Adventitial versus intimal liposome-mediated ex vivo transfection of canine saphenous vein grafts with endothelial nitric oxide synthase gene

Kalra, Manju; Jost, Corey J.; Severson, Sandra R.; Miller, Virginia M.

doi:10.1067/mva.2000.109211

Cited by 26 publications

(16 citation statements)

References 39 publications

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“…Further evidence for a beneficial role of adventitial NO delivery comes from gene transfer studies where it has been shown that adventitia-dependent arterial relaxation occurs following transduction with recombinant eNOS gene [25] and that adventitial transfection of eNOS gene is more effective in reducing graft occlusion than intravascular delivery [26]. …”

Section: Discussionmentioning

confidence: 99%

Does Periadventitial Fat-Derived Nitric Oxide Play a Role in Improved Saphenous Vein Graft Patency in Patients Undergoing Coronary Artery Bypass Surgery?

Dashwood¹,

Dooley²,

et al. 2007

J Vasc Res

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Background/Aims: The saphenous vein is commonly used for coronary artery bypass surgery but its patency is poor. Vascular damage occurs during conventional surgery. However, patency improves when the graft is harvested with minimal surgical trauma, partly due to preservation of vascular endothelial nitric oxide synthase (eNOS) and tissue sources of nitric oxide (NO), a factor possessing both dilatory and anti-proliferative properties. Apart from these grafts exhibiting an intact luminal endothelium they are harvested complete with a surrounding cushion of tissue, much of which is fat. Methods: Immunostaining for eNOS was performed on vein graft sections and reverse-transcriptase polymerase chain reaction and Western blotting were used to identify eNOS mRNA and protein. NO synthase activity was measured using the citrulline assay. Results: Immunohistochemistry identified eNOS staining of vein graft segments, including dense staining of the cushion of perivascular fat and associated structures surrounding the vein. eNOS protein was confirmed in both the vein and surrounding fat by Western blot analysis. Using the citrulline assay, the perivascular fat and underlying vein possessed comparable NO synthase activity. Conclusions: Our observations suggest that perivascular fat-derived NO plays a beneficial role in saphenous veins harvested atraumatically and used as grafts in patients undergoing coronary artery bypass surgery.

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Section: Discussionmentioning

confidence: 99%

Does Periadventitial Fat-Derived Nitric Oxide Play a Role in Improved Saphenous Vein Graft Patency in Patients Undergoing Coronary Artery Bypass Surgery?

Dashwood¹,

Dooley²,

et al. 2007

J Vasc Res

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show abstract

“…Reverse transcription reaction was performed with 5 g of pure total RNA using SuperScript™ II reverse transcriptase (Invitrogen). Synthesized cDNA was amplified by PCR using the following primers: (1) vWF, 15 sense 5Ј-TGG ATG AGC TTT TGC AGA CC-3Ј, antisense 5Ј-GTG GGA GCC GTC GTG GTA CT-3Ј; (2) endothelial nitric oxide synthase (eNOS), 16 sense 5Ј-TCA ACC AGT ACT ACA GCT CC-3Ј, antisense 5Ј-GTG GTT GCA GAT GTA GGT GA-3Ј; (3) kinase-insert domain-containing receptor (KDR), 17 sense 5Ј-TTC CTG ACC TTG GAG CAT CT-3Ј, antisense 5Ј-AGT CCA GCA TGG TCT GGT AC-3Ј; (4) ribosome s17, 15 sense 5Ј-GAA GGC GGC CCG GGT CAT CA-3Ј, antisense 5Ј-GTA GGC TGA GTG ACC TG-3Ј. PCR was carried out for 30 cycles of denaturing (94°C, 30 s), annealing (60°C, 30 s), and extension (72°C, 45 s) with a final extension at 72°C for 7 min.…”

Section: Semiquantitative Analysis Using Rt-pcrmentioning

confidence: 99%

Enhancement of in vivo endothelialization of tissue‐engineered vascular grafts by granulocyte colony‐stimulating factor

Cho

Lim

Chu

et al. 2005

J Biomedical Materials Res

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Successful reconstruction of large-diameter blood vessel in humans has been demonstrated using the tissue engineering technique, but improvement in patency of small-diameter bioartificial vascular graft remains a great challenge. This study reports that granulocyte colony-stimulating factor (G-CSF) can enhance in vivo endothelialization of tissue-engineered vascular grafts, which could be used to improve patency of small-diameter vascular graft. Vascular grafts were tissue engineered with decellularized canine abdominal aortas and canine autologous bone marrow-derived cells. Prior to cell seeding onto decellularized graft matrices, bone marrow-derived cells were induced to differentiate into endothelial cells and smooth muscle cells. The cell-seeded vascular grafts were implanted into the abdominal aortas of bone marrow donor dogs. Before and after graft implantation, G-CSF was administered subcutaneously to the dogs (n = 3). The grafts implanted into the dogs not receiving G-CSF were used as controls (n = 3). Eight weeks after implantation, grafts in both groups showed regeneration of vascular tissues including endothelium and smooth muscle. Importantly, endothelium formation was more extensive in the G-CSF-treated grafts than in the control grafts, as assessed with reverse transcription polymerase chain reaction, western blot, and immunohistochemistry. In addition, intimal hyperplasia was significantly reduced in the G-CSF-treated grafts compared to the control grafts. This study suggests that G-CSF administration could be applied to improve patency of small-diameter tissue-engineered vascular grafts.

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“…All of these vectors, however, exhibit fairly low penetrance into vascular tissue when the basement membrane is intact. [6][7][8][9][10][11][12] Therefore, most strategies have stipulated preemptive mechanical denudation of the endothelium, followed by delivery of very high doses of vector. Even with these modifications, the observed duration of reporter gene expression has been limited, and most of its biological activity is undetectable by the end of the first week.…”

Section: Introductionmentioning

confidence: 99%

Prevention of restenosis by a herpes simplex virus mutant capable of controlled long-term expression in vascular tissue in vivo

et al. 2001

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Neointimal hyperplasia resulting from vascular smooth muscle cell (SMC) proliferation and luminal migration is the major cause of autologous vein graft failure following vascular coronary or peripheral bypass surgery. Strategies to attenuate SMC proliferation by the delivery of oligonucleotides or genes controlling cell division rely on the use of high concentrations of vectors, and require pre-emptive disruption of the endothelial cell layer. We report a genetically engineered herpes simplex virus (HSV-1) mutant that, in an in vivo rabbit

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Adventitial versus intimal liposome-mediated ex vivo transfection of canine saphenous vein grafts with endothelial nitric oxide synthase gene

Cited by 26 publications

References 39 publications

Does Periadventitial Fat-Derived Nitric Oxide Play a Role in Improved Saphenous Vein Graft Patency in Patients Undergoing Coronary Artery Bypass Surgery?

Does Periadventitial Fat-Derived Nitric Oxide Play a Role in Improved Saphenous Vein Graft Patency in Patients Undergoing Coronary Artery Bypass Surgery?

Enhancement of in vivo endothelialization of tissue‐engineered vascular grafts by granulocyte colony‐stimulating factor

Prevention of restenosis by a herpes simplex virus mutant capable of controlled long-term expression in vascular tissue in vivo

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