Adventitious shoot formation from carnation stem segments: a comparison of different culture procedures

Watad, A.A.; Ahroni, A.; Zuker, Amir; Shejtman, Hadas; Nissim, Ada; Vainstein, Alexander

doi:10.1016/0304-4238(96)00874-6

Cited by 23 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the liquid medium, while the percentage of shoot-forming carnation petals met expectations, the number of regenerated shoots from those petals was lower than expected. Although the vessels were shaken, this observation may be due to anoxia, with similar results occurring in carnation stems (Watad et al 1996). On the other hand, differences in the regenerative capacity of White Sim petals between the first (8.5 shoots per petal) and the second experiment (2.8 shoots per petal), both performed in 8 g dm -3 agar, can be attributed to seasonal variations in the organogenic potential of tissues, which is higher in spring than in winter.…”

Section: Discussionsupporting

confidence: 72%

Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots

Casanova¹,

Moysset²,

Trillas³

2008

Biologia plant.

View full text Add to dashboard Cite

Carnation plantlets (Dianthus caryophyllus L.) cultured in vitro often develop morphological and physiological anomalies, a phenomenon called hyperhydricity, which impairs their survival ex vitro. When the agar concentration of the growth medium was increased (from 0 to 12 g dm -3 ), thereby reducing water availability, the hyperhydricity of those adventitious shoots regenerated from carnation petals decreased. This was accompanied by a progressive fall in the water content of shoots (94.9 to 91.4 %), fresh mass (from 57.2 to 1.8 mg), number of leaf parenchyma cell layers (from 9.3 to 7.7), and the size of these cells (from 968 to 254 µm 2 ). However, the number of regenerated shoots also decreased (17.7 in 2 g dm -3 agar to 4.3 in 12 g dm -3 ). Similarly, in ventilated tubes, which exhibit a lower relative humidity than tightly closed tubes, shoot organogenesis diminished up to 28 %, in tandem with shoot water content. Thus, relative humidity and water availability in culture vessels do not only influence shoot hyperhydricity in carnations, but also greatly affect adventitious shoot organogenesis.Additional key words: Dianthus caryophyllus, in vitro culture, leaf parenchyma.

show abstract

Section: Discussionsupporting

confidence: 72%

Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots

Casanova¹,

Moysset²,

Trillas³

2008

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…MV, in which microshoots were permanently submerged in the liquid medium without forced air supply and lack of mechanical stress, was characterized with the lowest growth parameters, probably due to asphyxia which outweighed the benefits of better tissue-medium contact (as compared to agar culture) (Watad et al 1996).…”

Section: Bioreactor Systemsmentioning

confidence: 99%

Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

Jesionek

Kokotkiewicz

Wlodarska

et al. 2017

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

“…To facilitate the continued genetic improvement of Dianthus, a range of in vitro tissue culture techniques have been developed. For example, efficient systems have been established for the regeneration of plants from leaf explants and stem segments (Jethwani and Kothari 1996;Watad et al 1996;Kantia and Kothari 2002) and for the induction of direct somatic embryogenesis from leaf and petal cells (Nakano and Mii 1993;Yantcheva et al 1998;Pareek and Kothari 2003;Karami et al 2006). Although anther culture has been carried out for inducing somatic embryogenesis in various plant species (Achar 2002;Germanà 2003;Kikkert et al 2005;Rimberia et al 2005), there have been a few reports on anther culture of Dianthus species (DolcetSanjuan et al 2001).…”

Section: Introductionmentioning

confidence: 97%

Factors affecting somatic embryogenesis in anther cultures of Chinese pink (Dianthus chinensis L.)

Yang

Bao

2008

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

In this study, we aimed to maximize the rates of somatic embryogenesis achievable in anther cultures of Chinese pink (Dianthus chinensis L.) (2n=2x=30). The genotype of the donor plant was found to be a major factor in determining the success rate. Conditions imposed during anther culture (notably medium composition and light conditions) and pretreatments (namely, cold, heat, and mannitol incubations) were also found to influence somatic embryo induction. For example, the highest levels of embryogenic callus induction were achieved when the donor buds had been cold pretreated and the subsequent anther culture was maintained in darkness. Furthermore, there appeared to be an interaction of genotype with culture conditions. Thus, in cultures of the cultivar (cv.) 'Carpet', the highest rates of embryogenesis were obtained when the anthers had received a 5-d heat-shock, but such a thermal treatment did not generally produce a significant effect. Likewise, a 3-d mannitol pretreatment was optimal only for the cross-hybrid line 'HC'. Assessment of the ploidy of the plants regenerated from the anther cultures revealed both diploid and tetraploid plants. Histological and cytological observations showed that all of these (both from n-pollen and 2n-pollen lines) derived from anther wall cells. Spontaneous chromosome doubling was inferred to have occurred during the embryogenic callus culture period.

show abstract

Adventitious shoot formation from carnation stem segments: a comparison of different culture procedures

Cited by 23 publications

References 22 publications

Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots

Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots

Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

Factors affecting somatic embryogenesis in anther cultures of Chinese pink (Dianthus chinensis L.)

Contact Info

Product

Resources

About