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Brenner, Gabrielle A.; Brenner, Reuven

doi:10.1017/cbo9780511559426.006

Cited by 35 publications

(41 citation statements)

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“…28 The Petri dish housing the open lPADs (paper-based or plastic-based) is placed on the stage of a Leica MZ16 stereozoom microscope (7.1Â to 230Â magnification range) at an ambient temperature of 22 C. Two methods are used to put worms on the membrane devices: pipetting worm droplets and picking/ insertion of worms. In the pipetting approach, individual L4-stage worms are collected in a droplet of M9 buffer and pipetted directly onto the agar membrane [ Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Caenorhabditis elegans is an important, small model nematode pivotal to experiments on gene regulation, metabolism, ageing, cell signaling, chemical screening, drug discovery, and space flight. [28][29][30][31][32] Compared to its mammalian counterparts, C. elegans is well-suited for high-throughput, large scale biological experiments because of the ease to culture on a diet of Escherichia coli, ability to grow from an egg to an adult within three days, and capacity to produce over 300 progeny. 28,29 In this context, polymer-based microfluidics has emerged as an enabling technology for C. elegans research where on-chip automation has streamlined the steps of worm capture, immobilization, transport, screening, sorting, and/or tracking.…”

Section: Introductionmentioning

confidence: 99%

“…[28][29][30][31][32] Compared to its mammalian counterparts, C. elegans is well-suited for high-throughput, large scale biological experiments because of the ease to culture on a diet of Escherichia coli, ability to grow from an egg to an adult within three days, and capacity to produce over 300 progeny. 28,29 In this context, polymer-based microfluidics has emerged as an enabling technology for C. elegans research where on-chip automation has streamlined the steps of worm capture, immobilization, transport, screening, sorting, and/or tracking. [33][34][35][36] For instance, in the fields of pharmacology and drug discovery, it is possible to trap individual worms within discrete liquid droplets in a microfluidic chip and simultaneously screen them for a range of drug concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing

et al. 2017

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Today, the area of point-of-care diagnostics is synonymous with paper microfluidics where cheap, disposable, and on-the-spot detection toolkits are being developed for a variety of chemical tests. In this work, we present a novel application of microfluidic paper-based analytical devices (μPADs) to study the behavior of a small model nematode, Caenorhabditis elegans. We describe schemes of μPAD fabrication on paper and plastic substrates where membranes are created in agarose and Pluronic gel. Methods are demonstrated for loading, visualizing, and transferring single and multiple nematodes. Using an anthelmintic drug, levamisole, we show that chemical testing on C. elegans is easily performed because of the open device structure. A custom program is written to automatically recognize individual worms on the μPADs and extract locomotion parameters in real-time. The combination of μPADs and the nematode tracking program provides a relatively low-cost, simple-to-fabricate imaging and screening assay (compared to standard agarose plates or polymeric microfluidic devices) for non-microfluidic, nematode laboratories.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing

et al. 2017

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“…Paul͒ and was cultivated as described by Brenner. 20 Briefly, worms were cultivated at 20°C on nematode growth medium ͑NGM͒ agar seeded with Escherichia coli OP50 ͑food͒. Prior to seeding, bacteria were incubated overnight at 37°C.…”

Section: B Worm Culture and Treatmentmentioning

confidence: 99%

A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans

Jiang

Shi

et al. 2009

Biomicrofluidics

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The soil dwelling nematode Caenorhabditis elegans ͑C. elegans͒ is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.

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“…Caenorhabditis elegans has become a well-established model organism since the pioneer work introduced by Brenner in 1963 [1]. Since its establishment as a model organism, C. elegans has been an invaluable tool for biological research.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Platform for the Study of Caenorhabditis elegans

Shi

Wen

Lin

et al. 2011

Topics in Current Chemistry

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Caenorhabditis elegans is a well-established model organism for the study of various biological areas. However, the procedures generally used for this worm are limited by the low throughput, low automation, and imprecise delivery of external stimuli. Microfluidics technology is emerging as an attractive and enabling platform to overcome these problems. In this review, we summarize the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans, and discuss the trends of future development.

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Advertising, memory, and custom

Cited by 35 publications

References 0 publications

Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing

Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing

A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans

Microfluidic Platform for the Study of Caenorhabditis elegans

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