Adventive Embryony in Tissue Cultures of the Wild Carrot, Daucus Carota

Halperin, Walter; Wetherell, D. F.

doi:10.2307/2440296

Cited by 118 publications

(44 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Coconut milk supplementation as an additive in combination with BAP did not support multiplication of shoot either directly or indirectly in O. indicum. This observation supports the past reports [40,41] .…”

Section: Coconut Milksupporting

confidence: 94%

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

Bansal¹,

Gokhale²

2012

Recent Advances in Plant in Vitro Culture

View full text Add to dashboard Cite

Section: Coconut Milksupporting

confidence: 94%

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

Bansal¹,

Gokhale²

2012

Recent Advances in Plant in Vitro Culture

View full text Add to dashboard Cite

“…Since plant growth hormones may also modify membrane permeability (7) and, especially, alter the uptake of other hormones (5), (18); others used kinetin (9). Excised zygotic embryos, especially if immature, profited from either coconut milk (24) or cytokinins (14).…”

Section: Discussionmentioning

confidence: 99%

Hormonal Control of Somatic Embryo Development from Cultured Cells of Caraway

Ammirato¹

1977

Plant Physiol.

137

View full text Add to dashboard Cite

The effects of abscisic acid, zeatin, and gibberellic acid on the development of somatic embryos from cultured cells of caraway (Carum carvi L.) were observed.Somatic embryos complete development on a basal medium without exogenous hormones, but some are subject to developmental abnormalities induding malformed cotyledons and accessory embryos. Both zeatin and gibberellic acid, especialy in combination, stimulate growth and increase the frequency of aberrant forms. Zeatin causes the formation of multiple shoots, leafy and abnormal cotyledons, and in the dark, enlarged hypocotybls; gibberelic acid effects root elongation, polycotyledony, and some caflus formation. In contrast, abscisic acid, at concentrations which do not inhibit embryo maturation, selectively suppresses abnormal proliferations. With abscisic acid, and especially in the dark, a high percentage of embryos complete development with two fleshy cotyledons on unelongated axes free of accessory embryos.In the light, zeatin eliminates abscisic acid inhibition while gibberellic acid only partially counters its effect, promoting elongated radicles and green rather than white cotyledons. In the dark, zeatin in combination with abscisic acid stimulates extensive callusing. Gibberellic acid does not reverse the effects of abscisic acid but rather enhances them and can counter the disruptive effects of zeatin.The results demonstrate that the balance between abscisic acid on the one hand and zeatin and gibberellic acid on the other can effectively control somatic embryo development and either disrupt or ensure normal maturation.Embryos can now be readily grown from cultured cells of certain angiosperms such as caraway (2) or carrot (3) following a simple sequential procedure. These somatic embryos are structurally comparable to zygotic embryos (19): they pass through the same globular, heart-shaped and torpedo stages as do seed embryos and give rise to axes with a root apex at one pole and cotyledons and a shoot apex at the other.In populations of somatic embryos, there is a range of aberrant forms in addition to dicotyledonous embryos: embryos with too few, too many, or malformed cotyledons or callused shoots; those with accessory embryos along the hypocotyl; and multiple embryos with many root-shoot axes arising from one morphogenetic unit. The frequency of occurrence of these unusual forms can vary from experiment to experiment or from culture to culture but is generally high enough to be in contrast to the uniformity seen in zygotic embryos where, with the exception of ISupported by a Barnard College Research Grant. differences in length, the frequency of abnormal forms is rather low. Why does the course of development proceed normally in zygotic and some somatic embryos while in others it is diverted? It has been suggested (3) that these changes may be related to imbalances in the culture medium or environment. If that is the case, modifications in these parameters may serve to reestablish a balance and consequently foster more normal embryogenesi...

show abstract

“…The response was found to be the best with 1.0 mg/l of 2,4-D. The use of the synthetic auxin, 2,4-D for induction of somatic embryos can be traced back as early as 1964 (Halperin and Wetherell 1964). Afterwards, 2,4-D has been used as somatic embryo inducer in a broad range of species (Yu et al 2000, Puchooa 2004, Raghavan 2004.…”

Section: Methodsmentioning

confidence: 99%

High Frequency in vitro Propagation Through Somatic Embryogenesis of Iphigenia indica Kunth et Benth, an Endangered Medicinal and Colchicines Yielding Herb of Commercial Interest

Mukhopadhyay

2008

CYTOLOGIA

View full text Add to dashboard Cite

Summary An efficient and rapid protocol has been reported for the first time for plant regeneration through somatic embryogenesis from corm and young corm buds of Iphigenia indica Kunth et Benth, an important colchicine yielding threatened medicinal plant of Colchicaceae. Soft, friable and well proliferating callus was initiated in Murashige and Skoog's modified basal medium (MS) supplemented with 3% (w/v) sucrose, 0.05 mg/l (w/v) each of nicotinic acid, thiamine-HCl, pyridoxineHCl, ascorbic acid, 0.1 mg/l (w/v) glutamine, 0.25% (w/v) Gelrite ® and a combination of a-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) (2.0/0.5 mg/l). Such callus became nodular and embryonic in nature after transferring to the same MS modified medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) (1.0 mg/l). The globular embryos were observed in this medium after 4 weeks in culture. The transition of globular stage occurred when embryonic callus mass was shifted to half strength of MS medium containing 0.1 mg/l kinetin with production of maximum percentage of embryos showing distinct epicotyl and hypocotyl differentiation (65.4Ϯ0.46). These growing embryos developed into complete plants with well-formed root and shoot systems in the same medium containing 1% (w/v) sucrose. All the regenerates showed stability in chromosome number (2nϭ22) with 87.4% survival rate after 2-3 weeks of hardening. High Pressure Liquid Chromatography (HPLC) analysis revealed higher amount of colchicine in the regenerated corm-like bulbous structures than in vivo corms. However, both regenerated and in vivo shoots contained negligible amount of colchicine.

show abstract

Adventive Embryony in Tissue Cultures of the Wild Carrot, Daucus Carota

Cited by 118 publications

References 0 publications

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

Hormonal Control of Somatic Embryo Development from Cultured Cells of Caraway

High Frequency in vitro Propagation Through Somatic Embryogenesis of Iphigenia indica Kunth et Benth, an Endangered Medicinal and Colchicines Yielding Herb of Commercial Interest

Contact Info

Product

Resources

About