Adventitious rhizogenesis in Basilicum polystachyon (L.) Moench callus and HPLC analysis of phenolic acids

Das, Sunanda; Sultana, Kaniz Wahida; Chandra, Indrani

doi:10.1007/s11738-021-03317-y

Cited by 5 publications

(6 citation statements)

References 33 publications

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“…The negative effect of phenolic compounds in root formation is associated with stimulating IAA oxidation [58]. The rosmarinic and p-comaric acids were determined in callus and adventitious roots of Basilicum polystachyon (L.) Moench [59]. Additionally, the higher ability to root formation in young individuals and the immature phase to mature phase transition were proved in research on forests woody plants [60].…”

Section: Discussionmentioning

confidence: 99%

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Polyphenolic Acid Changes in Stem Cuttings of Rosa Cultivars in Relation to Phenological Stage and Rooting Enhancers

Monder

Pacholczak

2023

Agronomy

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Biostimulants and rooting enhancers, i.e., auxins, affect many aspects of plant development. The experiment in this paper focused on the response of single-node rose semi-woody cuttings to rhizogenesis-enhancing preparations based on plant extracts in terms of changes in polyphenolic acid content. The shoots were cut at four stages of flowering development: (i) flower buds closed, (ii) open flower, (iii) immediately after petal shedding, (iv) 7–14 days after petal shedding. The experimental material consisted of six old, once-flowering rose cultivars (‘Duchesse d’Angoulême’, ‘Hurdals’, ‘Maiden’s Blush’, ‘Mousseuse Rouge’, Rosa beggeriana ‘Polstjårnan’, R. helenae ‘Semiplena’). The following rooting-enhancers were applied: commercial powder containing (i) 0.4% indolebutyric acid (IBA) or (ii) 0.2% naphthalene acetic acid (NAA) or commercial plant-extract mixtures named in the experiment, i.e., (iii) Seaweed Preparation, (iv) Humic Preparation, and (v) Plant Preparation, and (vi) the control cuttings, which remained untreated. The level of polyphenolic acids was determined before and after rooting. The content of polyphenolic acids had a tendency to decrease during the period of rhizogenesis for all cultivars and all phenological stages. Changes in polyphenolics were affected by all the rooting enhancers, but the contents of these compounds before and after rooting was not found to unambiguously correlate with either the final rooting percentage or quality of cuttings.

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Section: Discussionmentioning

confidence: 99%

“…However, the monophenols and m-diphenols, due to stimulation IAA oxidation, decrease adventitious root formation ability in plants [58]. The promotion or inhibition of root formation in the case of phenolic compounds depends on their chemical type and physiological and biochemical role [58,59].…”

Section: Discussionmentioning

confidence: 99%

Polyphenolic Acid Changes in Stem Cuttings of Rosa Cultivars in Relation to Phenological Stage and Rooting Enhancers

Monder

Pacholczak

2023

Agronomy

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“…Surface sterilization was performed using 0.1% w / v mercuric chloride for 45 s in a Biosafety Cabinet A2 (Biobase Inc., Jinan, China) under aseptic conditions. The explant was thoroughly rinsed three times with Milli-Q water after sterilization [ 34 , 50 ].…”

Section: Methodsmentioning

confidence: 99%

“…Standard solutions of each acid were prepared by dissolving 1 mg in 1 mL of methanol and were used to quantify the phenolic compounds present in the leaves of B. polystachyon . The quantitative determination of phenolic compounds was carried out following the proposed method [ 50 , 60 ].

…”

Section: Methodsmentioning

confidence: 99%

Unveiling the Dual Nature of Heavy Metals: Stressors and Promoters of Phenolic Compound Biosynthesis in Basilicum polystachyon (L.) Moench In Vitro

Das,

Sultana,

Mondal

et al. 2023

Plants

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The global industrial revolution has led to a substantial rise in heavy metal levels in the environment, posing a serious threat to nature. Plants synthesize phenolic compounds under stressful conditions, which serve as protective agents against oxidative stress. Basilicum polystachyon (L.) Moench is an herbaceous plant of the Lamiaceae family. Some species within this family are recognized for their capacity to remediate sites contaminated with heavy metals. In this study, the effects of mercury (II) chloride and lead (II) nitrate on the in vitro propagation of B. polystachyon were investigated. Shoot tips from in vitro plantlets were cultured in Murashige and Skoog’s (MS) media with heavy metals ranging from 1 to 200 µM to induce abiotic stress and enhance the accumulation of phenolic compounds. After three weeks, MS medium with 1 µM of lead (II) supported the highest shoot multiplication, and the maximum number of roots per explant was found in 100 µM of lead (II), whereas a higher concentration of heavy metals inhibited shoot multiplication and root development. The plantlets were hardened in a greenhouse with a 96% field survival rate. Flame atomic absorption spectroscopy (FAAS) was used to detect heavy metal contents in plant biomass. At both 200 µM and 50 µM concentrations, the greatest accumulation of mercury (II) was observed in the roots (16.94 ± 0.44 µg/g) and shoots (17.71 ± 0.66 µg/g), respectively. Similarly, lead (II) showed the highest accumulation in roots (17.10 ± 0.54 µg/g) and shoots (7.78 ± 0.26 µg/g) at 200 µM and 50 µM exposures, respectively. Reverse-phase high-performance liquid chromatography (RP-HPLC) identified and quantified various phenolic compounds in B. polystachyon leaves, including gallic acid, caffeic acid, vanillic acid, p-coumaric acid, ellagic acid, rosmarinic acid, and trans-cinnamic acid. These compounds were found in different forms, such as free, esterified, and glycosylated. Mercury (II)-exposed plants exhibited elevated levels of vanillic acid (1959.1 ± 3.66 µg/g DW), ellagic acid (213.55 ± 2.11 µg/g DW), and rosmarinic acid (187.72 ± 1.22 µg/g DW). Conversely, lead (II)-exposed plants accumulated higher levels of caffeic acid (42.53±0.61 µg/g DW) and p-coumaric acid (8.04 ± 0.31 µg/g DW). Trans-cinnamic acid was the predominant phenolic compound in control plants, with a concentration of 207.74 ± 1.45 µg/g DW. These results suggest that sublethal doses of heavy metals can act as abiotic elicitors, enhancing the production of phenolic compounds in B. polystachyon. The present work has the potential to open up new commercial opportunities in the pharmaceutical industry.

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“…The separation of individual phenolic acids and total phenolic acids was performed by isometric elution and gradient elution, respectively. The following were the chromatographic conditions: First, 0–10 min, 95–80% A and 5–20% C; second, 10–13 min, 80–65% A and 20–35% C; then 13–33 min, 65–50% A and 35–50% C; and then 33–43 min, 50–5% A and 50–95% C; final, 43–53 min, 5–95% A and 95–5% C [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

Rhizosphere Microbiome and Phenolic Acid Exudation of the Healthy and Diseased American Ginseng Were Modulated by the Cropping History

Zhang,

Wei,

et al. 2023

Plants

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The infection of soil-borne diseases has the potential to modify root exudation and the rhizosphere microbiome. However, the extent to which these modifications occur in various monocropping histories remains inadequately explored. This study sampled healthy and diseased American ginseng (Panax quinquefolius L.) plants under 1–4 years of monocropping and analyzed the phenolic acids composition by HPLC, microbiome structure by high-throughput sequencing technique, and the abundance of pathogens by quantitative PCR. First, the fungal pathogens of Fusarium solani and Ilyonectria destructans in the rhizosphere soil were more abundant in the diseased plants than the healthy plants. The healthy American ginseng plants exudated more phenolic acid, especially p-coumaric acid, compared to the diseased plants after 1–2 years of monocropping, while this difference gradually diminished with the increase in monocropping years. The pathogen abundance was influenced by the exudation of phenolic acids, e.g., total phenolic acids (r = −0.455), p-coumaric acid (r = −0.465), and salicylic acid (r = −0.417), and the further in vitro test confirmed that increased concentration of p-coumaric acid inhibited the mycelial growth of the isolated pathogens for root rot. The healthy plants had a higher diversity of rhizosphere bacterial and fungal microbiome than the diseased plants only after a long period of monocropping. Our study has revealed that the cropping history of American ginseng has altered the effect of pathogens infection on rhizosphere microbiota and root exudation.

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Adventitious rhizogenesis in Basilicum polystachyon (L.) Moench callus and HPLC analysis of phenolic acids

Cited by 5 publications

References 33 publications

Polyphenolic Acid Changes in Stem Cuttings of Rosa Cultivars in Relation to Phenological Stage and Rooting Enhancers

Polyphenolic Acid Changes in Stem Cuttings of Rosa Cultivars in Relation to Phenological Stage and Rooting Enhancers

Unveiling the Dual Nature of Heavy Metals: Stressors and Promoters of Phenolic Compound Biosynthesis in Basilicum polystachyon (L.) Moench In Vitro

Rhizosphere Microbiome and Phenolic Acid Exudation of the Healthy and Diseased American Ginseng Were Modulated by the Cropping History

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