Adverse Effects of Subchronic Dose of Aspirin on Reproductive Profile of Male Rats

Abstract

Aspirin (acetylsalicylic acid) is widely used for cardiovascular prophylaxis and as anti-inflammatory pharmaceutical. An investigation was carried out to evaluate the influence of subchronic dose of aspirin on reproductive profile of male rats, if any. Experimental animals were divided into three groups: control and aspirin subchronic dose of 12.5 mg/kg for 30 days and 60 days, respectively, while alterations in sperm dynamics, testicular histopathological and planimetric investigations, body and organs weights, lipid profiles, and hematology were performed as per aimed objectives. Subchronic dose of aspirin reduced sperm density, count, and mobility in cauda epididymis and testis; histopathology and developing primary spermatogonial cells (primary spermatogonia, secondary spermatogonia, and mature spermatocyte) count were also significantly decreased in rats. Hematological investigations revealed hemopoietic abnormalities in 60-day-treated animals along with dysfunctions in hepatic and renal functions. The findings of the present study revealed that administration with subchronic dose of aspirin to male rats resulted in altered reproductive profiles and serum biochemistry.

Aspirin affect on Reproduction of Male Rat an-Overview

“…Aspirin decrease the testicular weight [13], decrease in the weight and nuclear volume of the Leydig cells indicates that Aspirin alters the steroidogenic function of the Leydig cells.…”

“…According to developmental progression at the base of the Seminiferous tubules have Spermatogonia, spermatocytes in the middle, and spermatids near the apex of the Seminiferous epithelium [23]. The aspirin treated rats showed cytological and nuclear degenerative changes in Seminiferous leading to shrinkage to Seminiferous tubules [13].…”

“…Interestingly, subchronic aspirin administration influenced androgen dependent parameters including that of reduced sperm count, motility, and density. The observed reduced sperm activity profile might be an outcome of androgen depletion at target level, particularly in the cauda epididymis, thereby affecting physiological maturation of the sperm [13]. Decrease in activity of hyaluronidase, decrease in number of spermatid and increase in size of spermatocytes nuclei is the sign that aspirin cause impairment on later stage of spermatogenesis.…”

“…One of very worse effect of aspirin is on reproduction of male. It was reported that aspirin caused a significant decrease in the weight of testis of immature rats [13]. Decrease in the activities of sorbitol dehydrogenase and hyaluronidase [14].…”

“…The process of spermatogenesis and the accessory reproductive organs function are dependent on androgen activity. Aspirin administration to normal rats resulted in hypercholesterolemia that's might be due to decreased androgen production, which resulted in accumulation of cholesterol in testes [13]. The impaired sperm dynamics, including spermatogenesis, could be an outcome of aspirin-induced alteration in cholesterol metabolism in testis [16].…”