Adventitious regeneration in styrian oil pumpkin in relation to the endoreduplication pattern and induced tetraploidy on fusaric acid-supplemented media

Košmrlj, Kristina; Kladnik, Aleš; Bohanec, B.

doi:10.1007/s10725-014-9961-5

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…The basal region had higher frequency (79.17-83.33%) than the apical region (5.23-8.25%) after four weeks culture on regeneration medium. Similar results were reported by Monacelli et al (1988), Tabei et al (1993) and Košmrlj et al (2015). These phenomena suggested that the proximal end of each cotyledon segment regenerated adventitious shoots more effectively than the distal end and the basal region showed polarity.…”

Section: Discussionsupporting

confidence: 89%

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

Zhao¹,

XiaoWei²,

Fan³

2015

Afr. J. Biotechnol.

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A highly efficient regeneration system is a prerequisite step for successful genetic transformation of watermelon cultivars (Citrullus lanatus L.). The objective of this study was to establish efficient in vitro plant regeneration for three watermelon cultivars. To achieve optimal conditions for adventitious shoot induction, the 5-day-old explants (cotyledon base portion, apical portion and hypocotyl) of three cultivars were placed on MB 5 media supplemented with different concentrations and combinations of growth regulators (1.0 to 10.0 mg L-1 6-benzyladenine (BA) and 0 to 1.0 mg L-1 indole acetic acid (IAA)); the explants from seedling of different development stages (0 to 10 d) were cultured on MB 5 medium containing 2.0 mg L-1 BA and 0.2 mg L-1 IAA for investigating the effect of age on adventitious shoots initiation; besides, 5-day-old seedlings were grown on optimal regeneration medium supplemented with different concentrations of kanamycin for screening the lowest lethal concentration for adventitious shoots. The results show that the basal region of cotyledon showed higher frequency of shoot formation (79.17-83.33%) than the apical region (5.23-8.25%); high percentage of shoots regeneration was induced from 5-day-old cotyledons base portion cultured on MB 5 containing 1 or 2 mg L-1 BA; the 100 mg L-1 kanamycin proved to be the optimal concentration for screening the transformants. Our results provide an efficient stable regeneration system for genetic transformation of watermelon.

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Section: Discussionsupporting

confidence: 89%

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

Zhao¹,

XiaoWei²,

Fan³

2015

Afr. J. Biotechnol.

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Meta-topolin: Advantages and Disadvantages for In Vitro Propagation

Ambros

Novikova

2021

Meta-Topolin: A Growth Regulator for Plant Biotechnology and Agriculture

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Effect of culture filtrates of Fusarium fungi on oat callus cultures

Lugovtsova,

Stupko,

Neshumaeva

2023

Sib. vestn. s-h. nauki

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The effect of three concentrations (30, 40 and 50%) of the culture filtrate (CF) of the genus Fusarium fungi (F. sporotrichioides, F. poae, F. equiseti, F. oxysporum) on the growth and development parameters of oat callus culture was evaluated. CF at the callus proliferation stage was used to select somaclonal cell lines with mycotoxin tolerance traits. Prior to this, callusogenesis induction was carried out on the Murashige-Skoog (MS) medium with 3 mg/l 2,4-D and 2 mg/l UIC in the culture of immature embryos of the Tyumensky Golozerny, Tubinsky, Zolotoy Pochatok and Talisman varieties. The material for the experiment was selected from the ears of the plants grown in the summer period of 2016–2018 on the experimental fields in the Krasnoyarsk forest-steppe. Callus size was recorded when transplanted onto the proliferation media (MS + 1.5 mg/l 2,4-D), control and CF-containing media. After 30 days of cultivation, callus growth, signs of necrosis and organogenesis were noted. At the end of the experiment, the number of the regenerants formed was counted. The presence of CF in the proliferation medium already at a concentration of 40% provided a decrease in the proliferative activity and increased the frequency of necrosis by at least 50%. Similar results were obtained at the CF level of 50%. On the media with F. poae CF, the reduction of callus viability reached 60–70%. The calluses that remained viable under these conditions had a frequency of regenerant formation and organogenesis 2–3 times higher than the samples that were not influenced by the selective factor. This is particularly pronounced when F. sporotrichioides CF is added. However, this effect was not observed when F. oxysporum CF was applied. This may be due to the differences in the composition of the mycotoxin complex of this mushroom species from the others used in the study. For further work on the technology of creating oat forms with resistance to mycotoxins of the Fusarium genus fungi, it is assumed to use a level of selective pressure not lower than 40%.

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Adventitious regeneration in styrian oil pumpkin in relation to the endoreduplication pattern and induced tetraploidy on fusaric acid-supplemented media

Cited by 3 publications

References 35 publications

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

Meta-topolin: Advantages and Disadvantages for In Vitro Propagation

Effect of culture filtrates of Fusarium fungi on oat callus cultures

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