Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

The present paper deals with the electrochemical behavior of three types of sensors based on modified screen-printed electrodes (SPEs): a sensor based on carbon nanofibers (CNF/SPE), a sensor based on nanofibers of carbon modified with gold nanoparticles (CNF-GNP/SPE) and a biosensor based on nanofibers of carbon modified with gold nanoparticles and tyrosinase (CNF-GNP-Ty/SPE). To prepare the biosensor, the tyrosinase (Ty) was immobilized on the surface of the electrode already modified with carbon nanofibers and gold nanoparticles, by the drop-and-dry technique. The electrochemical properties of the three electrodes were studied by cyclic voltammetry in electroactive solutions, and the position and shape of the active redox peaks are according to the nature of the materials modifying the electrodes. In the case of ferulic acid, a series of characteristic peaks were observed, the processes being more intense for the biosensor, with the higher sensitivity and selectivity being due to the immobilization of tyrosinase, a specific enzyme for phenolic compounds. The calibration curve was subsequently created using CNF-GNP-Ty/SPE in ferulic acid solutions of various concentrations in the range 0.1–129.6 μM. This new biosensor allowed low values of the detection threshold and quantification limit, 2.89 × 10−9 mol·L−1 and 9.64 × 10−9 mol·L−1, respectively, which shows that the electroanalytical method is feasible for quantifying ferulic acid in real samples. The ferulic acid was quantitatively determined in three cosmetic products by means of the CNF-GNP-Ty/SPE biosensor. The results obtained were validated by means of the spectrometric method in the infrared range, the differences between the values of the ferulic acid concentrations obtained by the two methods being under 5%.

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“…The values of Γ are similar to those obtained for other biosensors based on tyrosinase used in detecting phenolic compounds [ 68 , 69 ].…”

Section: Resultssupporting

confidence: 81%

Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers–Gold Nanoparticles–Tyrosinase for the Detection of Ferulic Acid in Cosmetics

Bounegru

Apetrei

2020

Sensors

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“…The obtained value for K ap M was (7.4 � 0.6) � 10 −6 mol L −1 , this value is within expected ranges for biomimetic sensors using electron mediators (Nassef, Radi, and O'Sullivan 2006;Apetrei et al 2012;Wong et al 2015).…”

Section: Resultssupporting

confidence: 80%

Determination of Metribuzin with a Cobalt Phthalocyanine-Modified Carbon Paste Electrode

et al. 2018

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Metribuzin is a widely used pesticide with a half-life of up to 2 months and high affinity to organic matter that should be carefully monitored. Here is reported the development of a modified carbon paste electrode using cobalt phthalocyanine as a biomimetic catalyst for the determination of metribuzin. The diffusion coefficient of metribuzin at room temperature was (59 � 16) � 10 −6 cm 2 s −1 and the apparent Michaelis-Menten constant (K ap M) was (7.4 � 0.6) � 10 −6 mol L −1. Electroanalytical measurements were performed by amperometry and the following figures of merit were obtained: limit of detection of 5.3 � 10 −6 mol L −1 (1.14 mg L −1), linear range up to 2.0 � 10 −4 mol L −1 , recoveries above 93% (performed on spiked water samples), interday repeatability of 4.5% and intraday repeatability of 3.5%, and suitable selectivity.

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“…This encouraging improvement verified that, apart from the hydrophobic characteristic of the PBAP coating that can induce interfacial activation of lipase, its biocompatible property also helped to maintain the lipase activity. The advantages of using biocompatible and biomimetic supports for enzyme immobilization have also been reported by others [30][31][32]. The biocompatible property also helped to maintain the lipase activity.…”

Section: Lipase Immobilization and Activity Retentionmentioning

confidence: 54%

Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface

et al. 2016

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Abstract:In this work, poly(n-butylamino)(allylamino)phosphazene (PBAP) was synthesized and tethered on polypropylene microporous membrane (PPMM) with the aim of offering a biocompatible and, at the same time, moderately hydrophobic microenvironment to lipase for the first time. Lipase from Candida rugosa was used and the influence of membrane surface conditions on the activities of immobilized lipases was evaluated. Water contact angle measurement as well as field emission scanning electron microscopy were used to characterize the morphology of the modified membranes. The results showed an improvement in the adsorption capacity (26.0 mg/m 2 ) and activity retention (68.2%) of the immobilized lipases on the PBAP-modified PPMM. Moreover, the lipases immobilized on the modified PPMM showed better thermal and pH stability.

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Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

Cited by 15 publications

References 41 publications

Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers–Gold Nanoparticles–Tyrosinase for the Detection of Ferulic Acid in Cosmetics

Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers–Gold Nanoparticles–Tyrosinase for the Detection of Ferulic Acid in Cosmetics

Determination of Metribuzin with a Cobalt Phthalocyanine-Modified Carbon Paste Electrode

Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface

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