Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L.

Zuker, Amir; Ahroni, A.; Shejtman, Hadas; Vainstein, Alexander

doi:10.1007/s002990050318

Cited by 20 publications

(6 citation statements)

References 8 publications

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“…In this article, we reported a preliminary assessment of genetic diversity among species and hybrids of the genus Gypsophila using molecular markers. The aim of our work was to get further insight into the genetic diversity and relatedness of a number of Gypsophila commercial varieties known to show very little variation (Zuker et al 1997) thus putatively obtaining reliable data on the now unknown origin of most commercial hybrids. The characterization of the genetic structure of Gypsophila may in our opinion be very useful for the construction of breeding strategies yielding the selection and the introduction in the market of new attractive varieties.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the widespread male sterility, new varieties are obtained artificially from wild species through in vitro vegetative propagation and selection of clonal variants. The most common methods used for plant production are micropropagation from shoot-tips explants (Han et al 1991;Lee & Bae 1999;Rady 2006) or regeneration from callus and cell suspension cultures (Salman 2002), leaf explants (Zuker et al 1997), stem segments (Ahroni et al 1997), and more recently, induced mutagenesis with gamma irradiation and collection of lateral buds (Barakat & El-Sammak 2011) and by using bioreactors (Wang et al 2013). Moreover, induction systems for in vitro flower production have been developed (Rady 2006;Kanchanapoom et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Characterization ofGypsophilaspecies and commercial hybrids with nuclear whole-genome and cytoplasmic molecular markers

Calistri

Buiatti

Bogani

2014

Plant Biosystems - An International Journal Dealing with all As

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The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization ofGypsophilaspecies and commercial hybrids with nuclear whole-genome and cytoplasmic molecular markers

Calistri

Buiatti

Bogani

2014

Plant Biosystems - An International Journal Dealing with all As

View full text Add to dashboard Cite

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“…Often mature tissue exhibits a lower shoot regenerative competence. For example, the regeneration competence was much higher in Gypsophila paniculata when younger rather than older leaf explants were used (Zuker et al, 1997). Conversely, cultures initiated on the later date in this study were maintained longer in vitro before the shoot induction study.…”

Section: Resultsmentioning

confidence: 88%

“…Differential response to shoot promoting treatments even between closely related genotypes is a commonly reported phenomenon. For example, signifi cant differences were observed in regeneration response between six cultivars of Gypsophila paniculata L. (Zuker et al, 1997). By the way of comparison, Stimart and Harbage (1989) reported a maximum shoot formation rate in Liatris spicata of 19.8 shoots per explant as induced by 2.7 µM BA.…”

Section: Resultsmentioning

confidence: 99%

Explant Initiation Date and BA Concentration Influence Shoot Proliferation in vitro of Two Liatris Interspecific Hybrids

Ault¹

2004

HortSci

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Shoot proliferation cultures were established in vitro using flower-stem explants from two different interspecific hybrid plants of Liatris. Explants taken on two dates from field-grown plants were successfully established and axillary shoot growth promoted on a medium consisting of Murashige and Skoog basal salts and vitamins with 30 g·L-1 sucrose, 1.0 μm BA, and 7.0 g·L-1 agar, with a medium pH = 5.7. Initial explant contamination rates were significantly higher among explants collected later in the growing season. Addition of BA (1.0, 2.0, 4.0, 8.0, or 16.0 μm) improved shoot formation compared to the control for both plants. Proliferation rates differed between the dates of establishment, the plants, and the BA treatments. Shoots rooted readily in medium without PGRs or with 1.0, 2.0, 4.0, or 8.0 μm K-IBA. Overall rooting was 88%. About 90% of the plants rooted in the presence of 1.0 μm K-IBA were successfully established in the greenhouse. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

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“…The phenomenon that different varieties or genotypes show differences in regeneration efficiencies had been well documented in various species (Caboni et al 1999;Zuker et al 1997). The phenomenon that different varieties or genotypes show differences in regeneration efficiencies had been well documented in various species (Caboni et al 1999;Zuker et al 1997).…”

Section: Shoot Regenerationmentioning

confidence: 99%

Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.)

Liu

Bao

2003

Plant Cell Rep

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Adventitious shoots were successfully regenerated from leaf explants of in vitro cultures of Platanus acerifolia Willd. The leaves of three clones (genotypes), designated as PH1, PH2 and PC, respectively, were wounded by three to four transverse cuts through the midvein and cultured on 26 media based on Murashige and Skoog (MS) basal medium, containing different concentrations of 6-benzylaminopurine (BAP) in combination with different concentrations of indole-3-butyric acid (IBA). The highest regeneration rate (>90%) and the largest number of shoot clumps per regenerating leaf (>4 shoot clumps/explant) were obtained with leaves of genotype PH2 cultured on MS basal medium supplemented with 17.76 mM BAP and 4.92 mM IBA. The other two genotypes, PH1 and PC, showed very low capability of shoot regeneration (<10%) on all the media tested. Shoots on leaf explants originated mainly from callus that developed around the cut end of petioles and along the cuts across the midvein. The regenerated shoots were micropropagated, rooted and transplanted to the soil successfully.

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Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L.

Cited by 20 publications

References 8 publications

Characterization ofGypsophilaspecies and commercial hybrids with nuclear whole-genome and cytoplasmic molecular markers

Characterization ofGypsophilaspecies and commercial hybrids with nuclear whole-genome and cytoplasmic molecular markers

Explant Initiation Date and BA Concentration Influence Shoot Proliferation in vitro of Two Liatris Interspecific Hybrids

Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.)

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