Adverse effects of human pulps after direct pulp capping with the different components from a total-etch, three-step adhesive system

Accorinte, Maria de Lourdes Rodrigues; Loguércio, Alessandro Dourado; Reis, Alessandra; Muench, Antônio; Araújo, Vera Cavalcanti de

doi:10.1016/j.dental.2004.08.008

Cited by 68 publications

(50 citation statements)

References 36 publications

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“…In addition, adhesive systems have also been suggested for use as direct pulp capping materials [12]. However, it has been believed that resin systems are inferior to calcium hydroxide-based cements, including MTA [13]. The exception would be a methyl methacrylate-/tributylborane- (MMA/TBB-) based adhesive system, which is known commercially as Super-Bond C&B (SB-C&B) in Japan and C&B Metabond in USA.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts

et al. 2014

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The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and SuperBond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survival was evaluated by MTS assays. In freshly mixed materials, average ± standard deviation % cell viabilities were 40.2 ± 14.0%, 43.7 ± 16.0%, 72.9 ± 12.7%, and 66.0 ± 13.6% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. There was no statistical difference in cell viabilities among material groups, whereas in set materials, the cell viabilities were 48.7 ± 14.8%, 37.2 ± 10.6%, 46.7 ± 15.2%, and 100 ± 21.9% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. Super-Bond C&B showed more cell viabilities than the other three material groups ( < 0.05). The four vital pulp therapy materials had similar cytotoxicity when the materials were fresh. Super-Bond C&B was less cytotoxic than Dycal, Life, and ProRoot MTA after the materials were set, which suggests the use of SB-C&B in future in vivo clinical investigations.

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Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts

et al. 2014

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“…In vitro and in vivo studies have demonstrated that the cytotoxic effects of these systems depend on the amount of unreacted resin monomers, which can be influenced by various factors (3,7), including the degree of conversion, which represents the number of double carbon bonds consumed during the chemical reaction (8,9). Even under sufficient light intensity, an amount of residual monomer can be recorded (10).…”

Section: Introductionmentioning

confidence: 99%

“…In the first instance, they are highly reactive, and then half the unreacted radicals are converted into polymers within 1-2 days. The free monomers that remain after polymerization can be released into the saliva (12) or diffuse through the dentinal tubules towards the pulp (13), and are capable of causing inflammation of variable intensity, or even pulp necrosis (3).…”

Section: Introductionmentioning

confidence: 99%

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

et al. 2009

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The purpose of this study was to evaluate the potential cytotoxicity of Adper Single Bond 2 (SB) simplified etch-and-rinse adhesive system in alveolar macrophage cultures, as a function of the post-polymerization time and duration of immersion in the culture medium for preparation of extracts, by observing the levels of nitric oxide (NO) release and cell survival rate (MTT assay). Wistar rat alveolar macrophages were exposed to 200 µL of extracts obtained from 24-or 72-h immersion of adhesive samples in culture medium (RPMI), immediately or 24 h after polymerization. Fresh RPMI and E. coli lipopolysaccharides were used as negative and positive controls, respectively. The cells were placed in a humidified incubator for 24 h. The results were analyzed by the Student's-t test (α=5%). The amount of NO produced and viable cells were significantly different (p<0.05) between the experimental and the control groups, showing that, irrespective of the post-polymerization time and duration of immersion in the culture medium, the adhesive system caused intense cytotoxicity to the macrophages. The cytotoxic effects were not statistically different (p<0.05) among the experimental groups. In conclusion, chemical components released from SB in aqueous environment were highly toxic to cell culture and thus an inflammatory pulpal response should be considered during the clinical application of dental adhesives.

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“…It appears that material choices, such as zinc phosphate cement, 5 amalgam, 5 zinc oxide eugenol cements, 6 polycarbonate cements, 7 glass ionomer cements, [8][9][10][11] resin adhesives [12][13][14] and cyanoacrylates 15 do not lead to predictable success for the treatment of exposed pulps. Outcomes with calcium hydroxide were superior but unpredictable for carious pulp exposures.…”

Section: Introductionmentioning

confidence: 99%

Pulp Capping with Mineral Trioxide Aggregate (MTA): A Retrospective Analysis of Carious Pulp Exposures Treated by Undergraduate Dental Students

Miles¹,

Gluskin²,

Chambers³

et al. 2010

Operative Dentistry

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©Operative Dentistry, 2010, 35-1, 20-28 JP Miles • AH Gluskin D Chambers • OA Peters Clinical RelevanceCarious pulp exposures present a therapeutic challenge for clinicians. Mineral trioxide aggregate (MTA) is a treatment option that may provide successful outcomes for the capping of carious pulp exposures in adult patients. However, the success measured as pulp survival over a period of one and two years of pulp caps performed by undergraduate dental students may be low. This study provides data regarding the impact of exposure sizes and other pre-operative variables on outcomes of MTA pulp caps in adults. SUMMARYThe current study estimated pulpal vitality after MTA pulp caps were performed by undergraduate student clinicians. At recall after 12 to 27 months, 51 pulp caps were clinically and radiographically assessed. Kaplan-Meier analyses were used to estimate overall success at 12 and 24 months, determined as the presence of a vital pulp, as well as impact of preoperative variables on pulp vitality at recall. Overall, one-year pulp survival was 67.7%, while the two-year survival rate was 56.2%. Tarone-Ware statistics indicated that neither age of the patient nor size of the exposure ("minimal" or "moderate") and the amount of bleeding ("none," "minimal" or "moderate") had a significant effect on survival rates.Within the limitations of the current study on procedures performed by student clinicians, it may be concluded that, for MTA pulp caps applied to carious exposures in adults, certain preoperative conditions-patient age, exposure size and amount of bleeding-are not predictive of clinical outcome. Considering the comparatively low success rate for the current cohort, more research is needed to define predictive criteria for successful pulp capping with MTA.

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Adverse effects of human pulps after direct pulp capping with the different components from a total-etch, three-step adhesive system

Cited by 68 publications

References 36 publications

In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts

In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

Pulp Capping with Mineral Trioxide Aggregate (MTA): A Retrospective Analysis of Carious Pulp Exposures Treated by Undergraduate Dental Students

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