract can break the immune tolerance and represent a significant cause of male infertility. Different T cell subsets have been identified in mammalian testes, which may be involved in the maintenance of immune tolerance and pathogenic immune responses in testicular infection and inflammation. We reviewed the evidence in the published literature on different T subtypes (regulatory T cells, helper T cells, cytotoxic T cells, γδ T cells, and natural killer T cells) in human and animal testes that support their regulatory roles in infertility and the orchitis pathology. While many in vitro studies have indicated the regulation potential of functional T cell subsets and their possible interaction with Sertoli cells, Leydig cells, and spermatogenesis, both under physiological and pathological processes, there have been no in situ studies to date. Nevertheless, the normal distribution and function of T cell subsets are essential for the immune privilege of the testes and intact spermatogenesis, and T cell-mediated immune response drives testicular inflammation. The distinct function of different T cell subsets in testicular homeostasis and the orchitis pathology suggests a considerable potential of targeting specific T cell subsets for therapies targeting chronic orchitis and immune infertility.

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“…The recent commercialization of spectral flow cytometry (first reported 20 years ago by groups in Los Alamos, New Mexico, USA) provides a path toward more sophisticated analysis of fluorescence-based signals 12,13 . Unlike conventional flow cytometry, spectral analyzers collect the complete fluorescence emission spectrum from all fluorochromes at once.…”

Section: Expanding Multiplexed Depth For Flow Cytometrymentioning

confidence: 99%

Single-cell technologies for monitoring immune systems

et al. 2014

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The complex heterogeneity of cells, and their interconnectedness with each other, are major challenges to identifying clinically relevant measurements that reflect the state and capability of the immune system. Highly multiplexed, single-cell technologies may be critical for identifying correlates of disease or immunological interventions as well as for elucidating the underlying mechanisms of immunity. Here we review limitations of bulk measurements and explore advances in single-cell technologies that overcome these problems by expanding the depth and breadth of functional and phenotypic analysis in space and time. The geometric increases in complexity of data make formidable hurdles for exploring, analyzing and presenting results. We summarize recent approaches to making such computations tractable and discuss challenges for integrating heterogeneous data obtained using these single-cell technologies.

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Advantages of full spectrum flow cytometry

Cited by 26 publications

References 10 publications

Multispectral flow cytometry: The consequences of increased light collection

Multispectral flow cytometry: The consequences of increased light collection

T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes

Single-cell technologies for monitoring immune systems

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