Adventitious Roots and Secondary Metabolism

Murthy, Hosakatte Niranjana; Hahn, Eun-Joo; Paek, Kee-Yoeup

doi:10.1016/s1872-2075(08)60035-7

Cited by 111 publications

(47 citation statements)

References 33 publications

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“…The optimum nutrient concentration is a critical determinant in controlling the growth of the cells/organs and the accumulation of secondary metabolites (Murthy et al, 2008). In contrast to our results, Wu et al, (2006) reported that half-strength MS medium was effective to enhance biomass accumulation and phenolic synthesis in adventitious roots culture of Echinacea angustifolia among varied strength MS (0.25-2.0X) media tested.…”

Section: Discussioncontrasting

confidence: 99%

Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in <em>Bacopa monnieri</em>(L.)

et al. 2017

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Abstract:Bacopa monnieri (L.) Wettst. (Plantaginaceae), commonly known as Brahmi, is used in Ayurveda as a medicinal herb, found throughout the Indian subcontinent in wet, damp and marshy areas. The herb yields pharmaceutically active compounds called bacosides. The current investigation was carried out to assess the potential for increasing biomass and the concentration of bacoside A from the in vitro regenerated shoots in different media (MS, B5, NN and N6), the strength of the MS medium (0.25, 0.50, 0.75, 1.0, 1.50 and 2.0X) and carbon source [sucrose, glucose, fructose, maltose, sucrose + glucose (1:1), sucrose + maltose (1:1), glucose + fructose (1:1), glucose + maltose (1:1), fructose + sucrose (1:1) and fructose + maltose (1:1)]. All the culture media were supplemented with 2 mg/L Kinetin (Kin) at pH 5.8. High performance liquid chromatography (HPLC) was used to determine the bacoside A content from the regenerated shoots. Optimum number of adventitious shoots (70.75 shoots per explant), fresh weight (2.344 g), dry weight (0.166 g) and bacoside A content (13.052 mg/g DW) were obtained from the cultures grown in the MS medium. Bacoside A production was highest in the shoots grown in the glucose + fructose combination (15.588 mg/g DW) among the different carbon sources tested. These findings suggest that B. monnieri culture requires full strength MS medium and a moderate level of sucrose. The present study is useful to obtain optimum production of biomass and bacoside A from the in vitro regenerated shoots of B. monnieri.

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Section: Discussioncontrasting

confidence: 99%

Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in <em>Bacopa monnieri</em>(L.)

et al. 2017

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“…Apiaceae roots have the potential to accumulate secondary metabolites, making them a good source for the production of therapeutic compounds. (Ekiert 2000;Martin et al 2008;Murthy et al 2008). Therefore, it is important that successful techniques for the mass culture of Apiaceae Values not significantly different at P = 0.05 (t-Student's test) Fig.…”

Section: Establishment Of Root Culturesmentioning

confidence: 99%

“…The use of differentiated in vitro organ cultures, especially adventitious root cultures, often results in efficient growth and facilitates the stable production of pharmaceutical compounds. Consequently, this technique has been attempted in many plants that have medicinal value (Murthy et al 2008). In our study, the accumulation of selected phenolic acids (RA, CGA, and CA) in the shoots and roots of in vitro regenerated plantlets, and in liquid root cultures, was confirmed using HPTLC and HPLC.…”

Section: Phenolic Acid Accumulation In Biomass Derived From In Vitro mentioning

confidence: 99%

“…This is thought to be a result of metabolism being controlled in a tissue-specific manner. Cultured undifferentiated cells often partially or totally lose their biosynthetic ability to accumulate secondary products (Murthy et al 2008). Consequently, we focused our efforts on selected phenolic acid accumulation in in vitro plant-derived organ cultures.…”

Section: Phenolic Acid Accumulation In Biomass Derived From In Vitro mentioning

confidence: 99%

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Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Thiem

Kikowska

Krawczyk³

et al. 2013

Plant Cell Tiss Organ Cult

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A protocol for in vitro production of genetically uniform populations of the medicinal plant Eryngium planum, rich in selected phenolic acids, has been established. Shoot-tips were collected from axenic seedlings and grown on a Murashige and Skoog basal medium supplemented with 6-Benzyladenine (BA) and Indole-3-acetic acid (IAA). The highest shoot proliferation efficiency (17 shoots per explant) was obtained when 1.0 mg L -1 BA and 0.1 mg L -1 were added. Proliferating shoots were rooted and transferred to soil (89 % frequency of survival). Flow cytometric analysis of intact (field-grown) and microrpropagated plants revealed that all plants were uniform in genome size and had similar DNA contents. Thin-layer chromatography (TLC) analysis indicated that multiple shoots and roots from in vitro-derived plants produced high amounts of phenolic acids, primarily of rosmarinic acid (RA). Levels of phenolic acids in in vitroderived plants were similar to those of intact plants. Furthermore, high-performance liquid chromatography revealed that root cultures in liquid medium accumulated substantial levels of RA. Thus, rapid establishment of in vitro-grown organ cultures of E. planum can also serve as reliable sources for bioactive compounds.

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“…The latter is also a well-known antimicrobial agent against Gram-positive bacteria, used in cosmetics such as toothpastes, mouthwashes and acne preparations (Varvaresou et al 2009). Given the high essential oil yield of Caryopteris adventitious roots, as well as the suitability of this type of biomass for industrial production of plant secondary metabolites (Murthy et al 2008;Baque et al 2012), the established root cultures can be considered a promising, alternative source of Caryopteris oil. Thus, further scale-up experiments in bioreactors are required to evaluate essential oil productivity of the investigated cultures.…”

Section: Resultsmentioning

confidence: 99%

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

et al. 2015

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The chemical composition of hydrodistilled essential oils obtained from aerial parts and roots of selected Caryopteris ('bluebeard') species (C. incana, C. mongolica, Caryopteris 9 clandonensis), as well as the newly established in vitro shoot and adventitious root cultures of the above plants, was analyzed by gas chromatography-mass spectrometry. Essential oil content and composition differed significantly depending on the type of plant material analyzed. Adventitious roots were characterized by the highest essential oil yield, reaching 1.8 % V/DW in Caryopteris 9 clandonensis. Limonene and cedrol were the main components of the essential oil derived from aerial parts of the intact plants (11.9-16.0 and 10.7-10.9 %, respectively), whereas the volatile fractions of the in vivo roots of all species contained large amounts of 3,5-bis(1,1-dimethyl)-phenol (12.9-26.2 %). 1,8-cineole, absent in the intact plant materials, was the dominating volatile constituent of the essential oils obtained from in vitro shoots (24.8-34.2 %). The volatile oil derived from adventitious root cultures consisted primarily of 1-octen-3-ol (19.7-31.5 %) and medicinally relevant diterpenoids: abietatriene and trans-totarol, which were accumulated in considerable quantities, especially in the adventitious roots of C. clandonensis (21.6 and 29.2 %, respectively).

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Adventitious Roots and Secondary Metabolism

Cited by 111 publications

References 33 publications

Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in <em>Bacopa monnieri</em>(L.)

Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in <em>Bacopa monnieri</em>(L.)

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

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