Adverse and beneficial effects in Chinese hamster lung fibroblast cells following radiofrequency exposure

Sannino, Anna; Zeni, Olga; Romeo, Stefania; Massa, Rita; Scarfì, Maria Rosaria

doi:10.1002/bem.22034

Cited by 23 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, Sannino et al ( 42 ) looked at mitomycin C-induced chromosomal fragility in peripheral lymphocytes from 12 MRI workers whose exposure was limited to movement in the SMF. They found a high worker-to-worker variability, but no specific exposure assessment was done with regard to these effects.…”

Section: Discussionmentioning

confidence: 99%

Assessing Exposures to Magnetic Resonance Imaging’s Complex Mixture of Magnetic Fields for In Vivo, In Vitro, and Epidemiologic Studies of Health Effects for Staff and Patients

Frankel

Wilén

Mild

2018

Front. Public Health

View full text Add to dashboard Cite

A complex mixture of electromagnetic fields is used in magnetic resonance imaging (MRI): static, low-frequency, and radio frequency magnetic fields. Commonly, the static magnetic field ranges from one to three Tesla. The low-frequency field can reach several millitesla and with a time derivative of the order of some Tesla per second. The radiofrequency (RF) field has a magnitude in the microtesla range giving rise to specific absorption rate values of a few Watts per kilogram. Very little attention has been paid to the case where there is a combined exposure to several different fields at the same time. Some studies have shown genotoxic effects in cells after exposure to an MRI scan while others have not demonstrated any effects. A typical MRI exam includes muliple imaging sequences of varying length and intensity, to produce different types of images. Each sequence is designed with a particular purpose in mind, so one sequence can, for example, be optimized for clearly showing fat water contrast, while another is optimized for high-resolution detail. It is of the utmost importance that future experimental studies give a thorough description of the exposure they are using, and not just a statement such as “An ordinary MRI sequence was used.” Even if the sequence is specified, it can differ substantially between manufacturers on, e.g., RF pulse height, width, and duty cycle. In the latest SCENIHR opinion, it is stated that there is very little information regarding the health effects of occupational exposure to MRI fields, and long-term prospective or retrospective cohort studies on workers are recommended as a high priority. They also state that MRI is increasingly used in pediatric diagnostic imaging, and a cohort study into the effects of MRI exposure on children is recommended as a high priority. For the exposure assessment in epidemiological studies, there is a clear difference between patients and staff and further work is needed on this. Studies that explore the possible differences between MRI scan sequences and compare them in terms of exposure level are warranted.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessing Exposures to Magnetic Resonance Imaging’s Complex Mixture of Magnetic Fields for In Vivo, In Vitro, and Epidemiologic Studies of Health Effects for Staff and Patients

Frankel

Wilén

Mild

2018

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Most of them refers to RF, but data on extremely low frequencies have also been reported 17–19 . Concerning RF, the phenomenon has been observed both at frequencies and signals in use for telecommunications (900–1950 MHz; continuous wave, GSM, UMTS signals) 1,10,20–23 and at extremely high frequencies (42.2 GHz) 24 . In all cases, pre-exposure significantly reduced the damage induced by a large variety of chemical and physical agents with different action mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…The exposure of SH-SY5Y cell cultures to 1950 MHz, UMTS electromagnetic field was completed by means of a well-established set-up, specifically designed and realized to gain highly controlled electromagnetic and environmental conditions, as widely reported in our previous papers 10,12,39 .…”

Section: Methodsmentioning

confidence: 99%

“…The position of samples inside the waveguides was optimized through numerical dosimetry simulations, to maximize exposure efficiency and electric field uniformity 10,12,13 . In such simulations, the rectangular waveguide (inner transversal dimensions: 109.2 mm × 54.6 mm) was modelled as perfectly conductive with the longitudinal axis and narrow side horizontal, to achieve the condition of an unperturbed (empty waveguide) E vector, parallel to the sample layer.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Protective effect of 1950 MHz electromagnetic field in human neuroblastoma cells challenged with menadione

Falone

Sannino

Romeo

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

This study aims to assess whether a 1950 MHz radiofrequency (RF) electromagnetic field could protect human neuroblastoma SH-SY5Y cells against a subsequent treatment with menadione, a chemical agent inducing DNA damage via reactive oxygen species formation. Cells were pre-exposed for 20 h to specific absorption rate of either 0.3 or 1.25 W/kg, and 3 h after the end of the exposure, they were treated with 10 µM menadione (MD) for 1 h. No differences were observed between sham- and RF-exposed samples. A statistically significant reduction in menadione-induced DNA damage was detected in cells pre-exposed to either 0.3 or 1.25 W/kg (P < 0.05). Moreover, our analyses of gene expression revealed that the pre-exposure to RF almost inhibited the dramatic loss of glutathione peroxidase-based antioxidant scavenging efficiency that was induced by MD, and in parallel strongly enhanced the gene expression of catalase-based antioxidant protection. In addition, RF abolished the MD-dependent down-regulation of oxoguanine DNA glycosylase, which is a critical DNA repairing enzyme. Overall, our findings suggested that RF pre-exposure reduced menadione-dependent DNA oxidative damage, most probably by enhancing antioxidant scavenging efficiency and restoring DNA repair capability. Our results provided some insights into the molecular mechanisms underlying the RF-induced adaptive response in human neuroblastoma cells challenged with menadione.

show abstract

“…As such, people are continuously exposed to such non-ionizing radiation: although it cannot produce charged ions when passing through human body, it can induce charge movements which can affect active transport across cell membranes, cause oxidative stress and interfere with DNA [1][2][3][4][5]. Although the scientific debate concerning adverse health effects of EMFs has been developed for many years, today controversial results still occur [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

DNA-Related Modifications in a Mixture of Human Lympho-Monocyte Exposed to Radiofrequency Fields and Detected by Raman Microspectroscopy Analysis

2019

View full text Add to dashboard Cite

Human exposure to electromagnetic fields (EMFs) has risen considerably during the last decades, because of the industrial and technical development and the consequent increase of artificial EMFs sources. In particular, blood is largely involved in the environmental EMF exposure, because it is located everywhere in the human body. Lympho-monocyte cells are blood components that protect the human organism against infections. In this study, we investigate biochemical changes in lympho-monocyte cells extracted from human peripheral blood after exposure to EMFs at 1.8 GHz frequency and 200 V/m electric field strength for times ranging from 5 to 20 h inside a reverberation chamber. Some mixtures of cells, coming from many human subjects, were exposed and successively investigated by means of Raman micro-spectroscopy technique and principal components analysis. The spectral analysis was able to detect variations of the biochemical composition of the nucleus of exposed cells. Such modifications are mainly detectable as an intensity decrease of some DNA and nucleic acid Raman peaks with respect to the intensity of some protein peaks and they were most evident in the case of 20 h exposed samples. These results were in agreement with the increase of reactive oxygen species (ROS) production, observed in the exposed cells. Overall, the obtained results point out that EMFs exposure may induce modifications of the DNA in some blood cells of long-term exposed people.

show abstract

Adverse and beneficial effects in Chinese hamster lung fibroblast cells following radiofrequency exposure

Cited by 23 publications

References 51 publications

Assessing Exposures to Magnetic Resonance Imaging’s Complex Mixture of Magnetic Fields for In Vivo, In Vitro, and Epidemiologic Studies of Health Effects for Staff and Patients

Assessing Exposures to Magnetic Resonance Imaging’s Complex Mixture of Magnetic Fields for In Vivo, In Vitro, and Epidemiologic Studies of Health Effects for Staff and Patients

Protective effect of 1950 MHz electromagnetic field in human neuroblastoma cells challenged with menadione

DNA-Related Modifications in a Mixture of Human Lympho-Monocyte Exposed to Radiofrequency Fields and Detected by Raman Microspectroscopy Analysis

Contact Info

Product

Resources

About