Advantages of high speed scanning for microprobe analysis of biological samples

Llabador, Y.; Bertault, D.; Gouillaud, J. C.; Moretto, Ph.

doi:10.1016/0168-583x(90)90289-7

Cited by 54 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the Centre d'Etudes Nucléaires de Bigazzi et al, 1993 and. Bordeaux-Gradignan (CENBG) the samples were analyzed on the 'Microbeam Line' of a 4 MV Van de Graaff accelerator (Llabador et al, 1990). In the vacuum sample chamber of this nuclear microprobe up to 15 samples could be introduced together, fixed on a rotating stage allowing us to make measurements on several points of their surface.…”

Section: Pixementioning

confidence: 99%

The use of SEM-EDS, PIXE and EDXRF for obsidian provenance studies in the Near East: a case study from Neolithic Çatalhöyük (central Anatolia)

Poupeau

Bourdonnec

Carter

et al. 2010

Journal of Archaeological Science

View full text Add to dashboard Cite

Section: Pixementioning

confidence: 99%

The use of SEM-EDS, PIXE and EDXRF for obsidian provenance studies in the Near East: a case study from Neolithic Çatalhöyük (central Anatolia)

Poupeau

Bourdonnec

Carter

et al. 2010

Journal of Archaeological Science

View full text Add to dashboard Cite

“…This encouraging result is even more significant because gelatin standards, commonly used for testing and quantitative analysis of biological materials (Marshall, 1982), are much more sensitive to beam interactions than animal or plant tissues (Marshall, 1980). Mass losses during electron and proton bombardment at room temperature are evident and well documented, especially when high currents are applied (Cholewa et al, 1991;Llabador et al, 1990;Themner, 1991;Themner et al, 1990;Van Lierde, 1995;Zierold, 1988). It is therefore beneficial to perform analyses of fragile materials at low temperature.…”

Section: Limits Of Quantitative Analysismentioning

confidence: 74%

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

Tylko¹,

Mesjasz‐Przybyłowicz²,

Przybyłowicz³

2006

Microscopy Res & Technique

View full text Add to dashboard Cite

Elemental microanalysis of biological material in the frozen-hydrated state using in-vacuum proton induced X-ray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear microprobe (NMP). The analyzed material was frozen in propane cooled by liquid nitrogen, fractured, carbon coated, and transferred onto the cold stage (100 K) of the nuclear microprobe chamber. Micro-PIXE and simultaneous proton backscattering was performed using a 3 MeV proton beam. Quantitative results were obtained by the standardless method, and tested using 20% gelatin standards. Monitoring of the gas composition inside the system by means of mass spectrometry performed before, during, and after proton bombardment showed good stability of the analyzed material for proton currents not exceeding 150 pA. Average concentrations of light elements (C, N, O, and indirectly H) were also obtained by the proton backscattering technique. No losses of elements measurable by particle-induced X-ray emission (PIXE) during proton irradiation were found during repetitive, short analyses of the same micro areas of gelatin standards. Measurements of thick sections of selected plant and animal material in the frozen-hydrated state-leaf sections of the plant Senecio anomalochrous Hilliard (Asteraceae) and larvae of Chysolina pardalina Fabricius (Chrysomelidae)-showed very good preservation of morphology and elemental distribution. Limits of detection of the order of a few micro g g(-1) were obtained for most elements.

show abstract

“…A total of 25 geological samples and 541 artefacts have been analysed thus far at the C2RMF in the AGLAE facility (Accélérateur Grand Louvre d'Analyses Elémentaires; Calligaro et al, 2002), which external micro-beam set-up allows for the analysis of larger samples (Le Bourdonnec et al, 2005. At the CENBG, the AIFIRA platform (Applications Interdisciplinaires de Faisceaux d'Ions en Région Aquitaine) presents a nuclear microprobe capable to analyse very small samples, due to its 5-μm beam diameter (Llabador et al, 1990). A total of 30 obsidian geological samples and 134 artefacts were thus analysed at this facility.…”

Section: Iba-pixementioning

confidence: 99%

On sourcing obsidian assemblages from the Mediterranean area: analytical strategies for their exhaustive geochemical characterisation

Orange

Bourdonnec

Bellot‐Gurlet

et al. 2017

Journal of Archaeological Science: Reports

View full text Add to dashboard Cite

This paper presents an overview of the work conducted by our research group in the Mediterranean area. Initiated in the 1990s by the late Gérard Poupeau, our research relies on international and multidisciplinary collaborations to endeavour archaeological and anthropological issues linked to the diffusion and consumption of the obsidian raw material during the Neolithic period. Our line of action is to develop flexibly unique analytical strategies, tailored to each obsidian assemblage considered for a sourcing study. Drawing its strength from the complementarity of the methods available within our group, i.e. visual characterisation, SEM-EDS, ED-XRF, pXRF, PIXE, and LA-ICP-MS, this approach allows for the exhaustive and non-destructive analysis of those assemblages, thus optimising the potential of sourcing studies. Working hand in hand with archaeologists, the results are closely integrated to the information brought by the typological and technological characteristics of the artefacts, in the aim to reconstruct an overview of the obsidian economy at site level, but also to replace it in a broader — regional and supra-regional — context

show abstract

Advantages of high speed scanning for microprobe analysis of biological samples

Cited by 54 publications

References 9 publications

The use of SEM-EDS, PIXE and EDXRF for obsidian provenance studies in the Near East: a case study from Neolithic Çatalhöyük (central Anatolia)

The use of SEM-EDS, PIXE and EDXRF for obsidian provenance studies in the Near East: a case study from Neolithic Çatalhöyük (central Anatolia)

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

On sourcing obsidian assemblages from the Mediterranean area: analytical strategies for their exhaustive geochemical characterisation

Contact Info

Product

Resources

About