Adverse effect of blood group ABO mismatching on corneal epithelial cells

Dua, Harminder S.; Chan, Joanna Shi-En; Gomes, José Álvaro Pereira; Azuara‐Blanco, Augusto

doi:10.1016/s0140-6736(05)61448-1

Cited by 6 publications

(6 citation statements)

References 5 publications

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“…In a preliminary study we had demonstrated that cultured corneal epithelial cells readily agglutinated when treated with antibody against their respective blood group 25. In the present study, we observed lysis when corneal epithelial cells of blood group A and B donors were incubated in antiserum against A and B antigen respectively, only when the antiserum was not heat inactivated—that is, contained complement.…”

Section: Discussionsupporting

confidence: 55%

“…Blood group antibodies are mainly of the IgM class, which is generally not detectable in tears and aqueous 3738 Thus, even though epithelial cell agglutination with associated cell death25 and cell lysis as shown in this study can occur by ABO mismatching, it is unlikely that these can occur in vivo. These observations with regard ABO mismatching and corneal epithelium, add another dimension to the “immune privilege” of the cornea.…”

Section: Discussionmentioning

confidence: 57%

“…In 1998 we provided preliminary evidence to demonstrate in vitro agglutination of corneal epithelial cells following ABO mismatching 25. The aim of the present study was to ascertain whether ABO mismatching causes corneal epithelial cell lysis in vitro and to quantify the degree of cell lysis in relation to the concentration of antibody applied.…”

mentioning

confidence: 90%

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Effect of ABO blood group mismatching on corneal epithelial cells: an in vitro study

Hs²,

Powell-Richards³

et al. 2001

British Journal of Ophthalmology

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 57%

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Effect of ABO blood group mismatching on corneal epithelial cells: an in vitro study

Hs²,

Powell-Richards³

et al. 2001

British Journal of Ophthalmology

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“…In addition, the antigen-associated blood type is present in corneal epithelial cells (17, 18). Thus, to reduce immune rejection, the cornea from a living relative that is matched for blood type was used for the donor of epithelium in all cases.…”

Section: Discussionmentioning

confidence: 99%

The Effect of In Vivo Grown Corneal Epithelium Transplantation on Persistent Epithelial Defects with Limbal Stem Cell Deficiency

et al. 2008

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We report our experience with corneal epithelium, grown in vivo, transplantation in three patients with persistent epithelial defect (PED). The three patients had ocular surface disease unresponsive to standard treatments and were therefore chosen for transplantation. They underwent transplantation of epithelial sheets, grown in vivo, to the most affected eye. In vivo cultivation was carried out in the cornea of a living related donor. After epithelialization was completed, the epithelium grown on an amniotic membrane was harvested gently; it was then transplanted into the patient's eye after debridement of fibrovascular tissue. The cultivated epithelium was completely epithelialized by 2 weeks; it was well-differentiated with well-formed hemidesmosome. On immunohistochemical staining, p63, connexin 43, and Integrin β4 were expressed in the cells on the epithelial sheet. The PED was covered completely and maintained for 4 weeks in all cases. However, corneal erosion recurred after 5 weeks in two cases. This novel technique demonstrates the corneal epithelial cells can be expanded in vivo successfully on denuded amniotic membrane of a healthy cornea and harvested safely. A corneal epithelial sheet, grown in vivo, can be transplanted to treat eye with a severe ocular surface disease, such as total limbal deficiency.

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“…If only parents are available, then HLA matching is not necessary, since the recipient will be a haplotype. Since expression of blood-group antigens has been demonstrated on epithelial cells, 25 ABO matching is also advisable when there are several potential donors available.…”

Section: Donor Selectionmentioning

confidence: 99%