Adventitious shoot regeneration from in vitro leaves of Aronia mitschurinii and cotyledons of closely related Pyrinae taxa

Mahoney, Jonathan D.; Apicella, Peter V.; Brand, Mark H.

doi:10.1016/j.scienta.2018.03.062

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…After 8 weeks of culture, A. melanocarpa (UC007) produced an average of seven shoots per explant (Fig. 2C), which was similar to previous results by Mahoney et al (2018). Cotyledon explants from the A. melanocarpa x P. communis crosses initially formed healthy calli, but at a slightly slower rate than A. melanocarpa (UC007) cotyledon explants.…”

Section: Resultssupporting

confidence: 88%

“…Attempts to regenerate some of the progeny via shoot organogenesis from cotyledon explants followed the protocol developed by Mahoney et al (2018). Briefly, the distal twothirds of the seed were excised and cotyledons were separated and transferred with the abaxial surface in contact with the medium in petri dishes (60 • 16 cm) with 10 mL of the medium.…”

Section: Methodsmentioning

confidence: 99%

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Pre- and Postzygotic Barriers Associated with Intergeneric Hybridization between Aronia melanocarpa (Michx.) Elliott x Pyrus communis L. and ×Sorbaronia dippelii (Zabel) CK Schneid. x Pyrus communis

Mahoney¹,

Brand²

2021

horts

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Intergeneric hybridization between Aronia and Pyrus may provide a pathway for developing novel fruit types with larger, sweeter fruits, while maintaining the high levels of biologically health-promoting compounds present in Aronia fruits. Here we describe a deleterious genetic incompatibility, known as hybrid necrosis or hybrid lethality, that occurs in intergeneric F1 hybrids of Aronia melanocarpa x Pyrus communis and ×Sorbaronia dippelii x Pyrus communis. Pollination experiments revealed that maternal A. melanocarpa and ×S. dippelii pistils are compatible with pollen from P. communis. Controlled pollinations using different mating combinations resulted in varying levels of fruit and seed set. Because every combination produced at least some viable seeds, prezygotic incompatibility does not appear to be present. We attempted to recover putative intergeneric progeny via either in vitro germination or in vitro shoot organogenesis from cotyledons. Progeny of putative hybrids from A. melanocarpa x P. communis only survived for a maximum of 14 days before succumbing to hybrid lethality. Regeneration of ×S. dippelii x P. communis was successful for two seedlings that have been maintained for an extended time in tissue culture. These two seedlings have leaf morphologies intermediate between the two parental genotypes. We also confirmed their hybrid status by using AFLPs and flow cytometry. Putative intergeneric hybrids were grown out ex vitro before showing symptoms of hybrid necrosis and dying after 3 months. Eventually micrografts failed, ultimately showing the same symptoms of hybrid necrosis. These results show that intergeneric hybridization is possible between Aronia and related genera in the Rosaceae, but there are postzygotic barriers to hybridity that can prevent the normal growth and development of the progeny.

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Section: Resultssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Pre- and Postzygotic Barriers Associated with Intergeneric Hybridization between Aronia melanocarpa (Michx.) Elliott x Pyrus communis L. and ×Sorbaronia dippelii (Zabel) CK Schneid. x Pyrus communis

Mahoney¹,

Brand²

2021

horts

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“…As a control group, these explants were also cultured in hormone-free MS nutrient medium. Similarly, the effect of TDZ hormone on in vitro production has been previously reported in plants such as Aronia mitschurinii (Mahoney et al, 2018) and Rauvolfia tetraphylla (L.) (Hussain et al, 2018).…”

Section: Resultssupporting

confidence: 62%

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Doğan

2019

Anatolian Journal of Botany

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The aim of this study is to investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then cultured in Murashige and Skoog (1962) (MS) nutrient media containing thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different combinations. The first shoot formation on the explants was observed on day 15. The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip explants and from 2.72 to 32.24 in the nodal explants. The maximum number of shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ + 0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing 0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to aquarium conditions.

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“…Single-bud microshoots of hawthorn showed mobile dependence on classes and concentrations of phytohormones. The scientists working with the various plants point to the necessity of hormones (Aragão et al, 2017;Mahoney et al, 2018;Stevens and Pijut, 2018). Source: Author Development of buds from microshoots on nutrient medium free of hormones was not practically fixed.…”

Section: Resultsmentioning

confidence: 99%

Microclonal Propagation of Crataegus Monogyna Jacq. In Vitro

Zarnadze

Dolidze

Manjgaladze

et al. 2019

CBUP

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We have researched Crataegus Monogina Jacq. microclonal propagation and in vitro mass regeneration processes. For this experiment we used the asleep buds isolated from maternal plants and in vitro cultivated apical and axillary buds as the effective explants. The high coefficient of micropropagation was provided with Gamborg Medium (B5) nutrient medium added alongside the hormones of cytokinin nature. The best result was achieved in the case of introduction of 2-Isopentenyladenine (2-iP) at concentration of 5-10 mcm and Benzilaminopurine (BAP) at concentration of 10-15 mcm. For stimulation of the mictropropagation process it is reasonable to add a small dose of the auxin hormone 1-Naphtylacetic acid (NAA) together with the cytokinins to the nutritive medium. It has been shown that the growth of micropropagation coefficient in pro rata the kinds and concentration of phytohormones. Regenerated microshoots had a good capacity of rooting. The most effective method for rooting purpose was the introduction of Indole-3 -butyric acid (IBA) at concentration of 5mcm into 1 /2 B5 nutrient medium. The rooted regenerant plants successfully adopted in vivo.

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Adventitious shoot regeneration from in vitro leaves of Aronia mitschurinii and cotyledons of closely related Pyrinae taxa

Cited by 9 publications

References 31 publications

Pre- and Postzygotic Barriers Associated with Intergeneric Hybridization between Aronia melanocarpa (Michx.) Elliott x Pyrus communis L. and ×Sorbaronia dippelii (Zabel) CK Schneid. x Pyrus communis

Pre- and Postzygotic Barriers Associated with Intergeneric Hybridization between Aronia melanocarpa (Michx.) Elliott x Pyrus communis L. and ×Sorbaronia dippelii (Zabel) CK Schneid. x Pyrus communis

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Microclonal Propagation of Crataegus Monogyna Jacq. In Vitro

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