Adventitious Shoot Organogenesis and Plant Regeneration from Internode Explants of Paederia foetida L.:A Valuable Medicinal Plant

Behera, Biswaranjan; Behera, Shashikanta; Jena, Padan Kumar; Barik, Durga Prasad; Naik, Soumendra K.

doi:10.13005/bbra/2523

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…However, in these reports, the number of shoots regenerated is quite low to meet the commercial demand. Besides, plant regeneration through adventitious shoot organogenesis of P. foetida has also been reported by Behera et al [14].…”

Section: Introductionmentioning

confidence: 56%

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

2018

J App Biol Biotech

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An efficient plant regeneration protocol through axillary shoot proliferation of nodal explants of a valuable medicinal plant, Paederia foetida, has been developed. Highest shoot multiplication was found on Murashige and Skoog's (MS) medium supplemented with 3.0 mg/l N 6 -benzylaminopurine. Upscaling of shoots was accomplished from axenic nodal explants derived from primary in vitro regenerated shoots on fresh medium of the same composition. Thus, we could obtain 71-76 shoots, in vitro, starting from a single node within 11-12 weeks. These shoots were successfully rooted on ½ MS medium and acclimatized in soil. The biochemical fidelity was assessed by evaluating the antioxidant activities of leaves of both field grown mother plant and micropropagated plants. The antioxidant activities, as carried out by 2, 2-diphenyl-1-picrylhydrazyl and metal chelating assay, of leaves collected from micropropagated plants were at par with activity of leaves collected from mother plant. Most importantly, both sources have significant antioxidant activities compared to the standard, butylated hydroxytoluene. Further, genetic fidelity of the micropropagated plants with that of the mother plant was assessed by inter simple sequence repeats markers, and their true-to-type nature was confirmed on the basis of monomorphic banding profile. This protocol has potential to create large number of clonal plants with similar antioxidant activities as the natural plants and thus could be useful for drug research and development.

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Section: Introductionmentioning

confidence: 56%

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

2018

J App Biol Biotech

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“…The plant is component of a number of Ayurvedic products available in the Indian market. It is also widely used as a food in different states of India including Odisha, West Bengal and Arunachal Pradesh (Behera et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Regeneration of plants from alginate-encapsulated axenic nodal segments ofPaederia foetidaL. - A medicinally important and vulnerable plant species

Behera

Shasmita

et al. 2021

J Plant Biotechnol

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Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl 2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 °C; thus, they could be useful for transfer and exchange of P. foetida germplasm.

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Harnessing the Potential of Plant Tissue Culture Techniques for the Herbal Industry

Dolker,

Kaur,

Behera

et al. 2024

In Vitro Propagation and Secondary Metabolite Production From Medicinal Plants: Current Trends (Part 1)

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Over the past few years, there has been a tremendous global shift of preference toward herbal medicine because of its affordability, accessibility, efficacy, and lesser side effects. The pharmacological and healing properties of the herbs are due to the presence of a wide array of secondary metabolites. These metabolites are biosynthesized through defined pathways and stored in various parts of the plant, like leaf, root, rhizome, bark, and floral parts. In recent years due to the growing realization of the pharmaceutical properties of medicinal plants, they have been subjected to indiscriminate exploitation. Further, the lack of agrotechnology in many cases and the nonavailability of broad genetic diversity provide impediments to their largescale cultivation and improvement. This situation has created a huge gap between the demand and supply of medicinal plants all over the world. Hence, rapidly propagating high valued medicinal plants through unconventional technologies is warranted and will provide high dividends to farmers and the herbal industry. Further, generating large-scale healthy, genetically uniform plants with defined chemical content will facilitate pre-clinical and translational studies. Therefore, efforts in the development of robust in vitro propagation systems for herbal plants can address the core concern of their conservation and large-scale utilization. Studies on cell suspension, hairy root culture, and genetic transformation have provided the desired impetus in metabolic engineering and enhanced their commercial value. The present article highlights some of these developments and provides a futuristic perspective on the subject.

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Adventitious Shoot Organogenesis and Plant Regeneration from Internode Explants of Paederia foetida L.:A Valuable Medicinal Plant

Cited by 3 publications

References 18 publications

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

Regeneration of plants from alginate-encapsulated axenic nodal segments ofPaederia foetidaL. - A medicinally important and vulnerable plant species

Harnessing the Potential of Plant Tissue Culture Techniques for the Herbal Industry

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