Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways

Tian, Wen; Piao, Xuan‐Mei; Yin, Chengri; Jiang, Xiaolong; Sun, Hao-Ding; An, Xiaolong; Jiang, Jun; Lian, Mei-Lan

doi:10.1007/s11626-019-00396-7

Cited by 9 publications

(2 citation statements)

References 26 publications

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“…These showed the important roles of MAPK and NF-κB in anti-inflammation research. In fact, many studies have reported that the inhibition of MAPK or NF-kB activation suppressed LPS-induced inflammation in macrophages (Dong et al, 2019;Kim et al, 2019;Ma et al, 2019;Shin et al, 2019;Somensi et al, 2019;Tian et al, 2019). Similarly, here, we found that b-AP15 inhibited phosphorylation of ERK1/2 and JNK and suppressed inflammatory reactions in THP-1 and mouse peritoneal macrophages.…”

Section: Discussionsupporting

confidence: 83%

Deubiquitinase Inhibitor b-AP15 Attenuated LPS-Induced Inflammation via Inhibiting ERK1/2, JNK, and NF-Kappa B

Zhang

Chai

et al. 2020

Front. Mol. Biosci.

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b-AP15 is a deubiquitinase (DUB) inhibitor of 19S proteasomes, which in turn targets ubiquitin C-terminal hydrolase 5 (UCHL5) and ubiquitin-specific peptidase 14 (USP14). Nuclear factor kappa B (NF-κB) is closely linked to cellular response in macrophages when the organism is in the state of microbial infection, and it acts as a vital part in the mechanism of inflammatory reaction. However, the molecular mechanism by which DUB inhibitors, especially b-AP15, regulates inflammation remains poorly understood. This study aimed to investigate the relationship between b-AP15 and inflammation. The results showed that b-AP15 treatment significantly reduced the amounts of inflammatory indicators, such as tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in lipopolysaccharide (LPS)-stimulated THP-1 and macrophages. Meanwhile, similar results were obtained from in vivo experiments. In addition, b-AP15 also significantly improved the survival rate of sepsis mouse via high-density LPS mediation. Furthermore, b-AP15 also inhibited the ERK1/2 and JNK phosphorylation, increased IκBα levels, and inhibited NF-κB p65 by removing them from the cytoplasm into the nucleus. All these findings suggested that b-AP15 has anti-inflammatory action and acts as a potential neoteric target drug for treating microbial infection.

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Section: Discussionsupporting

confidence: 83%

Deubiquitinase Inhibitor b-AP15 Attenuated LPS-Induced Inflammation via Inhibiting ERK1/2, JNK, and NF-Kappa B

Zhang

Chai

et al. 2020

Front. Mol. Biosci.

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“…Optimizing the culture conditions for O. elatus ARs in the bioreactor can largely increase its production ( Jiang et al, 2021 ). Extracts from cultured O. elatus Ars have demonstrated excellent anti-inflammatory, antioxidant, and antibacterial activities in our previous study ( Tian et al, 2019 ; Jin et al, 2020 ). However, the hepatoprotective effect of O. elatus ARs has not yet been investigated.…”

Section: Introductionmentioning

confidence: 75%

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

et al. 2022

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O. elatus Nakai is a traditional medicine that has been confirmed to exert effective antioxidant and anti-inflammatory functions, and is used for the treatment of different disorders. However, its potential beneficial effects on drug induced hepatotoxicity and relevant molecular mechanisms remain unclear. This study investigated the protective effect and further elucidated the mechanisms of action of O. elatus on liver protection. O. elatus chlorogenic acids-enriched fraction (OEB), which included chlorogenic acid and isochlorogenic acid A, were identified by HPLC-MS/MS. OEB was administrated orally daily for seven consecutive days, followed by a single intraperitoneal injection of an overdose of APAP after the final OEB administration. The effects of OEB on immune cells in mice liver were analyzed using flow cytometry. APAP metabolite content in serum was detected using HPLC-MS/MS in order to investigate whether OEB affects CYP450 activities. The intestinal content samples were processed for 16 s microbiota sequencing. Results demonstrated that OEB decreased alanine aminotransferase, aspartate aminotransferase contents, affected the metabolism of APAP, and decreased the concentrates of APAP, APAP-CYS and APAP-NAC by inhibiting CYP2E1 and CYP3A11 activity. Furthermore, OEB pretreatment regulated lipid metabolism by affecting the peroxisome proliferator-activated receptors (PPAR) signaling pathway in mice and also increased the abundance of Akkermansia and Parabacteroides. This study indicated that OEB is a potential drug candidate for treating hepatotoxicity because of its ability to affect drug metabolism and regulate lipid metabolism.

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Medicinal potential of adventitious root cultures of Hypericum perforatum: study on antioxidant stress and anti‐melanogenic properties

Guo,

Li,

Zhang

et al. 2024

J Sci Food Agric

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BACKGROUNDAdventitious root (AR) culture is an effective method for the production of Hypericum perforatum raw materials. However, the ARs are seldom utilized in practical applications. Therefore, this study investigated the effects of H. perforatum ARs on antioxidative stress and anti‐melanogenesis, aiming for future applications in the development of related products.RESULTSIn the antioxidative stress experiment, tert‐butyl hydroperoxide‐stimulated HepG2 cells were pre‐treated with 12.5–50 μg mL−1 of AR ethanolic extract (HpE), which significantly increased (P < 0.05) cell viability and reduced reactive oxygen species levels. In alcohol‐stimulated mice, pre‐treatment with HpE (2.5–10 mg kg−1) enhanced the activities of superoxide dismutase, catalase, glutathione, ethanol dehydrogenase, and acetaldehyde dehydrogenase, while reducing the levels of alanine aminotransferase, aspartate aminotransferase, and triglycerides. In the anti‐melanogenesis experiment, α‐melanocyte‐stimulating hormone‐stimulated B16‐F10 mouse melanoma cells were pre‐treated with 25–100 μg mL−1 of HpE, resulting in increased tyrosinase activity and melanin content, along with a decrease in the expression levels of microphthalmia‐associated transcription factor, tyrosinase‐related proteins, and tyrosinase.CONCLUSIONHpE efficiently protects HepG2 cells from oxidative stress and safeguards the liver in mice by mitigating oxidative damage. Additionally, HpE inhibits melanin synthesis in B16‐F10 cells by suppressing tyrosinase activity. These findings suggest that H. perforatum ARs hold potential for use in the development of healthy foods, cosmetics, and pharmaceutical products aimed at antioxidative stress and anti‐melanogenesis. © 2024 Society of Chemical Industry.

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Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways

Cited by 9 publications

References 26 publications

Deubiquitinase Inhibitor b-AP15 Attenuated LPS-Induced Inflammation via Inhibiting ERK1/2, JNK, and NF-Kappa B

Deubiquitinase Inhibitor b-AP15 Attenuated LPS-Induced Inflammation via Inhibiting ERK1/2, JNK, and NF-Kappa B

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

Medicinal potential of adventitious root cultures of Hypericum perforatum: study on antioxidant stress and anti‐melanogenic properties

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